Wettability of substrata controls cell–substrate and cell–cell adhesions

Tzoneva, Rumiana; Faucheux, Nathalie; Groth, Thomas

doi:10.1016/j.bbagen.2007.07.008

Cited by 124 publications

(98 citation statements)

References 50 publications

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“…below 20° after contact with serum). This is very different from other types of surfaces, such as self-assembled monolayers (SAMs) presenting very different functional groups (such as methyl, vinyl, amine, carboxyl, hydroxyl [38] ), for which water contact angles span a much larger range (15°-90°). In the case of SAMs, differences in cell attachment [39] as well as differences in myoblast differentiation [2] could be attributed to film chemistry and/ or wettability.…”

Section: Relative Importance Of Film Stiffness Roughness and Wettabimentioning

confidence: 78%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

246

263

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Beside chemical properties and topographical features, mechanical properties of gels have been recently demonstrated to play an important role in various cellular processes, including cell attachment, proliferation, and differentiation. In this work, we used multilayer films made of poly (L-lysine)/Hyaluronan (PLL/HA) of controlled stiffness to investigate the effects of mechanical properties of thin films on skeletal muscle cells (C2C12 cells) differentiation. Prior to differentiation, cells need to adhere and proliferate in growth medium. Stiff films (E 0 > 320 kPa) promoted formation of focal adhesions and organization of the cytoskeleton as well as an enhanced proliferation, whereas soft films were not favorable for cell anchoring, spreading or proliferation. Then C2C12 cells were switched to a low serum containing medium to induce cell differentiation, which was also greatly dependent on film stiffness. Although myogenin and troponin T expressions were only moderately affected by film stiffness, the morphology of the myotubes exhibited striking stiffness-dependent differences. Soft films allowed differentiation only for few days and the myotubes were very short and thick. Cell clumping followed by aggregates detachment could be observed after ~2 to 4 days. On stiffer films, significantly more elongated and thinner myotubes were observed for up to ~ 2 weeks. Myotube striation was also observed but only for the stiffer films. These results demonstrate that film stiffness modulates deeply adhesion, proliferation and differentiation, each of these processes having its own stiffness requirement.

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Section: Relative Importance Of Film Stiffness Roughness and Wettabimentioning

confidence: 78%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

246

263

View full text Add to dashboard Cite

show abstract

“…The accessibility of specific domains was increased through remodeling of this adsorbed proteins. 35 The presence of both immobilized gelatin and adsorbed proteins was believed to improve cell affinity, resulting in better cell spreading and abundant focal adhesions observed on PLLA-gAA-gelatin in Fig. 7.…”

Section: Discussionmentioning

confidence: 99%

“…However, the stronger protein binding and unfolding of protein due to the dehydration mechanism on hydrophobic surface are probably casing conformation changes in the protein molecules leading to a decreased accessibility of specific epitopes on PLLA. 35 Consequently, only a small number of integrin was able to activated through binding with cell-adhesion domains, leading to less focal adhesions observed on PLLA than that on two modified PLLA substrates ͑Fig. 7͒.…”

Section: Discussionmentioning

confidence: 99%

“…Contact angle measurement showed in Table II indicated that immobilization of gelatin and chitosan increased surface wettability, which has been shown to be one of important surface properties affecting cell-substrate adhesion. 35 The modified PLLA substrate was further evaluated by in vitro study of endothelial cell interactions. Results suggested that immobilizing gelatin and chitosan on PLLA improves cell adhesion ͑Fig.…”

Section: Discussionmentioning

confidence: 99%

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Surface modification of poly(l-lactic acid) with biomolecules to promote endothelialization

Xia¹,

Boey²,

Venkatraman³

2010

Biointerphases

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“…Wettability and SFE are key parameters determining the adsorption of biomolecules onto surfaces and therefore controlling the adhesion of cells and bacteria 58,59 . Moreover, changes in the wettability of the samples can be used to monitor the process of functionalization ( Table 1).…”

Section: Physicochemical Characterization Of the Surfacesmentioning

confidence: 99%

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

et al. 2015

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Dental implant failure can be associated to infections which develop into periimplantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMP) have been studied. To covalently immobilize these molecules onto metallic substrates several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1-11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro.Smooth titanium samples were coated with hLf1-11 peptide under three different conditions: silanization with APTES, and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the non-fouling nature of polymer PDMA segment.3

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Wettability of substrata controls cell–substrate and cell–cell adhesions

Cited by 124 publications

References 50 publications

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Surface modification of poly(l-lactic acid) with biomolecules to promote endothelialization

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

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