We know you are in there: Conversing with the indigenous gut microbiota

Cheesman, Sarah E.; Guillemin, Karen

doi:10.1016/j.resmic.2006.10.005

Cited by 77 publications

(62 citation statements)

References 44 publications

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“…Recently, molecular crosstalk between gut and the inhabited microbes has become a hot topic in vertebrates. In the absence of microbes, differentiation of gut epithelium is arrested in zebrafish (Bates et al, 2006;Cheesman and Guillemin, 2007). On the other hand, previous reports have confirmed that apolipoproteins have antibacterial activities in mammalian and fish (Srinivas et al, 1990;Singh et al, 1999), and are potentially important effectors of innate immunity in teleost fish (Concha et al, 2004).…”

Section: Discussionmentioning

confidence: 89%

Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development

Xia¹,

Liu²,

Zhou³

et al. 2008

Int. J. Dev. Biol.

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Apo-14 is a fish-specific apolipoprotein and its biological function remains unknown. In this study, CagApo-14 was cloned from gibel carp (Carassius auratus gibelio) and its expression pattern was investigated during embryogenesis and early larval development. The CagApo-14 transcript and its protein product were firstly localized in the yolk syncytial layer at a high level during embryogenesis, and then found to be restricted to the digestive system including liver and intestine in later embryos and early larvae. Immunofluorescence staining in larvae and adults indicated that CagApo-14 protein was predominantly synthesized in and excreted from sinusoidal endothelial cells of liver tissue. Morpholino knockdown of CagApo-14 resulted in severe disruption of digestive organs including liver, intestine, pancreas and swim bladder. Moreover, yolk lipid transportation and utilization were severely affected in the CagApo-14 morphants. Overall, this data indicates that CagApo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.

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Section: Discussionmentioning

confidence: 89%

Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development

Xia¹,

Liu²,

Zhou³

et al. 2008

Int. J. Dev. Biol.

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“…Understanding how host-associated microbiotas assemble requires the use of model systems that reflect natural host community establishment and that allow for the rigorous experimental analysis of the microbiota. Our knowledge of how gut microbial communities assemble and interact with vertebrate hosts is largely derived from a few laboratory model species including mice, rats, and zebrafish (Bäckhed et al, 2005;Cheesman and Guillemin, 2007;Camp et al, 2009). However, the use of lab-reared animals to study these complex and subtle host-microbiota interactions is appropriate only if those interactions in the lab are representative of the interactions that occur in nature.…”

Section: Introductionmentioning

confidence: 99%

“…We have developed gnotobiotic husbandry methods for the zebrafish, and used them to reveal host responses to the gut microbiota including effects on innate immunity, nutrient metabolism, and intestinal epithelial differentiation and renewal (Rawls et al, 2004(Rawls et al, , 2006Bates et al, 2006Bates et al, , 2007Cheesman and Guillemin, 2007;Cheesman et al, 2011;Kanther and Rawls, 2010). Preliminary insights into the membership of the zebrafish gut microbiota have been provided by sequencing libraries of bacterial 16S rRNA genes amplified from pooled intestinal samples from zebrafish reared in laboratory aquaculture facilities (Rawls et al, 2004(Rawls et al, , 2006Bates et al, 2006;Brugman et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Evidence for a core gut microbiota in the zebrafish

et al. 2011

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Experimental analysis of gut microbial communities and their interactions with vertebrate hosts is conducted predominantly in domesticated animals that have been maintained in laboratory facilities for many generations. These animal models are useful for studying coevolved relationships between host and microbiota only if the microbial communities that occur in animals in lab facilities are representative of those that occur in nature. We performed 16S rRNA gene sequence-based comparisons of gut bacterial communities in zebrafish collected recently from their natural habitat and those reared for generations in lab facilities in different geographic locations. Patterns of gut microbiota structure in domesticated zebrafish varied across different lab facilities in correlation with historical connections between those facilities. However, gut microbiota membership in domesticated and recently caught zebrafish was strikingly similar, with a shared core gut microbiota. The zebrafish intestinal habitat therefore selects for specific bacterial taxa despite radical differences in host provenance and domestication status.

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“…Among the most prominent innate immune factors are TLRs, the TLR/IL-1R adaptor protein MyD88 and Nod-like receptors (NLR). 10,11 Adult mice lacking these proteins harbor altered microbiota and are very susceptible to intestinal injury caused by chemicals or intestinal pathogens. 5,[12][13][14] In addition, both B and T cells are indispensable for the regulation of bacterial communities under steady-state and inflammatory conditions.…”

Section: Introductionmentioning

confidence: 99%