Weak biophoton emission after laser surgery application in soft tissues: Analysis of the optical features

Boschi, Federico; Basso, Petra Rita; Corridori, Ilaria; Durando, Giovanni; Sandri, Angela; Segalla, Gabriel; Raspanti, Mario; Spinelli, Antonello E.

doi:10.1002/jbio.201800260

Cited by 9 publications

(8 citation statements)

References 19 publications

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“…We previously reported that high‐intensity focused ultrasound or direct heating leads to a weak light emission from soft tissues [25]. Moreover, we verified that in laser‐induced artificial lesions on soft tissues the light emission is due to heat rather than to light stimulation [20]. Thus, it is reliable that light emission is generated by heat also in the bone.…”

Section: Discussionsupporting

confidence: 65%

“…Using an 810 nm diode laser on the oral mucosa of fresh pig mandibles, Goharkhay et al observed that the depth of the epithelial damage depended on laser penetration and correlated strongly with average powers used [19]. Recently, we demonstrated that a surgical diode laser operating at 808 nm with power in 3 to 9 W range is suitable to evaluate thermoluminescence emission from soft tissues [20]. Using the same laser, in this study we aim at elucidating the thermo‐optical features of laser‐irradiated bone tissue.…”

Section: Introductionmentioning

confidence: 99%

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Photon emission and changes in fluorescent properties of bone after laser irradiation

Sandri

Basso

Corridori

et al. 2021

Journal of Biophotonics

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Laser scalpels used in medical surgery concentrate light energy, heating the tissues. Recently, we reported thermoluminescence emission from laser‐treated soft tissues. Here we investigated the thermo‐optical effects caused by a laser operating at 808 nm on animal bones (beef ribs) through luminescence and fluorescence imaging, thermal imaging and scanning electron microscopy. Laser‐induced artificial lesions emitted luminescence peaking around 650 nm, with a half‐life of almost 1 hour. As concerns fluorescence, 24 hours after laser treatment we observed an increase of the emission and a shift from 500 (untreated) to 580 nm (treated). Recrystallization observed by SEM indicates that the temperature in the artificial lesions is over 600°C. We can conclude that laser treatment induces specific luminescent and fluorescent emissions due to heating of the bone and modification of its components. Monitoring these emissions could help prevent tissue overheating and its potential damages during laser‐assisted medical procedures.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Photon emission and changes in fluorescent properties of bone after laser irradiation

Sandri

Basso

Corridori

et al. 2021

Journal of Biophotonics

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“…These results also suggest us that surgery and CLI should not be performed much later than 4 h after injection, in order for CLI to able to detect surgical margins. Another possible complication is light emission by soft tissues due to the laser excision of the specimen [15]. This phenomenon has been observed both in the breast and in the prostate, thus we expect a similar behavior also for the liver.…”

Section: Discussionmentioning

confidence: 56%

In-vitro Study for Cerenkov Luminescence Imaging Application in Hepatic Metastasectomy

et al. 2021

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Cerenkov luminescence imaging (CLI) has been recently proposed as a method to visualize surgical margins in the operating theater, immediately after resection, to allow refining surgery in a single procedure. Our group is preparing a pilot clinical study to evaluate the impact of CLI during hepatic metastasectomy, using 18F-FDG and 68Ga-DOTATOC. Currently, we are optimizing the clinical protocol in terms of patient inclusion criteria, activity to inject, maximum allowed delay for imaging, and radiation monitoring. This paper describes a preliminary study we have performed to define the clinical protocol. The study is composed of two branches: 1) an in-vitro study to predict the typical signals and optical attenuation in the liver with 18F and 68Ga, 2) an analysis of clinical PET/CT data to determine typical values of relevant parameters, such as uptake and lesion dimension. The combined information by these two branches gives us an indication of the feasibility of CLI for margin assessment in liver metastasectomy. For 68Ga, we obtained detection limits ranging from 0.55 to 3.5 kBq/cc, to be compared with minimum and mean clinical uptakes of 1.6 and 7 kBq/cc, respectively. For 18F, the detection limits ranged from 12 to 145 kBq/cc, and the minimum and mean clinical uptakes were 5 and 11 kBq/cc, respectively. From these values, we expect CLI with 68Ga to be able to detect surgical margins in most patients, while with 18F the activities to inject for sufficient signal-to-noise ratio should be larger than standards, or the time delay between injection and imaging largely reduced. The results reported here can be useful also more in general, for studies dedicated to other CLI applications in the liver.

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“…The higher the level of an organism is, the greater intensity of the photon radiation that is emitted. The research applying of biophoton technology has been mainly conducted in medical fields, such as medical information diagnosis [11], cancer classification [12], analysis of brain activities [13], and others. In the cereal storage field, however, biophoton studies mainly focus on insect intrusion rather than on wheat mould.…”

Section: Fig 1 Global Consumption Of Wheat and Year-on-year Percentmentioning

confidence: 99%

A Fast Detection Method for Wheat Mould Based on Biophotons

Gong

Liang

et al. 2020

Preprint

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Mould is a common phenomenon in stored wheat. First, mould will decrease the quality of wheat kernels. Second, the mycotoxins metabolized by mycetes are very harmful for humans. Therefore, the fast and accurate examination of wheat mould is vitally important to evaluating its storage quality and subsequent processing safety. Existing methods for examining wheat mould mainly rely on chemical methods, which always involve complex and long pretreatment processes, and the auxiliary chemical materials used in these methods may pollute our environment. To improve the determination of wheat mould, this paper proposed a type of green and nondestructive determination method based on biophotons. The specific implementation process is as follows: first, the ultra-weak luminescence between healthy and mouldy wheat samples are measured repeatedly by a biophotonic analyser, and then, the approximate entropy and multiscale approximate entropy are separately introduced as the main classification features. Finally, the classification performances have been tested using the support vector machine(SVM). The ROC curve of the newly established classification model shows that the highest recognition rate can reach 93.6%, which shows that our proposed classification model is feasible and promising for detecting wheat mould.

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Weak biophoton emission after laser surgery application in soft tissues: Analysis of the optical features

Cited by 9 publications

References 19 publications

Photon emission and changes in fluorescent properties of bone after laser irradiation

Photon emission and changes in fluorescent properties of bone after laser irradiation

In-vitro Study for Cerenkov Luminescence Imaging Application in Hepatic Metastasectomy

A Fast Detection Method for Wheat Mould Based on Biophotons

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