2012
DOI: 10.1371/journal.pone.0051222
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Weak Glycolipid Binding of a Microdomain-Tracer Peptide Correlates with Aggregation and Slow Diffusion on Cell Membranes

Abstract: Organized assembly or aggregation of sphingolipid-binding ligands, such as certain toxins and pathogens, has been suggested to increase binding affinity of the ligand to the cell membrane and cause membrane reorganization or distortion. Here we show that the diffusion behavior of the fluorescently tagged sphingolipid-interacting peptide probe SBD (Sphingolipid Binding Domain) is altered by modifications in the construction of the peptide sequence that both result in a reduction in binding to ganglioside-contai… Show more

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Cited by 8 publications
(16 citation statements)
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“…Previously, SBD peptide was suggested to bind to gangliosides in the presence of sphingomyelin and cholesterol using surface plasmon resonance and fluorescence correlation spectroscopic assays [ 37 , 38 ]. GT1b was found to have a marginally higher binding at neutral pH than other common brain gangliosides, such as GM1, GD1a, and GQ1b [ 20 , 38 ]. Our simulations employed the same 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), sphingomyelin (18:0) (SM) and cholesterol (CHOL) composition used in these experimental studies.…”
Section: Resultsmentioning
confidence: 99%
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“…Previously, SBD peptide was suggested to bind to gangliosides in the presence of sphingomyelin and cholesterol using surface plasmon resonance and fluorescence correlation spectroscopic assays [ 37 , 38 ]. GT1b was found to have a marginally higher binding at neutral pH than other common brain gangliosides, such as GM1, GD1a, and GQ1b [ 20 , 38 ]. Our simulations employed the same 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), sphingomyelin (18:0) (SM) and cholesterol (CHOL) composition used in these experimental studies.…”
Section: Resultsmentioning
confidence: 99%
“…It has been proposed that toxin-membrane interactions are mediated by a discrete sphingolipid binding motif rich in aromatic amino acids containing one or more key basic residues [ 19 , 20 ]. This loosely-defined domain was suggested to exist in Aβ [ 21 ], HIV gp120 [ 22 ], prion [ 23 ], Shiga toxin [ 24 ], as well as more recently in α-synuclein [ 25 ].…”
Section: Introductionmentioning
confidence: 99%
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“…FCS has been also used to study membrane-induced aggregation of peptides [124,125] (number 3 in Fig. 1).…”
Section: Oligomerisation Studiesmentioning
confidence: 99%
“…Therefore the understanding of the behaviour of such living cells is a very important issue in bioelectronics. Several optical bio-compatible methods are known for the investigation of cellular behaviour in culture: infrared spectroscopy 3 , surface plasmon resonance 4 , optical coherence tomography 5 , and bioluminescence imaging 6 . In addition, there is progress in non-optical methods such as electron tomography 7 .…”
mentioning
confidence: 99%