Weissella sp. outbreaks in commercial rainbow trout (Oncorhynchus mykiss) farms in Brazil

Figueiredo, Henrique César Pereira; Costa, F A. A.; Leal, Carlos Augusto Gomes; Carvalho-Castro, G.A.; Leite, R. C.

doi:10.1016/j.vetmic.2011.11.008

Cited by 47 publications

(54 citation statements)

References 27 publications

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“…; Figueiredo et al . ), yet the Brazilian trout strain ( W. ceti WS08) genome encodes a homologue of locus WCNC_01820, and we predict identical amplicon sequences will be produced (Figueiredo et al . ).…”

Section: Resultsmentioning

confidence: 80%

Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout

Snyder

Hinshaw

Welch

2015

Letters in Applied Microbiology

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Weissellosis of rainbow trout is caused by the Gram-positive bacteria Weissella ceti and has been reported in China, Brazil and the United States. This disease can result in high mortality in market-sized fish and thus can cause significant economic loss. Thus far, phenotypic characterization and 16S rRNA sequencing have been used to confirm a Weissellosis diagnosis. Here, we present the development of PCR-based diagnostic tools for the rapid identification and quantification of W. ceti within bacteriological culture and infected tissues. A duplex PCR, which amplifies both genus- and strain-specific targets, positively identifies isolates as W. ceti NC36. A qPCR assay was also developed to quantify pathogen load from infected tissues, using a W. ceti NC36 unique locus. A proof of concept study was performed to demonstrate that quantification using traditional plate count methods and qPCR were significantly correlated when assessed from infected brain and spleen tissue. These tools were also used to confirm diagnosis of Weissellosis in a commercial rainbow trout farm during an outbreak investigation. These are the first diagnostic tools developed for identification and quantification of W. ceti infection within rainbow trout, contributing to rapid Weissellosis diagnosis, enhanced pathogen surveillance and epidemiological studies.

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Section: Resultsmentioning

confidence: 80%

Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout

Snyder

Hinshaw

Welch

2015

Letters in Applied Microbiology

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“…; Figuereido et al . ). However, as this and other studies show, there is not enough variation in the nucleotides sequences of the 16S rRNA gene of Fl.…”

Section: Discussionmentioning

confidence: 97%

Genetic variation amongFlavobacterium psychrophilumisolates from wild and farmed salmonids in Norway and Chile

et al. 2013

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Aims: To aim of the study was to describe the genetic relationship between isolates of Flavobacterium psychrophilum with a main emphasis of samples from Chile and Norway. The isolates have been obtained from farmed salmonids in Norway and Chile, and from wild salmonids in Norway, but isolates from North America and European countries are also included in the analysis. Methods and Results:The study is based on phylogenetic analysis of 16S rRNA and seven housekeeping genes (HG), gyrB, atpA, dnaK, trpB, fumC, murG and tuf, and the use of a multilocus sequence typing (MLST) system, based on nucleotide polymorphism in the HG, as an alternative to the phylogenies. The variation within the selected genes was limited, and the phylogenetic analysis gave little resolution between the isolates. The MLST gave a much better resolution resulting in 53 sequence types where the same sequences types could be found in Chile, North America and European countries, and in different host species. Conclusions: Multilocus sequence typing give a relatively good separation of different isolates of Fl. psychrophilum and show that there are no distinct geographical or host-specific isolates in the studied material from Chile, North America and Europe. Nor was it possible to separate between isolates from ulcers and systemic infections vs isolates from the surface of healthy salmonids. Significance and Impact of the Study: This study shows a wide geographical distribution of Fl. psychrophilum, indicating that the bacterium has a large potential for transmission over long distances, and between different salmonid hosts species. This knowledge will be important for future management of salmonids diseases connected to Fl. psychrophilum.

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“…Novel Weissella sp. bacteria have recently been associated with disease outbreaks in rainbow trout ( Oncorhynchus mykiss ) in China (7), Brazil (8), and the United States (T. J. Welch and C. M. Good, submitted for publication). Each of these outbreaks occurred at commercial rainbow trout farms and caused high levels of morbidity and mortality.…”

Section: Genome Announcementmentioning

confidence: 99%

Genome Sequence of Weissella ceti NC36, an Emerging Pathogen of Farmed Rainbow Trout in the United States

et al. 2013

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Weissella sp. outbreaks in commercial rainbow trout (Oncorhynchus mykiss) farms in Brazil

Cited by 47 publications

References 27 publications

Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout

Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout

Genetic variation amongFlavobacterium psychrophilumisolates from wild and farmed salmonids in Norway and Chile

Genome Sequence of Weissella ceti NC36, an Emerging Pathogen of Farmed Rainbow Trout in the United States

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