Weak acids induce PGE2 production in human oesophageal cells: novel mechanisms underlying GERD symptoms

Sadatomi, Daichi; Kono, Toru; Mogami, Sachiko; Fujitsuka, Naoki

doi:10.1038/s41598-020-77495-z

Cited by 5 publications

(3 citation statements)

References 47 publications

(57 reference statements)

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“…Particularly relevant are the little-studied issues of the influence of humoral factors and their dynamics on the development of GERD during treatment [1,5,8,10,12]. One of the biologically active substances of interest is prostaglandin E 2 , the possible involvement of which in the mechanisms of GERD development is reported in separate works [4,[6][7][8]11]. However, the available data are based on an insufficient number of studies and require further study of the pathogenetic role of prostaglandin E 2 in patients with GERD.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of prostaglandin E2 in the surgical treatment of gastroesophageal reflux disease

Haidarzhi¹

2023

Patologìâ

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Gastroesophageal reflux disease (GERD) is one of the most common gastroenterological diseases. Therefore, the issues of diagnosis and the most effective treatment of GERD are extremely relevant. Achieving a stable positive result of treatment is impossible without taking into account the pathogenetic mechanisms of the development of GERD. Particularly relevant are the little-studied issues of the influence of humoral factors on the development of GERD in the course of treatment. One of the interesting biologically active substances is prostaglandin E2, the possible involvement of which in the mechanisms of the development of GERD is insufficiently reported. The aim of the study is to evaluate the effect of antireflux surgery on the level of prostaglandin E2 in blood serum and to verify that its changes after surgical treatment are associated with the decrease of gastroesophageal reflux and esophageal inflammation. Materials and methods. 35 patients were examined with GERD who underwent laparoscopic total antireflux fundoplication. There were 26 women (74.3 %) and 9 men (25.7 %). Their age is 55.3 ± 11.3. The control group consisted of 20 practically healthy people (women – 14 (70.0 %), men – 6 (30.0 %), average age – 56.7 ± 10.6). Immunoenzymatic analysis of prostaglandin E2 was performed in blood plasma, which was obtained according to a standard method. Determination of prostaglandin E2 (Prostaglandin E2 ELISA, KGE004B, RnD Systems) was carried out by the immunoenzymatic method based on the use of the “sandwich“ variant of the solid-phase immunoenzymatic analysis. The procedure was carried out on the immunoenzyme complex ImmunoChem-2100 (USA) at the Department of Clinical Laboratory Diagnostics in Zaporizhzhia State Medical University. Research on the level of prostaglandin E2 in the main group was carried out before surgical treatment and 2–3 months after surgery by taking venous blood and using the above test systems. Statistical evaluation of the research results was performed using the Statistica for Windows 13 software package (StatSoft Inc., No. JPZ804I382130ARCN10-J). Results. The level of prostaglandin E2 in the blood of practically healthy people was 16.7 ± 6.1 pg/ml. In the main group, the values of prostaglandin E2 before surgical treatment were 25.8 ± 5.7 pg/ml, after surgical treatment, they decreased to 13.5 ± 5.3 pg/ml. The detailed analysis of patients in the main group showed that the level of prostaglandin E2 did not differ statistically in different erosive forms of esophagitis, or CLE and NERD. But it is statistically different from the level of prostaglandin E2 in practically healthy individuals of the control group. The conducted correlation analysis indicated that the level of prostaglandin E2 did not depend on the duration of acid exposure in the esophagus, as well as on the severity of esophagitis or the presence of CLE. Conclusions. With effective surgical treatment of gastroesophageal reflux disease, a decrease in the level of prostaglandin E2 after surgery is determined compared to preoperative data to the level obtained in a group of practically healthy patients. The obtained dynamics of the level of prostaglandin E2 indicates the possibility of this hormone influencing the tone of the lower esophageal sphincter and active participation in the pathogenesis of GERD, which confirms the possibility of its use as an additional diagnostic marker of inflammation in the esophagus and a marker of the effectiveness of surgical treatment.

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Section: Discussionmentioning

confidence: 99%

Dynamics of prostaglandin E2 in the surgical treatment of gastroesophageal reflux disease

Haidarzhi¹

2023

Patologìâ

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“…Along with this, in-depth studies on the etiological and pathogenetic factors in the development of GERD are being conducted everywhere, the results of which will help solve some problematic issues in achieving a stable positive therapeutic effect [9,10,11]. One of particular interest is the role of humoral influence on the GERD pathogenesis and the possibility of using the obtained data to improve the results of both medical and surgical treatment [12,13].…”

Section: ключові словаmentioning

confidence: 99%

“…Today, a number of hormones are known to regulate the functioning of the upper gastrointestinal tract muscles and also affect both the contraction and relaxation of the lower esophageal sphincter, the dysfunction of which plays one of the decisive roles in the development of GERD [14,15,16,17]. Among them, a special place is occupied by vasoactive intestinal peptide (VIP), which is one of the important neurotransmitters and neuromodulator that inhibits the reaction of the lower esophageal sphincter (LES) to various stimuli and, thus, contributes to its relaxation [13,18,19,20].…”

Section: ключові словаmentioning

confidence: 99%

Vasoactive intestinal polypeptide level in gastroesophageal reflux disease before and after surgical treatment

Haidarzhi¹,

Holovko²,

Okhrimenko³

2023

ZMJ

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From year to year, wide spread of gastroesophageal reflux disease (GERD) is attracting greater attention of specialists in the field of this pathology diagnosis and treatment. In-depth studies on etiological and pathogenetic factors in the GERD development are being conducted. Of particular interest is the role of humoral factors, one of which is vasoactive intestinal peptide (VIP). There are currently no data on the dynamics of this hormone during surgical treatment of GERD. The aim of the study was to examine the dynamics of plasma VIP during surgical treatment of GERD and its influence on the lower esophageal sphincter (LES) function. Materials and methods. Surgical treatment in the Nissen modification was performed for 35 patients with GERD. There were 26 women (74.3 %), men – 9 (25.7 %). mean age – 55.3 ± 11.3 years. Comparison group – 20 apparently healthy individuals: women – 14 (70.0 %); men – 6 (30.0 %), mean age – 56.7 ± 10.6 years. VIP was measured in venous blood plasma by an enzyme immunoassay (Vasoactive intestinal peptide ELISA, S-1201, BCM Diagnostics) using an immunoenzyme complex ImmunoChem-2100 (USA). The sampling of the studied samples in the main group was carried out before the operation and 2–3 months post-surgery. Results. In the main group before the surgery, the level of VIP was 3.1 ± 1.1 ng/ml, after the surgery – 2.2 ± 1.0 ng/ml. In the comparison group, VIP was determined at the level of 2.1 ± 1.1 ng/ml. In GERD before the surgery, the VIP level was statistically different from the indicators in the comparison group and from postoperative values. After surgical treatment, VIP values were decreased to the level of apparently healthy individuals. There was a positive correlation between the VIP level and acid exposure time (AET), the total number of refluxes, the number of reflux events longer than 5 minutes, the maximum duration of refluxes and the degree of esophageal inflammation. Conclusions. In the surgical treatment of GERD, the statistically significant decrease in the VIP level to that of apparently healthy individuals is determined. The moderate positive correlation between the levels of VIP, AET, the average number of refluxes, the number of reflux events longer than 5 minutes, and the maximum duration of refluxes confirms the inhibitory effect of VIP on LES tone. The relationship between the degree of esophageal inflammation and the VIP level confirms the indirect, through the action of VIP, inhibitory effect of the esophagitis severity on the LES tone.

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Barrier effect and wound healing activity of the medical device REF-FTP78 in the treatment of gastroesophageal reflux disease

Pecora¹,

Parisi

Bertin³

et al. 2022

Sci Rep

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REF-FTP78 is a class IIb medical device present on the market with different trade names and developed for the treatment of gastroesophageal reflux disease (GERD). This medical device is based on polysaccharides from Aloe Barbadensis and fucoidans from brown seaweeds, such as Undaria pinnatifida and Fucus vesiculosus, and aims to exert a protective effect on the esophageal mucosa against the noxious components of refluxate. The present study reports on the efficacy of REF-FTP78 devoting a particular attention to the barrier effect and wound healing properties, combined with antioxidant and anti-inflammatory activities. Film-forming properties and barrier effect were investigated on in vitro reconstructed human esophageal epithelium, through TEER measurement and evaluation of caffeine and Lucifer yellow permeability, and in an ex vivo swine model of esophageal mucosa damage. Antioxidant and anti-inflammatory properties were evaluated in terms of scavenging activity towards DPPH, ABTS and NO radicals and a wound healing assay was carried out to study the influence of the product on cell migration. The obtained results highlighted a significant barrier effect, with a reduction in caffeine penetration equal to 65.3%, the ability to both repair and prevent the damage caused by an acid insult, confirmed by a good transepithelial resistance for the tissue treated with the tested item, and the capacity to promote wound healing. Furthermore, the tested product showed good antioxidant and anti-inflammatory properties in the performed radical scavenging assays. These findings support the use of REF-FTP78 in the treatment of GERD.

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Weak acids induce PGE2 production in human oesophageal cells: novel mechanisms underlying GERD symptoms

Cited by 5 publications

References 47 publications

Dynamics of prostaglandin E2 in the surgical treatment of gastroesophageal reflux disease

Dynamics of prostaglandin E2 in the surgical treatment of gastroesophageal reflux disease

Vasoactive intestinal polypeptide level in gastroesophageal reflux disease before and after surgical treatment

Barrier effect and wound healing activity of the medical device REF-FTP78 in the treatment of gastroesophageal reflux disease

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