The spinning rotor is a key element for rotor spinning, but fibers will wear when they move in the spinning rotor. In this paper, a model of fiber and spinning rotor was established to investigate the wearing properties of fibers and rotor wall influenced by stripping velocities, Young’s modulus and Poisson’s ratio. The simulation results indicate that the wear degree of fibers gradually increases with increasing stripping velocity, Young’s modulus and Poisson’s ratio. During the fibers’ movement in a rotor, there exists a stress concentration zone on processed fibers. The damage degree of the spinning rotor wall increases with stripping velocity and also varies depending on the differences in fiber properties. Taking the fibers from the stripping point as experimental samples, the surface wear of damaged fibers is observed by scanning electron microscopy, and the decrease in fiber strength with stripping velocity is measured. The experimental results show a good agreement with simulations. The research in this paper provides a preliminary guide for the selection of fiber raw materials and optimization of process parameters in rotor spinning, which in turn improves the physical properties of rotor yarn.