25 Weatherization of residential homes is a popular retrofit procedure to improve the energy 26 efficiency of older homes by reducing building leakage. It is a vital tool in the fight against 27 climate change. Several studies have evaluated the effect of weatherization on indoor pollutants 28 such as formaldehyde and radon, but few studies have evaluated the effect of weatherization on 29 indoor particulates and microbial exposure. In this study, we compared the effect of change in 30 building leakage on indoor pollutants and bacterial communities in weatherized compared to 31 non-weatherized single-family residential homes in New York State. Nine weatherized and 32 eleven non-weatherized single-family homes in Tompkins County, New York were sampled 33 twice: before and after the weatherization procedures for case homes, and at least 3 months apart 34 for control homes that were not weatherized. We found a significant increase in both indoor-35 outdoor temperature ratio and living-area-and basement-radon levels of weatherized homes 36 compared to control homes. The indoor-outdoor relative humidity ratio significantly decreased in 37 weatherized compared to control homes. The indoor microbiome also became less similar to the 38 outdoor community after weatherization. Compared to the changes in ventilation rate, 39 temperature, relative humidity, and occupancy, the change in season was a more predictive 40 measure of indoor bacterial concentration. Ventilation rate reduction from weatherization 41 procedures led to an increase in indoor radon levels, as well as a warmer and less humid indoor 42 environment. However, it did not affect indoor particulate mass concentration or indoor airborne 43 bacteria load, and did only marginally affect the microbiome composition of residential homes.44 Finally, we found that changes in airborne bacterial load are more sensitive to shifts in season, 45 whereas radon levels are more sensitive to ventilation rate.3 47 Introduction 48 Today, twenty-two percent of the total U.S. energy consumption is used in residential buildings, 49 mainly to heat and cool homes (1). Since the energy crisis of the 1970s, national policy 50 discussions have been focused on energy efficiency and energy conservation (2). This led to the 51 establishment of building energy codes such as the 1975 ASHRAE Energy Code and the Energy 52 Policy and Conservation Act (EPCA) (3). Included in the EPCA was the creation of the 53 Weatherization Assistance Program (WAP) by the Department of Energy (4). Weatherization is 54 the practice of protecting a building from environmental fluctuations to reduce energy 55 consumption in the building. WAP provides financial assistance to low-income households in 56 single-family dwellings to weatherize their home through a combination of procedures including 57 insulation and fixture repairs (4, 5). Besides improving the household energy efficiency, 58 weatherization also provides an additional cost-saving incentive in regards to heating expenses 59 and improved thermal comfort...