Estimation of vivo muscle forces during human motion is important for understanding human motion control mechanisms and joint mechanics. This paper combined the advantages of the convolutional neural network (CNN) and long-short-term memory (LSTM) and proposed a novel muscle force estimation method based on CNN–LSTM. A wearable sensor system was also developed to collect the angles and angular velocities of the hip, knee, and ankle joints in the sagittal plane during walking, and the collected kinematic data were used as the input for the neural network model. In this paper, the muscle forces calculated using OpenSim based on the Static Optimization (SO) method were used as the standard value to train the neural network model. Four lower limb muscles of the left leg, including gluteus maximus (GM), rectus femoris (RF), gastrocnemius (GAST), and soleus (SOL), were selected as the studying objects in this paper. The experiment results showed that compared to the standard CNN and the standard LSTM, the CNN–LSTM performed better in muscle forces estimation under slow (1.2 m/s), medium (1.5 m/s), and fast walking speeds (1.8 m/s). The average correlation coefficients between true and estimated values of four muscle forces under slow, medium, and fast walking speeds were 0.9801, 0.9829, and 0.9809, respectively. The average correlation coefficients had smaller fluctuations under different walking speeds, which indicated that the model had good robustness. The external testing experiment showed that the CNN–LSTM also had good generalization. The model performed well when the estimated object was not included in the training sample. This article proposed a convenient method for estimating muscle forces, which could provide theoretical assistance for the quantitative analysis of human motion and muscle injury. The method has established the relationship between joint kinematic signals and muscle forces during walking based on a neural network model; compared to the SO method to calculate muscle forces in OpenSim, it is more convenient and efficient in clinical analysis or engineering applications.
