Wavelength-Programmed Solute Release from Photosensitive Liposomes

Bisby, Roger H.; Mead, Carole; Morgan, Christopher G.

doi:10.1006/bbrc.2000.3456

Cited by 98 publications

(71 citation statements)

References 12 publications

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“…Additionally, the shell should keep capsules intact without their destruction during "smart" activities such as drug release. The transport across the shell by using light to modulate the channel's activity is possibly due to the application of photoswitchable copolymers [184][185][186][187] incorporated into lipids bilayers [187] or the doping them with photosensitive amphiphiles [188][189][190][191][192][193][194][195] and using photoswichable lipids (e.g., containing malachite green) [196] or cerasomes with a porous silicate framework containing azobenzene moieties [197]. Light is a useful trigger to manipulate ion channels externally, because does not change the chemical environment of the studied system.…”

Section: Non-fluorescent Prodrugs/converted Into Fluorescent Active Dmentioning

confidence: 99%

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Miksa¹

2016

Med chem (Los Angeles)

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This review highlights the role of fluorescent dyes as active "molecular photoswiches" focusing on the application in bioimaging and anticancer therapies in the field of therapeutic delivery. The author describes the development of prodrugs targeted to specific cell types and polymeric nanocarriers (capsules, micelles and silica nanoparticles) used as fluorescent probes which offer advantages in the integration of "smart" features of fluorescent dyes into synthetic materials. The incorporation of biologically responsive components that cleave upon changes in pH or light irradiation is fundamental to the successful design of such carriers with capabilities to load and release therapeutics specifically at a target site.

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Section: Non-fluorescent Prodrugs/converted Into Fluorescent Active Dmentioning

confidence: 99%

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Miksa¹

2016

Med chem (Los Angeles)

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“…11,12 The principles of phototriggering include photopolymerization of lipids, 13 photosensitization by membrane-anchored hydrophobic probes, [14][15][16] or photoisomerization of photoreactive lipids. 17 To date, none of the lipid formulations developed have been successfully demonstrated for in vivo phototriggering applications.…”

Section: 10mentioning

confidence: 99%

Photo activation of HPPH encapsulated in “Pocket” liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts

Sine¹,

Urban²,

Thayer³

et al. 2014

IJN

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We recently reported laser-triggered release of photosensitive compounds from liposomes containing dipalmitoylphosphatidylcholine (DPPC) and 1,2 bis(tricosa-10,12-diynoyl)- sn -glycero-3-phosphocholine (DC 8,9 PC). We hypothesized that the permeation of photoactivated compounds occurs through domains of enhanced fluidity in the liposome membrane and have thus called them “Pocket” liposomes. In this study we have encapsulated the red light activatable anticancer photodynamic therapy drug 2-(1-Hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) (Ex/Em410/670 nm) together with calcein (Ex/Em490/517 nm) as a marker for drug release in Pocket liposomes. A mole ratio of 7.6:1 lipid:HPPH was found to be optimal, with >80% of HPPH being included in the liposomes. Exposure of liposomes with a cw-diode 660 nm laser (90 mW, 0–5 minutes) resulted in calcein release only when HPPH was included in the liposomes. Further analysis of the quenching ratios of liposome-entrapped calcein in the laser treated samples indicated that the laser-triggered release occurred via the graded mechanism. In vitro studies with MDA-MB-231-LM2 breast cancer cell line showed significant cell killing upon treatment of cell-liposome suspensions with the laser. To assess in vivo efficacy, we implanted MDA-MB-231-LM2 cells containing the luciferase gene along the mammary fat pads on the ribcage of mice. For biodistribution experiments, trace amounts of a near infrared lipid probe DiR (Ex/Em745/840 nm) were included in the liposomes. Liposomes were injected intravenously and laser treatments (90 mW, 0.9 cm diameter, for an exposure duration ranging from 5–8 minutes) were done 4 hours postinjection (only one tumor per mouse was treated, keeping the second flank tumor as control). Calcein release occurred as indicated by an increase in calcein fluorescence from laser treated tumors only. The animals were observed for up to 15 days postinjection and tumor volume and luciferase expression was measured. A significant decrease in luciferase expression and reduction in tumor volume was observed only in laser treated animal groups injected with liposomes containing HPPH. Histopathological examination of tumor tissues indicated tumor necrosis resulting from laser treatment of the HPPH-encapsulated liposomes that were taken up into the tumor area.

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“…[1][2][3][4] Several studies have described the synthesis and properties of photoreactive molecules (primarily lipids) designed to enhance localized drug delivery (triggered release) upon activation by a suitable light source (photo-triggering). [5][6][7][8][9][10][11][12] Photo-triggering typically involves activation of the photosensitive molecules, causing a perturbation (destabilization) of the liposome membrane. 7,13,14 Release mechanisms of liposome-entrapped drugs and pharmaceuticals are generally based on localized membrane destabilization due to structural changes in fatty acid chains 5,7,11,15 (for further information on the mechanism[s] of photo-triggering of the lipid bilayers for enhanced drug delivery, readers are referred to comprehensive reviews).…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7][8][9][10][11][12] Photo-triggering typically involves activation of the photosensitive molecules, causing a perturbation (destabilization) of the liposome membrane. 7,13,14 Release mechanisms of liposome-entrapped drugs and pharmaceuticals are generally based on localized membrane destabilization due to structural changes in fatty acid chains 5,7,11,15 (for further information on the mechanism[s] of photo-triggering of the lipid bilayers for enhanced drug delivery, readers are referred to comprehensive reviews). 9,11,[16][17][18] However, the mechanistic role of photo-activated liposomeencapsulated molecules (drugs or fluorescent solutes) in the release has not been addressed.…”

Section: Introductionmentioning

confidence: 99%

Low-visibility light-intensity laser-triggered release of entrapped calcein from 1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine liposomes is mediated through a type I photoactivation pathway

Puri¹,

Viard²,

Viard³

et al. 2013

IJN

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Wavelength-Programmed Solute Release from Photosensitive Liposomes

Cited by 98 publications

References 12 publications

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Photo activation of HPPH encapsulated in “Pocket” liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts

Low-visibility light-intensity laser-triggered release of entrapped calcein from 1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine liposomes is mediated through a type I photoactivation pathway

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Wavelength-Programmed Solute Release from Photosensitive Liposomes

Cited by 98 publications

References 12 publications

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Photo activation of HPPH encapsulated in&nbsp;&ldquo;Pocket&rdquo; liposomes triggers multiple drug release and tumor cell killing in mouse breast&nbsp;cancer xenografts

Low-visibility light-intensity laser-triggered release of entrapped calcein from 1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine liposomes is mediated through a type I photoactivation pathway

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Photo activation of HPPH encapsulated in “Pocket” liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts