Weibel-Palade bodies as a storage site of calcitonin gene-related peptide and endothelin-1 in blood vessels of the rat carotid body

Ozaka, Takatoshi; Doi, Yoshiaki; Kayashima, Kotaro; Fujimoto, Sunao

doi:10.1002/(sici)1097-0185(199703)247:3<388::aid-ar10>3.0.co;2-l

Cited by 63 publications

(34 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Weibel-Palade bodies are regulated secretory granules not only for vWf but also for EC proteins like vasoactive peptides 39 and t-PA (this report). In addition, transfection of EC with coagulation factor VIII has demonstrated that factor VIII can be stored in Weibel-Palade bodies in a vWfdependent fashion.…”

Section: Rosnoblet Et Al Immunolocalization Of T-pa In Endothelial Cellsmentioning

confidence: 86%

Storage of Tissue-Type Plasminogen Activator in Weibel-Palade Bodies of Human Endothelial Cells

Rosnoblet¹,

Vischer²,

Gerard³

et al. 1999

ATVB

View full text Add to dashboard Cite

Abstract-Tissue-type plasminogen activator (t-PA) is acutely released by endothelial cells. Although its endothelial storage compartment is still not well defined, t-PA release is often accompanied by release of von Willebrand factor (vWf), a protein stored in Weibel-Palade bodies. We investigated, therefore, whether t-PA is stored in these secretory organelles. Under basal culture conditions, a minority of human umbilical vein endothelial cells (HUVEC) exhibited immunofluorescent staining for t-PA, which was observed only in Weibel-Palade bodies. To increase t-PA expression, HUVEC were infected with a t-PA recombinant adenovirus (AdCMVt-PA issue-type plasminogen activator (t-PA) is a key enzyme for the removal of incipient thrombi in the vascular system, and recombinant t-PA is now widely used for the thrombolytic therapy of myocardial infarction. Endothelial cells (EC) are the main source for plasma t-PA. 1-4 Although a variety of stimuli such as venous occlusion, exercise, or injection of vasoactive substances are known to acutely increase plasma levels of t-PA, the precise mechanisms responsible for this increase are poorly defined. They may involve an acute release of t-PA from EC, variations in the rate of production of t-PA by EC, or changes in the clearance rate. 5,6 Several in vivo and ex vivo studies give clear evidence for the occurrence of acute release of t-PA. Experimental induction of disseminated intravascular coagulation in chimpanzees or baboons results in a 50-fold increase in t-PA plasma levels within a few minutes. 7,8 The rapidity and the magnitude of the increase in the t-PA concentration, as well as the rapid return to normal levels, are consistent only with a massive release of t-PA from storage pools. The findings that injection of vasoactive agents such as thrombin or calcium ionophore leads to an acute increase of t-PA in isolated vascular systems are also consistent with an endothelial t-PA storage pool. 9 -11

show abstract

Section: Rosnoblet Et Al Immunolocalization Of T-pa In Endothelial Cellsmentioning

confidence: 86%

Storage of Tissue-Type Plasminogen Activator in Weibel-Palade Bodies of Human Endothelial Cells

Rosnoblet¹,

Vischer²,

Gerard³

et al. 1999

ATVB

View full text Add to dashboard Cite

show abstract

“…Ca 2ϩ -activated or ATP-sensitive K ϩ channels may be also responsible for this mechanism (345). CGRP and endothelin are colocalized in endothelial cells, suggesting autoregulatory mechanisms for blood flow (611). Intravenous injection of CGRP increased vasodilation and skin temperature in rats in a dose-dependent manner.…”

Section: Cgrpmentioning

confidence: 99%

Neuronal Control of Skin Function: The Skin as a Neuroimmunoendocrine Organ

Roosterman¹,

Goerge

Schneider

et al. 2006

Physiological Reviews

556

521

View full text Add to dashboard Cite

This review focuses on the role of the peripheral nervous system in cutaneous biology and disease. During the last few years, a modern concept of an interactive network between cutaneous nerves, the neuroendocrine axis, and the immune system has been established. We learned that neurocutaneous interactions influence a variety of physiological and pathophysiological functions, including cell growth, immunity, inflammation, pruritus, and wound healing. This interaction is mediated by primary afferent as well as autonomic nerves, which release neuromediators and activate specific receptors on many target cells in the skin. A dense network of sensory nerves releases neuropeptides, thereby modulating inflammation, cell growth, and the immune responses in the skin. Neurotrophic factors, in addition to regulating nerve growth, participate in many properties of skin function. The skin expresses a variety of neurohormone receptors coupled to heterotrimeric G proteins that are tightly involved in skin homeostasis and inflammation. This neurohormone-receptor interaction is modulated by endopeptidases, which are able to terminate neuropeptide-induced inflammatory or immune responses. Neuronal proteinase-activated receptors or transient receptor potential ion channels are recently described receptors that may have been important in regulating neurogenic inflammation, pain, and pruritus. Together, a close multidirectional interaction between neuromediators, high-affinity receptors, and regulatory proteases is critically involved to maintain tissue integrity and regulate inflammatory responses in the skin. A deeper understanding of cutaneous neuroimmunoendocrinology may help to develop new strategies for the treatment of several skin diseases.

show abstract

“…The secretory vesicles of the endothelium, known as Weibel-Palade bodies (WPB), were originally discovered as the storage depot of von Willebrand factor (vWF) (Weibel and Palade, 1964). Since their initial discovery via electron microscopy, WPBs have also been found to store and release P-selectin (Bonfanti et al, 1989;McEver et al, 1989), interleukin-8 (Utgaard et al, 1998;Wolff et al, 1998), endothelin-1 (Ozaka et al, 1997;Russell et al, 1998), angiopoietin-2 (Fiedler et al, 2004), tissue-type plasminogen activator (Huber et al, 2002), and other proteins that play important roles in the signaling pathways of inflammation, hemostasis, and tissue repair (for review, see Rondaij et al, 2006b;Valentijn et al, 2011).…”

Section: Vesicular Communication: the Exocytosis Microdomainmentioning

confidence: 99%

Regulation of Cellular Communication by Signaling Microdomains in the Blood Vessel Wall

et al. 2014

View full text Add to dashboard Cite

Weibel-Palade bodies as a storage site of calcitonin gene-related peptide and endothelin-1 in blood vessels of the rat carotid body

Cited by 63 publications

References 26 publications

Storage of Tissue-Type Plasminogen Activator in Weibel-Palade Bodies of Human Endothelial Cells

Storage of Tissue-Type Plasminogen Activator in Weibel-Palade Bodies of Human Endothelial Cells

Neuronal Control of Skin Function: The Skin as a Neuroimmunoendocrine Organ

Regulation of Cellular Communication by Signaling Microdomains in the Blood Vessel Wall

Contact Info

Product

Resources

About