Web Services Based Platform for the Cell Counting Problem

Castillo, Juan Carlos Jiménez del; Almeida, Francisco; Blanco, Vicente; Ramírez, Maurier

doi:10.1007/978-3-319-14325-5_8

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…The software tool allows a remote automatic processing of the cell images using a Web interface. To achieve this facility, we have integrated the cell image‐processing software, LLCECO (38) in a web services platform. LLCECO is a classification and cell counting software joint developed by High Performance Computing Group, Universidad de La Laguna and Stem Cell Molecular and Cellular Laboratory, University of Castilla La‐Mancha.…”

Section: Methodsmentioning

confidence: 99%

Defects in subventricular zone pigmented epithelium‐derived factor niche signaling in the senescence‐accelerated mouse prone‐8

et al. 2015

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We studied potential changes in the subventricular zone (SVZ) stem cell niche of the senescence-accelerated mouse prone-8 (SAM-P8) aging model. Bromodeoxyuridine (BrdU) assays with longtime survival revealed a lower number of label-retaining stem cells in the SAM-P8 SVZ compared with the SAM-Resistant 1 (SAM-R1) control strain. We also found that in SAM-P8 niche signaling is attenuated and the stem cell pool is less responsive to the self-renewal niche factor pigmented epithelium-derived factor (PEDF). Protein analysis demonstrated stable amounts of the PEDF ligand in the SAM-P8 SVZ niche; however, SAM-P8 stem cells present a significant expression decrease of patatin-like phospholipase domain containing 2, a receptor for PEDF (PNPLA2-PEDF) receptor, but not of laminin receptor (LR), a receptor for PEDF (LR-PEDF) receptor. We observed changes in self-renewal related genes (hairy and enhancer of split 1 (Hes1), hairy and enhancer of split 1 (Hes5), Sox2] and report that although these genes are down-regulated in SAM-P8, differentiation genes (Pax6) are up-regulated and neurogenesis is increased. Finally, sheltering mammalian telomere complexes might be also involved given a down-regulation of telomeric repeat binding factor 1 (Terf1) expression was observed in SAM-P8 at young age periods. Differences between these 2 models, SAM-P8 and SAM-R1 controls, have been previously detected at more advanced ages. We now describe alterations in the PEDF signaling pathway and stem cell self-renewal at a very young age, which could be involved in the premature senescence observed in the SAM-P8 model.

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Section: Methodsmentioning

confidence: 99%

Defects in subventricular zone pigmented epithelium‐derived factor niche signaling in the senescence‐accelerated mouse prone‐8

et al. 2015

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Spiral-Based Model for Software Architecture in Bio-image Analysis: A Case Study in RSV Cell Infection

Gamarra

Zurek

Bernal

et al. 2020

Computer Information Systems and Industrial Management

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The advancement in biological and medical image acquisitions has allowed the development of numerous investigations in different fields supported by image analysis, from cell to physiological level. The complexity in the treatment of data, generated by image analysis, requires a structured methodology for software development. In this paper we proposed a framework to develop a software solution with a Service-Oriented Architecture (SOA) applied to the analysis of biological images. The framework is completed with a novel image analysis methodology that would help researchers to achieve better results in their image analysis projects. We evaluate our proposal in a scientific project related to cell image analysis. Palabras clave Spiral methodology, Bio-image informatics, Cell image processing, Respiratory Syncytial Virus.

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A Service-Oriented Architecture for Bioinformatics: An Application in Cell Image Analysis

Gamarra

Zurek²,

Bernal³

et al. 2017

Advances in Intelligent Systems and Computing

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Web Services Based Platform for the Cell Counting Problem

Cited by 3 publications

References 7 publications

Defects in subventricular zone pigmented epithelium‐derived factor niche signaling in the senescence‐accelerated mouse prone‐8

Defects in subventricular zone pigmented epithelium‐derived factor niche signaling in the senescence‐accelerated mouse prone‐8

Spiral-Based Model for Software Architecture in Bio-image Analysis: A Case Study in RSV Cell Infection

A Service-Oriented Architecture for Bioinformatics: An Application in Cell Image Analysis

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