Web hybrid chain reaction enhanced fluorescent magnetic bead array for digital nucleic acid detection

Jin, Furui; Liu, Min; Xu, Danke

doi:10.1016/j.talanta.2022.123968

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…Various aspects of microparticles have been utilized for HBV biosensing. For instance, the high surface area of carboxyl-functionalized magnetic beads was used to densely hybridize HBV DNA probes for an isothermal web hybridization chain reaction ( Figure 3 c) [ 82 ]. Strong fluorescent signals could be obtained from the amplified complexes on microparticles, achieving a detection limit of 5 fM HBV.…”

Section: Viruses Detected Using Microparticlesmentioning

confidence: 99%

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Microparticle-Based Detection of Viruses

Khanthaphixay

Yoon

2023

Biosensors

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Surveillance of viral pathogens in both point-of-care and clinical settings is imperative to preventing the widespread propagation of disease—undetected viral outbreaks can pose dire health risks on a large scale. Thus, portable, accessible, and reliable biosensors are necessary for proactive measures. Polymeric microparticles have recently gained popularity for their size, surface area, and versatility, which make them ideal biosensing tools. This review cataloged recent investigations on polymeric microparticle-based detection platforms across eight virus families. These microparticles were used as labels for detection (often with fluorescent microparticles) and for capturing viruses for isolation or purification (often with magnetic microparticles). We also categorized all methods by the characteristics, materials, conjugated receptors, and size of microparticles. Current approaches were compared, addressing strengths and weaknesses in the context of virus detection. In-depth analyses were conducted for each virus family, categorizing whether the polymeric microparticles were used as labels, for capturing, or both. We also summarized the types of receptors conjugated to polymeric microparticles for each virus family.

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Section: Viruses Detected Using Microparticlesmentioning

confidence: 99%

“…( c ) Fluorescent magnetic microparticle array for detecting hepatitis B virus. Reprinted with permission from [ 82 ]. DNA probes were coupled to the microparticle surface via EDC chemistry, facilitating the hybridization chain reaction (HCR) of fluorescent probes.…”

Section: Viruses Detected Using Microparticlesmentioning

confidence: 99%

Microparticle-Based Detection of Viruses

Khanthaphixay

Yoon

2023

Biosensors

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Direct Zeptomole Detection of RNA Biomarkers by Ultrabright Fluorescent Nanoparticles on Magnetic Beads

Cruz Da Silva,

Gaki,

Flieg

et al. 2024

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Nucleic acids are important biomarkers in cancer and viral diseases. However, their ultralow concentration in biological/clinical samples makes direct target detection challenging, because it leads to slow hybridization kinetics with the probe and its insufficient signal‐to‐noise ratio. Therefore, RNA target detection is done by molecular (target) amplification, notably by RT‐PCR, which is a tedious multistep method that includes nucleic acid extraction and reverse transcription. Here, a direct method based on ultrabright dye‐loaded polymeric nanoparticles in a sandwich‐like hybridization assay with magnetic beads is reported. The ultrabright DNA‐functionalized nanoparticle, equivalent to ≈10 000 strongly emissive rhodamine dyes, is hybridized with the magnetic bead to the RNA target, providing the signal amplification for the detection. This concept (magneto‐fluorescent sandwich) enables high‐throughput detection of DNA and RNA sequences of varied lengths from 48 to 1362 nt with the limit of detection down to 0.3 fm using a plate reader (15 zeptomoles), among the best reported for optical sandwich assays. Moreover, it allows semi‐quantitative detection of SARS‐CoV‐2 viral RNA directly in clinical samples without a dedicated RNA extraction step. The developed technology, combining ultrabright nanoparticles with magnetic beads, addresses fundamental challenges in RNA detection; it is expected to accelerate molecular diagnostics of diseases.

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