Wet electrospun silk fibroin/gold nanoparticle 3D matrices for wound healing applications

Aktürk, Ömer; Kısmet, Kemal; Yastı, Ahmet Çınar; Kuru, Serdar; Duymuş, Mahmut; Kaya, Feridun; Çaydere, Muzaffer; Hücümenoğlu, Sema; Keskin, Dilek

doi:10.1039/c5ra24225h

Cited by 61 publications

(22 citation statements)

References 89 publications

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“…A cell culture experiment could be the first step for testing the biocompatibility of natural or modified materials 30 , 31 . Isolated corneal epithelial cells, endothelial cells, corneal keratocytes, and limbal stem cells have been used as a testing protocol in ophthalmological applications 12 .…”

Section: Methodsmentioning

confidence: 99%

Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method

Kim

Park

2018

Sci Rep

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Tissue engineering has significantly contributed to the development of optimal treatments for individual injury sites based on their unique functional and histologic properties. Human organs and tissue have three-dimensional (3D) morphologies; for example, the morphology of the eye is a spherical shape. However, most conventional electrospinning equipment is only capable of fabricating a two-dimensional (2D) structured fibrous scaffold and no report is available on a 3D electrospinning method to fabricate a hemispherical scaffold to mimic the native properties of the cornea, including microscopic to macroscopic morphology and transparency. We proposed a novel electrospinning method using a single nonconductive hemispherical device and a metal pin. A designed peg-top shaped collector, a hemispherical nonconductive device with a metal pin in the center and copper wire forming a circle around at the edge was attached to a conventional conductive collector. A 3D hemispherical transparent scaffold with radially aligned nanofibers was successfully fabricated with the designed peg-top collector. In summary, our fabricated 3D electrospun scaffold is expected to be suitable for the treatment of injuries of ocular tissues owing to the hemispherical shape and radially aligned nanofibers which can guide the direction of the main collagen and cellular actin filament in the extracellular matrix.

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Section: Methodsmentioning

confidence: 99%

Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method

Kim

Park

2018

Sci Rep

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“…AuNPs also can be applied for wound healing applications. Akturk et al have observed better wound closure in AuNPs containing wet electrospun silk fibroin compared to control groups (Akturk et al, 2016). Higher neovascularization and granulation tissue formation have also been observed compared to untreated skin control group.…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%

Use of Nanoparticles in Tissue Engineering and Regenerative Medicine

Fathi‐Achachelouei

Knopf‐Marques

Silva

et al. 2019

Front. Bioeng. Biotechnol.

289

146

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Advances in nanoparticle (NP) production and demand for control over nanoscale systems have had significant impact on tissue engineering and regenerative medicine (TERM). NPs with low toxicity, contrasting agent properties, tailorable characteristics, targeted/stimuli-response delivery potential, and precise control over behavior (via external stimuli such as magnetic fields) have made it possible their use for improving engineered tissues and overcoming obstacles in TERM. Functional tissue and organ replacements require a high degree of spatial and temporal control over the biological events and also their real-time monitoring. Presentation and local delivery of bioactive (growth factors, chemokines, inhibitors, cytokines, genes etc.) and contrast agents in a controlled manner are important implements to exert control over and monitor the engineered tissues. This need resulted in utilization of NP based systems in tissue engineering scaffolds for delivery of multiple growth factors, for providing contrast for imaging and also for controlling properties of the scaffolds. Depending on the application, materials, as polymers, metals, ceramics and their different composites can be utilized for production of NPs. In this review, we will cover the use of NP systems in TERM and also provide an outlook for future potential use of such systems.

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“…Different NPs containing silk materials have been developed for tissue engineering. Gold NPs can be incorporated into the silk electrospinning solution to obtain silk nanofibers with well-dispersed gold NPs by traditional (Cohen-karni et al, 2012 ) and wet (Akturk et al, 2016 ) electrospinning techniques. In both cases, the incorporation of gold NPs into electrospun silk materials resulted in improved mechanical properties.…”

Section: Silk-based Bionanocompositesmentioning

confidence: 99%

“…In addition, cell attachment was enhanced by functionalizing the gold NPs with the integrin binding peptides RGD. The resultant materials were tested in vivo for wound closure (Akturk et al, 2016 ). No significant differences were found between silk with or without the gold NPs.…”

Section: Silk-based Bionanocompositesmentioning

confidence: 99%

Silk Polymers and Nanoparticles: A Powerful Combination for the Design of Versatile Biomaterials

et al. 2020

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Silk fibroin (SF) is a natural protein largely used in the textile industry but also in biomedicine, catalysis, and other materials applications. SF is biocompatible, biodegradable, and possesses high tensile strength. Moreover, it is a versatile compound that can be formed into different materials at the macro, micro- and nano-scales, such as nanofibers, nanoparticles, hydrogels, microspheres, and other formats. Silk can be further integrated into emerging and promising additive manufacturing techniques like bioprinting, stereolithography or digital light processing 3D printing. As such, the development of methodologies for the functionalization of silk materials provide added value. Inorganic nanoparticles (INPs) have interesting and unexpected properties differing from bulk materials. These properties include better catalysis efficiency (better surface/volume ratio and consequently decreased quantify of catalyst), antibacterial activity, fluorescence properties, and UV-radiation protection or superparamagnetic behavior depending on the metal used. Given the promising results and performance of INPs, their use in many different procedures has been growing. Therefore, combining the useful properties of silk fibroin materials with those from INPs is increasingly relevant in many applications. Two main methodologies have been used in the literature to form silk-based bionanocomposites: in situ synthesis of INPs in silk materials, or the addition of preformed INPs to silk materials. This work presents an overview of current silk nanocomposites developed by these two main methodologies. An evaluation of overall INP characteristics and their distribution within the material is presented for each approach. Finally, an outlook is provided about the potential applications of these resultant nanocomposite materials.

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Wet electrospun silk fibroin/gold nanoparticle 3D matrices for wound healing applications

Abstract: The effectiveness of a silk fibroin/gold nanoparticle 3D nanofibrous matrix on a rat model of full-thickness dermal wound healing was investigated.

Cited by 61 publications

References 89 publications

Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method

Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method

Use of Nanoparticles in Tissue Engineering and Regenerative Medicine

Silk Polymers and Nanoparticles: A Powerful Combination for the Design of Versatile Biomaterials

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