"It is challenging to experimentally determine the micro-friction mechanism of cotton fiber and metal in the sliding process. The influence of load and temperature on the interface behavior during dry friction between amorphous cotton fiber and chromium, the contact interface evolution and friction coefficient are studied using reactive molecular dynamics. The simulation results show that chromium–oxygen bonds are formed on the contact interface of the friction system during the sliding process. Furthermore, the relationship between friction coefficient, temperature, and load varies with the mechanical state of cotton cellulose. The relationship is positive when the cotton cellulose is in the glassy state. However, when cotton cellulose is in a highly elastic state, its friction coefficient is negatively related to the load. This study systematically evaluated the effects of temperature and load on the slip process from the atomic scale, provided a reason for the wear of the hard materials of the friction pair, and provided theoretical support for the study of this type of friction mechanism."