Weiterentwicklung einer Methode zur Bienenbruthaltung unter Laborbedingungen

Lüken, Dorothee; Janke, Martina; Lienau, Friedrich-Wilhelm; Ohe, Werner von der; Forster, Rolf

doi:10.1007/s00003-012-0759-y

Cited by 6 publications

(4 citation statements)

References 2 publications

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“…The in vivo therapeutic effect of phage HB10c2 on P. larvae ERIC I-and II-infected bee larvae was analyzed in breeding assays as described previously (19,40). Larvae Ͻ24 h old were transferred to 20 l of diet A (50% royal jelly and 50% aqueous solution containing 2% yeast extract, 12% glucose, and 12% fructose) (day 1).…”

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confidence: 99%

“…On day 3, the larvae were fed 20 l of diet B (50% royal jelly and 50% aqueous solution containing 3% yeast extract, 15% glucose, and 15% fructose). Increasing amounts (30,40, and 50 l) of diet C (50% royal jelly and 50% aqueous solution containing 4% yeast extract, 18% glucose, and 18% fructose) were fed from day 4 to day 6, respectively. Three replicates of 48 larvae from different, nonrelated queens were used for infection.…”

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confidence: 99%

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Paenibacilluslarvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae

Beims

Wittmann

Bunk

et al. 2015

Appl Environ Microbiol

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dPaenibacillus larvae is the causative agent of American foulbrood (AFB), the most serious honey bee brood bacterial disease. We isolated and characterized P. larvae-directed bacteriophages and developed criteria for safe phage therapy. Whole-genome analysis of a highly lytic virus of the family Siphoviridae (HB10c2) provided a detailed safety profile and uncovered its lysogenic nature and a putative beta-lactamase-like protein. To rate its antagonistic activity against the pathogens targeted and to specify potentially harmful effects on the bee population and the environment, P. larvae genotypes ERIC I to IV, representatives of the bee gut microbiota, and a broad panel of members of the order Bacillales were analyzed for phage HB10c2-induced lysis. Breeding assays with infected bee larvae revealed that the in vitro phage activity observed was not predictive of the real-life scenario and therapeutic efficacy. On the basis of the disclosed P. larvae-bacteriophage coevolution, we discuss the future prospects of AFB phage therapy.

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confidence: 99%

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confidence: 99%

Paenibacilluslarvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae

Beims

Wittmann

Bunk

et al. 2015

Appl Environ Microbiol

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“…Carniolan honey bee, A. mellifera carnica , L1 larvae <30 h old were obtained from hives at the Technische Universität Braunschweig (Braunschweig, Germany) using queen bees that were bred at the Lower Saxony State Office for Consumer Protection and Food Safety, Institute for Apiculture (Celle, Germany) and reared as described by Lüken et al (2012). The purified Trc mixture (without gramicidins) was prepared from commercial Tcn (Sigma-Aldrich, Steinheim, Germany) as described by Hotchkiss and Dubos (1941).…”

Section: Oral Tolerance Of Bee Larvae Toward Tcn and Trc Mixturementioning

confidence: 99%

“…Rearing of bee larvae was performed as previously described (Beims et al 2015;Lüken et al 2012). At day 1, 500 spores of P. larvae ERIC I (DSM 7030) or ERIC II (DSM 25430) were added to Diet A (OECD 2013) to infect the larvae (Beims et al 2015), barring those which served as uninfected controls.…”

Section: Curing Assays Of Paenibacillus Larvaeinfected Bee Larvaementioning

confidence: 99%

Tolerance of honey bee adults and larvae toward tyrothricin peptides derived from Brevibacillus parabrevis

et al. 2017

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Tyrothricin is a peptide complex containing the linear gramicidins and cyclic tyrocidines. The tyrocidines have potent activity against fungal plant pathogens. As these peptides have possible agricultural applications, their toxicity was evaluated toward honey bee adults and larvae. Tyrothricin formulated in sucrose was non-toxic to caged adult honey bees at up to 1.5 g/L over 48-h exposure, which is 100-to 200-fold higher than the amount needed to eradicate high fungal loads (2 × 10 4 spores/mL). Moreover, tyrothricin and the tyrocidines displayed potent in vitro activity toward foulbrood causing pathogens (Paenibacillus larvae , Melissococcus plutonius , Paenibacillus alvei ) in honey bee larvae. In vivo tyrothricin or tyrocidine treatment delayed infection onset, indicating potential for curing. Tyrothricin was also found to be non-toxic with possible protective action in a semi-field trial on young bees released into hives, indicating the relative safety of the application of these antimicrobial peptides in an agricultural setting. tyrothricin / tyrocidine / antimicrobial peptides / agriculture / antifungal / honey bee tolerance Corresponding author: J. Arnold Vosloo, arnie@sun.ac.za; M. Rautenbach, mra@sun.ac.za

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Paenilarvins: Iturin Family Lipopeptides from the Honey Bee Pathogen Paenibacillus larvae

et al. 2014

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The bacterium Paenibacillus larvae has been extensively studied as it is an appalling honey bee pathogen. In the present work, we screened crude extracts derived from fermentations of P. larvae genotypes ERIC I and II for antimicrobial activity, following the detection of four putative secondary metabolite gene clusters that show high sequence homology to known biosynthetic gene clusters for the biosynthesis of antibiotics. Low molecular weight metabolites produced by P. larvae have recently been shown to have toxic effects on honey bee larvae. Moreover, a novel tripeptide, sevadicin, was recently characterized from laboratory cultures of P. larvae. In this study, paenilarvins, which are iturinic lipopeptides exhibiting strong antifungal activities, were obtained by bioassay-guided fractionation from cultures of P. larvae, genotype ERIC II. Their molecular structures were determined by extensive 2D NMR spectroscopy, high resolution mass spectrometry, and other methods. Paenilarvins are the first antifungal secondary metabolites to be identified from P. larvae. In preliminary experiments, these lipopeptides also affected honey bee larvae and might thus play a role in P. larvae survival and pathogenesis. However, further studies are needed to investigate their function.

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Weiterentwicklung einer Methode zur Bienenbruthaltung unter Laborbedingungen

Cited by 6 publications

References 2 publications

Paenibacilluslarvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae

Paenibacilluslarvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae

Tolerance of honey bee adults and larvae toward tyrothricin peptides derived from Brevibacillus parabrevis

Paenilarvins: Iturin Family Lipopeptides from the Honey Bee Pathogen Paenibacillus larvae

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