Wax and cutin mutants of Arabidopsis: Quantitative characterization of the cuticular transport barrier in relation to chemical composition

Sadler, Christina; Schroll, Bettina; Zeisler, Viktoria; Waßmann, Friedrich; Franke, Rochus; Schreiber, Lukas

doi:10.1016/j.bbalip.2016.03.002

Cited by 24 publications

(24 citation statements)

References 28 publications

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“…5D). The eca2 mutant has, thus, a reduction in the amounts of both wax and cutin being the likely cause of the increased permeability of the cuticle of eca2 plants (Sadler et al 2016). …”

Section: Resultsmentioning

confidence: 99%

“…eca2 has a strong reduction in both wax and cutin but did not present transcriptional compensatory mechanisms related to this phenotype, which have not been described before (Nawrath et al 2013;Sadler et al 2016). A previous transcriptional analysis of the cuticle mutants lcr, fdh, and bdg revealed a set of DEGs related to the synthesis of wax, cutin, cell wall, and defense response (Voisin et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Total cuticular wax extracts were prepared as described previously (Greer et al 2007). The quantification of wax components was performed as previously described (Sadler et al 2016;Voisin et al 2009). …”

Section: Cuticle Permeability Analysismentioning

confidence: 99%

See 2 more Smart Citations

The Cuticle Mutanteca2Modifies Plant Defense Responses to Biotrophic and Necrotrophic Pathogens and Herbivory Insects

Blanc

Coluccia

L’Haridon

et al. 2018

MPMI

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We isolated previously several Arabidopsis thaliana mutants with constitutive expression of the early microbe-associated molecular pattern–induced gene ATL2, named eca (expresión constitutiva de ATL2). Here, we further explored the interaction of eca mutants with pest and pathogens. Of all eca mutants, eca2 was more resistant to a fungal pathogen (Botrytis cinerea) and a bacterial pathogen (Pseudomonas syringae) as well as to a generalist herbivorous insect (Spodoptera littoralis). Permeability of the cuticle is increased in eca2; chemical characterization shows that eca2 has a significant reduction of both cuticular wax and cutin. Additionally, we determined that eca2 did not display a similar compensatory transcriptional response, compared with a previously characterized cuticular mutant, and that resistance to B. cinerea is mediated by the priming of the early and late induced defense responses, including salicylic acid– and jasmonic acid–induced genes. These results suggest that ECA2-dependent responses are involved in the nonhost defense mechanism against biotrophic and necrotrophic pathogens and against a generalist insect by modulation and priming of innate immunity and late defense responses. Making eca2 an interesting model to characterize the molecular basis for plant defenses against different biotic interactions and to study the initial events that take place in the cuticle surface of the aerial organs.

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“…5D). The eca2 mutant has, thus, a reduction in the amounts of both wax and cutin being the likely cause of the increased permeability of the cuticle of eca2 plants (Sadler et al 2016). …”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Cuticle Mutanteca2Modifies Plant Defense Responses to Biotrophic and Necrotrophic Pathogens and Herbivory Insects

Blanc

Coluccia

L’Haridon

et al. 2018

MPMI

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“…The presence of outer cuticle and its physiology help the microbes to colonize this site. Presence of aliphatic compounds in the cuticle layer determines the physicochemical properties of the leaf surface and renders the permeability and wettability, which facilitate the adherence of microorganisms (Sadler et al 2016). Water permeability of this site may play a vital role in the survival and growth of the epiphytes.…”

Section: Microbial Assembly On Leafmentioning

confidence: 99%

Phyllospheric Microbiomes: Diversity, Ecological Significance, and Biotechnological Applications

Sivakumar

Sathishkumar

Selvakumar

et al. 2020

Sustainable Development and Biodiversity

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The phyllosphere referred to the total aerial plant surfaces (above-ground portions), as habitat for microorganisms. Microorganisms establish compositionally complex communities on the leaf surface. The microbiome of phyllosphere is rich in diversity of bacteria, fungi, actinomycetes, cyanobacteria, and viruses. The diversity, dispersal, and community development on the leaf surface are based on the physiochemistry, environment, and also the immunity of the host plant. A colonization process is an important event where both the microbe and the host plant have been benefited. Microbes commonly established either epiphytic or endophytic mode of life cycle on phyllosphere environment, which helps the host plant and functional communication with the surrounding environment. To the scientific advancement, several molecular techniques like metagenomics and metaproteomics have been used to study and understand the physiology and functional relationship of microbes to the host and its environment. Based on the available information, this chapter describes the basic understanding of microbiome in leaf structure and physiology, microbial interactions, especially bacteria, fungi, and actinomycetes, and their adaptation in the phyllosphere environment. Further, the detailed information related to the importance of the microbiome in phyllosphere to the host plant and their environment has been analyzed. Besides, biopotentials of the phyllosphere microbiome have been reviewed.

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“…Using synthetic microRNAs, Shi et al (2011) simultaneously silenced three Arabidopsis SHN clade members, suggesting the redundant functions of SHN TFs in cutin biosynthesis; the reduced activity of SHN TFs led to a decrease in the cutin load and fusion of floral organs. Further studies demonstrated that the overexpression of SHN TFs often leads to an enhanced tolerance of transgenic plants to abiotic and biotic stresses (Al-Abdallat, Al-Debei, Ayad, & Hasan, 2014;Bao et al, 2017;Kumar et al, 2016;Sadler et al, 2016;Sela et al, 2013;Wang et al, 2012;Yang, Isabel Ordiz, Jaworski, & Beachy, 2011).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions

Shi

Kovalchuk

et al. 2018

Plant Cell & Environment

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Transcription factors regulate multiple networks, mediating the responses of organisms to stresses, including drought. Here, we investigated the role of the wheat transcription factor TaSHN1 in crop growth and drought tolerance. TaSHN1, isolated from bread wheat, was characterized for molecular interactions and functionality. The overexpression of TaSHN1 in wheat was followed by the evaluation of T and T transgenic lines for drought tolerance, growth, and yield components. Leaf surface changes were analysed by light microscopy, SEM, TEM, and GC-MS/GC-FID. TaSHN1 behaves as a transcriptional activator in a yeast transactivation assay and binds stress-related DNA cis-elements, determinants of which were revealed using 3D molecular modelling. The overexpression of TaSHN1 in transgenic wheat did not result in a yield penalty under the controlled plant growth conditions of a glasshouse. Transgenic lines had significantly lower stomatal density and leaf water loss and exhibited improved recovery after severe drought, compared with control plants. The comparative analysis of cuticular waxes revealed an increased accumulation of alkanes in leaves of transgenic lines. Our data demonstrate that TaSHN1 may operate as a positive modulator of drought stress tolerance. Positive attributes could be mediated through an enhanced accumulation of alkanes and reduced stomatal density.

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Wax and cutin mutants of Arabidopsis: Quantitative characterization of the cuticular transport barrier in relation to chemical composition

Cited by 24 publications

References 28 publications

The Cuticle Mutanteca2Modifies Plant Defense Responses to Biotrophic and Necrotrophic Pathogens and Herbivory Insects

The Cuticle Mutanteca2Modifies Plant Defense Responses to Biotrophic and Necrotrophic Pathogens and Herbivory Insects

Phyllospheric Microbiomes: Diversity, Ecological Significance, and Biotechnological Applications

Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions

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