This paper presents the results of 415 d of pilot-scale submerged membrane bioreactor (SMBR) experiments on settled municipal wastewater. Steady-state fouling rates were determined for 10, 5, 4, 3, and 2-d MCRTs, corresponding to median food to microorganism (F/M) ratios of 0.34, 0.55, 0.73, 0.84, and 1.41 gCOD/gVSS . d, respectively. Membrane fouling increased as the F/M was increased. The membrane flux and the mixed liquor suspended solids (MLSS) concentration were constant at 30 L/m 2. h and 8±2 g/L, respectively. The quantities of extracellular polymeric substances (EPS) and soluble microbial products (SMP) were quantified as protein and carbohydrate for each steady-state condition. The total EPS was not a function of MCRT, but the ratio of protein to carbohydrate EPS P/C decreased as the MCRT was decreased. Carbohydrate SMP seemed to be responsible for increased fouling rates at high loading rates with a significant increase occurring from an approximate value of 20 mg/L at all previous conditions (10, 5, 4, to 30 mg/L at the 2-d MCRT (F/M = 1.41 gCOD/gVSS . d). Soluble COD did not correlate with steady-state membrane fouling rates or membrane permeability.