The electrocatalytic oxidation of L-tyrosine (Tyr) was investigated on a carboxylic acid functionalised multiwalled carbon nanotubes modified carbon paste electrode using cyclic voltammetry and amperometry. The surface morphology of the electrodes was studied using field emission (FE)-SEM images, and the interface properties of bare and modified electrodes were investigated by electrochemical impedance spectroscopy (EIS). The influence of the amount of modifier loading and the variation of the pH of the solution on the electrochemical parameters have been investigated. Cyclic voltammetry was carried out to study the electrochemical oxidation mechanism of Tyr, which showed an irreversible oxidation process at a potential of 637.0 mV at modified electrode. The anodic peak current linearly increased with the scan rate, suggesting that the oxidation of Tyr at modified electrode is an adsorption-controlled process. A good linear relationship between the oxidation peak current and the Tyr concentration in the range of 0.8-100.0 μM was obtained in a phosphate buffer solution at pH 7.0 with a detection limit of 14.0± 1.36 nM (S/N=3). The practical utility of the sensor was demonstrated by determining Tyr in spiked cow's milk and human blood serum. The modified electrode showed excellent reproducibility, long-term stability and antifouling effects.