Wetlands play an important role in the terrestrial ecosystem. However, agricultural activities have resulted in a significant decrease in natural wetlands around the world. In the Tumen River Basin (TRB), a border area between China, the Democratic People’s Republic of Korea (DPRK), and Russia, natural wetlands have been reclaimed and converted into farmland, primarily due to the migration practices of Korean-Chinese. To understand the spatial and temporal patterns of this conversion from wetlands to farmland, Landsat remote sensing images from four time periods were analyzed. Almost 30 years of data were extracted using the object-oriented classification method combined with random forest classification. In addition, statistical analysis was conducted on the conversion from natural wetland to farmland and from farmland to wetland, as well as on the relationship between the driving factors. The results revealed that a loss of 49.2% (12,540.1 ha) of natural wetlands in the Chinese portion of the TRB was due to agricultural encroachment for grain production. At the sub-basin scale, the largest area of natural wetland converted into farmland in the past 30 years was in the Hunchun River Basin (HCH), which accounts for 22.0% (2761.2 ha) of the total. Meanwhile, 6571.4 ha of natural wetlands, mainly in the Gaya River Basin (GYH), have been restored from farmland. These changes are closely related to the migration of the agricultural populations.