Well-Ordered Mesoporous Silica Nanoparticles as Cell Markers

Lin, Yu-Shen; Tsai, Chun-An; Huang, Hsing-Yi; Kuo, Ching‐Hua; Hung, Yu-Chueh; Huang, Dong-Ming; Chen, ‡ and Yao-Chang; Mou, Chung‐Yuan

doi:10.1021/cm051014c

Cited by 420 publications

(185 citation statements)

References 29 publications

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“…Several previous studies have shown that MSNs are able to undergo cellular uptake by endocytosis in various cancer cells without inducing cytotoxicity. The nanoparticles are mainly located in the acidic organelles such as endosomes and lysosomes when taken up by cells (11,12,(26)(27)(28)(29)(30)(31)(32). Our results are consistent with previous studies.…”

Section: Msn-hydrazone-dox Is Uptaken and Induces Apoptosis Of Tumor supporting

confidence: 93%

“…Recently, MSNs have been used in numerous biological fields, such as fluorescent markers in vitro and in vivo, clinical diagnoses, drug and gene delivery, and MRI contrast agents (12). In previously reported work, we developed the trifunctionalized MSNs for use as theranostic compounds that orchestrate the triad of imaging, target, and therapy in a single particle.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Chemotherapy of Cancer Using pH-Sensitive Mesoporous Silica Nanoparticles to Antagonize P-Glycoprotein–Mediated Drug Resistance

Huang

Sun

Cheng

et al. 2011

Molecular Cancer Therapeutics

109

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Multidrug resistance (MDR) is the major clinical obstacle in the management of cancer by chemotherapy. Overexpression of ATP-dependent efflux transporter P-glycoprotein (PGP) is a key factor contributing to multidrug resistance of cancer cells. The purpose of the present study was to use the endosomal pH-sensitive MSN (mesoporous silica nanoparticles; MSN-Hydrazone-Dox) for controlled release of doxorubicin (Dox) in an attempt to overcome the PGP-mediated MDR. In vitro cell culture studies indicate that uptake of MSNHydrazone-Dox by the human uterine sarcoma MES-SA/Dox-resistant tumor (MES-SA/Dx-5) cell occurs through endocytosis, thus bypassing the efflux pump resistance. This improves the efficacy of the drug and leads to significant cytotoxicity and DNA fragmentation evidenced by terminal deoxynucleotidyl transferasemediated dUTP nick end labeling and DNA laddering assays. In vivo studies show that the intratumor injection of MSN-Hydrazone-Dox induces significant apoptosis of MES-SA/Dox-resistant cancer cells. This is validated by active caspase-3 immunohistochemical analysis. However, MSN-Hydrazone, without doxorubicin conjugation, cannot induce apoptosis in vitro and in vivo. In conclusion, both in vitro and in vivo studies show that MSN could serve as an efficient nanocarrier entering cell avidly via endocytosis, thus bypassing the PGP efflux pump to compromise the PGP-mediated MDR. MSN-Hydrazone-Dox could further respond to endosomal acidic pH to release doxorubicin in a sustained manner. Besides the cell study, this is the first report that successfully shows the therapeutic efficacy of using MSN against MDR cancer in vivo. Mol Cancer Ther; 10(5); 761-9. Ó2011 AACR.

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Section: Msn-hydrazone-dox Is Uptaken and Induces Apoptosis Of Tumor supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Enhanced Chemotherapy of Cancer Using pH-Sensitive Mesoporous Silica Nanoparticles to Antagonize P-Glycoprotein–Mediated Drug Resistance

Huang

Sun

Cheng

et al. 2011

Molecular Cancer Therapeutics

109

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“…51 Briefly, FITC (10 μmol) was reacted with neat APTMS (200 μmol) overnight in the dark to yield the FITC-modified silane. 1 57 (d, aromatic).…”

Section: Synthesis Of Fluorescently-labeled No-releasing Silica Nanopmentioning

confidence: 99%

Bactericidal Efficacy of Nitric Oxide-Releasing Silica Nanoparticles

Hetrick

Shin²,

Stasko³

et al. 2008

ACS Nano

322

380

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The utility of nitric oxide (NO)-releasing silica nanoparticles as a novel antibacterial is demonstrated against Pseudomonas aeruginosa. Nitric oxide-releasing nanoparticles were prepared via co-condensation of tetraalkoxysilane with aminoalkoxysilane modified with diazeniumdiolate NO donors, allowing for the storage of large NO payloads. Comparison of the bactericidal efficacy of the NO-releasing nanoparticles to 1-[2-(carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolate (PROLI/NO), a small molecule NO donor, demonstrated enhanced bactericidal efficacy of nanoparticle-derived NO and reduced cytotoxicity to healthy cells (mammalian fibroblasts). Confocal microscopy revealed that fluorescently-labeled NO-releasing nanoparticles associated with the bacteria, providing rationale for the enhanced bactericidal efficacy of the nanoparticles. Intracellular NO concentrations were measurable when the NO was delivered from nanoparticles as opposed to PROLI/NO. Collectively, these results demonstrate the advantage of delivering NO via nanoparticles for antimicrobial applications.Keywords nitric oxide; silica nanoparticle; antibacterial; bactericidal; cytotoxicity; reactive nitrogen species; reactive oxygen species Antibiotic resistance has resulted in bacterial infections becoming the most common cause of infectious disease-related death. 1,2 In the United States alone, nearly 2 million people per year acquire infections during a hospital stay, of which approximately 90,000 die. 2 The primary culprits behind such deadly infections are antibiotic-resistant pathogens, which are responsible for approximately 70% of all lethal nosocomial infections. The growing danger of life-threatening infections and the rising economic burden of resistant bacteria have created a demand for new antibacterial therapeutics.The use of nanoparticles as delivery vehicles for bactericidal agents represents a new paradigm in the design of antibacterial therapeutics. To date, most antibacterial nanoparticles have been engineered using traditional antibiotics that are either incorporated within the particle scaffold or attached to the exterior of the particle. In many cases, such particles have exhibited greater efficacy than their constituent antibiotics alone. For example, Gu et al. reported that vancomycin-capped gold nanoparticles exhibited a 64-fold improvement in efficacy over vancomycin alone. 3 Similarly, silver nanoparticles have schoenfisch@unc.edu. Supporting Information Available: Synthesis of FITC-modified silica nanoparticles, AFM analysis of nanoparticle dimensions, scavenging of NO by TSB, and confocal fluorescence microscopy images of PROLI/NO-treated P. aeruginosa cells. This material is available free of charge via the Internet at http://pubs.acs.org. NIH Public AccessAuthor Manuscript ACS Nano. Author manuscript; available in PMC 2013 February 13. shown greater antibacterial activity than silver ion (Ag + ) in solution due to the direct toxicity of the particles and tunable release of Ag + based on nanocomposite size. [4][...

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“…They have the potential for substantial loading, zero premature release of proteins, and highly efficient cellular uptake [10,28,29]. Finally, MSNs are able to aid in effective endosomal escape due to the pH buffering properties naturally found on silica nanomaterials which destabilizes the endosomal membrane (the proton sponge effect) [15,20,30,31].…”

Section: Mesoporous Silica Nanoparticles: Ideal Candidates For Proteimentioning

confidence: 99%

Controlled release and intracellular protein delivery from mesoporous silica nanoparticles

Deodhar

Adams

Trewyn

2016

Biotechnology Journal

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Protein therapeutics are promising candidates for disease treatment due to their high specificity and minimal adverse side effects; however, targeted protein delivery to specific sites has proven challenging. Mesoporous silica nanoparticles (MSN) have demonstrated to be ideal candidates for this application, given their high loading capacity, biocompatibility, and ability to protect host molecules from degradation. These materials exhibit tunable pore sizes, shapes and volumes, and surfaces which can be easily functionalized. This serves to control the movement of molecules in and out of the pores, thus entrapping guest molecules until a specific stimulus triggers release. In this review, we will cover the benefits of using MSN as protein therapeutic carriers, demonstrating that there is great diversity in the ways MSN can be used to service proteins. Methods for controlling the physical dimensions of pores via synthetic conditions, applications of therapeutic protein loaded MSN materials in cancer therapies, delivering protein loaded MSN materials to plant cells using biolistic methods, and common stimuli‐responsive functionalities will be discussed. New and exciting strategies for controlled release and manipulation of proteins are also covered in this review. While research in this area has advanced substantially, we conclude this review with future challenges to be tackled by the scientific community.

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Well-Ordered Mesoporous Silica Nanoparticles as Cell Markers

Cited by 420 publications

References 29 publications

Enhanced Chemotherapy of Cancer Using pH-Sensitive Mesoporous Silica Nanoparticles to Antagonize P-Glycoprotein–Mediated Drug Resistance

Enhanced Chemotherapy of Cancer Using pH-Sensitive Mesoporous Silica Nanoparticles to Antagonize P-Glycoprotein–Mediated Drug Resistance

Bactericidal Efficacy of Nitric Oxide-Releasing Silica Nanoparticles

Controlled release and intracellular protein delivery from mesoporous silica nanoparticles

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