Conventional imaging when evaluating the functional anatomy of the pelvic floor is limited to supine in spite of the known influence of changes in posture on urinary incontinence (UI). Pelvic floor muscle therapy (PFMT), the mainstay of treatment for UI, lacks measures of metabolic/physiologic function able to quantify treatment effect. And, while yoga has been found to be beneficial for UI symptom management, it is not currently clear what anatomically occurs to yield these benefits. Studies indicate that advanced imaging and spectroscopic techniques can now offer more comprehensive initial evaluation for UI, new insights into anatomic effects of yoga as therapy, and a novel physiologic measure of treatment effect. Upright open magnetic resonance imaging allows the impact of gravity on the pelvic organs to be visualized, also, the effects of posture, and how different yoga poses affect the urethra. Near-infrared spectroscopy of the pelvic floor muscles (PFM) provides an absolute measure of reoxygenation following sustained maximal voluntary contraction able to quantify PFM ‘fitness’ and document treatment effects. These entities offer improvements in the evaluation of pelvic floor dysfunction, insights into how yoga may alleviate UI, and a means whereby healthcare providers and patients can measure treatment effect occurring through PFMT.