Weak power frequency magnetic fields induce microtubule cytoskeleton reorganization depending on the epidermal growth factor receptor and the calcium related signaling

Abstract

We have shown previously that a weak 50 Hz magnetic field (MF) invoked the actin-cytoskeleton, and provoked cell migration at the cell level, probably through activating the epidermal growth factor receptor (EGFR) related motility pathways. However, whether the MF also affects the microtubule (MT)-cytoskeleton is still unknown. In this article, we continuously investigate the effects of 0.4 mT, 50 Hz MF on the MT, and try to understand if the MT effects are also associated with the EGFR pathway as the actin-cytoskeleton effects were. Our results strongly suggest that the MF effects are similar to that of EGF stimulation on the MT cytoskeleton, showing that 1) the MF suppressed MT in multiple cell types including PC12 and FL; 2) the MF promoted the clustering of the EGFR at the protein and the cell levels, in a similar way of that EGF did but with higher sensitivity to PD153035 inhibition, and triggered EGFR phosphorylation on sites of Y1173 and S1046/1047; 3) these effects were strongly depending on the Ca2+ signaling through the L-type calcium channel (LTCC) phosphorylation and elevation of the intracellular Ca2+ level. Strong associations were observed between EGFR and the Ca2+ signaling to regulate the MF-induced-reorganization of the cytoskeleton network, via phosphorylating the signaling proteins in the two pathways, including a significant MT protein, tau. These results strongly suggest that the MF activates the overall cytoskeleton in the absence of EGF, through a mechanism related to both the EGFR and the LTCC/Ca2+ signaling pathways.

Micro Magnetic Field Produced by Fe3O4 Nanoparticles in Bone Scaffold for Enhancing Cellular Activity

“…Uniform dispersion in the scaffold may show more contact surface area, thus providing more attachment sites for cell attachment [59]. However, the Fe3O4 nanoparticles are In addition, the magnetic field can also activate various signal pathways of the cell, and collaboratively mediate the signal communication between them, such as the classic mitogen-activated protein kinase [55][56][57] and BMP signal pathway [17,58], thereby promoting the expression of growth factors, improving the activity of runt-related transcription factor 2 and ALP, accelerating the growth and differentiation of osteoblasts, and promoting bone repair finally. Of course, the micro magnetic force generated in the microenvironment of the magnetic scaffold can provide continuous dynamic mechanical stimulation to MG63 cells, which can also improve the cells adhere and migrate.…”

Radical pairs may play a role in microtubule reorganization

“…Wang et al show that exposure to HMF caused tubulin assembly disorder 38 . Moreover, Wu et al observe that low-frequency sub-millitesla MF modulates the density of MTs in cells 39 . All these observations establish the magnetosensitivity of MTs for wide ranges of MF strengths.…”

“…In the following, we review the experimental results for the effects of applied magnetic field 38,39 on the density of MTs. We then describe the quantum spin dynamics of our radical pair model by a simple kinetic model for the MT dynamics.…”

Effect of electromagnetic radiation on the liver structure and ultrastructure of in utero irradiated rats

“…Our study also showed that EMR may cause marked changes in the shape and arrangement of microvilli of the hepatocytes. The changes observed in our experiment could be a consequence of a disturbance of the cytoskeleton, especially actin filaments or changes in the intracellular Ca 2+ concentration (Wu et al 2018).…”

“…EMR affects various processes that take place in the cells, such as synthesis of proteins and DNA transcription (Del Re et al 2019), transfer of ions through the cytoplasmic membrane and their subsequent accumulation in the cells (Lange 2000;Sun et al 2016), as well as changes in cytoskeleton organisation (Glade and Tabony 2005;Wu et al 2018).…”