WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat

Paudel, Bimal; Zhuang, Yongbin; Galla, Aravind; Dahal, Subha; Qiu, Yefeng; Ma, Anjun; Raihan, Tajbir; Yen, Yang

doi:10.1038/s41598-020-64777-9

Cited by 18 publications

(15 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, HRC was expressed in many genotypes with varying resistance levels, albeit to a much weaker extent in lines without the Fhb1 resistance allele. This expression pattern is inconsistent with the proposed susceptibility factor at HRC [9,87].…”

Section: Downstream Activation Of Plant Defense Mechanismcontrasting

confidence: 81%

“…However, the two studies disagreed on the mode of action being either the result of a recessive loss-of-function mutation [9] or a functionally novel allele actively conferring resistance [8]. Moreover, recently, Paudel et al [87] claimed that HRC acts as suppressor of WFhb1-1, which they suggested as the functional component of Fhb1. WFhb1-1 is located outside the QTL interval, but the deletion in HRC inactivates its suppression and results in the 'resistant HRC allele' [87].…”

Section: Host Defense Responses To Limit Fusarium Spreadmentioning

confidence: 99%

“…Moreover, recently, Paudel et al [87] claimed that HRC acts as suppressor of WFhb1-1, which they suggested as the functional component of Fhb1. WFhb1-1 is located outside the QTL interval, but the deletion in HRC inactivates its suppression and results in the 'resistant HRC allele' [87].…”

Section: Host Defense Responses To Limit Fusarium Spreadmentioning

confidence: 99%

See 2 more Smart Citations

Fusarium Head Blight Resistance in European Winter Wheat: Insights From Genome-Wide Transcriptome Analysis

Buerstmayr

Wagner

Nosenko

et al. 2021

Preprint

View full text Add to dashboard Cite

Background Fusarium head blight (FHB) is a devastating disease of wheat worldwide. Resistance to FHB is quantitatively controlled by the combined effects of many small to medium effect QTL. Flowering traits, especially the extent of extruded anthers, are strongly associated with FHB resistance. Results To characterize the genetic basis of FHB resistance, we generated and analyzed phenotypic and gene expression data on the response to Fusarium graminearum (Fg) infection in 96 European winter wheat genotypes, including several lines containing introgressions from the highly resistant Asian cultivar Sumai3. The 96 lines represented a broad range in FHB resistance and were assigned to sub-groups based on their phenotypic FHB severity score. Comparative analyses were conducted to connect sub-group-specific expression profiles in response to Fg infection with FHB resistance level. Collectively, over 12300 wheat genes were Fusarium responsive. The core set of genes induced in response to Fg was common across different resistance groups, indicating that the activation of basal defense response mechanisms was largely independent of the resistance level of the wheat line. Fg-induced genes tended to have higher expression levels in more susceptible genotypes. Compared to the more susceptible non-Sumai3 lines, the Sumai3-derivatives demonstrated higher constitutive expression of genes associated with cell wall and plant-type secondary cell wall biogenesis and higher constitutive and Fg-induced expression of genes involved in terpene metabolism. Gene expression analysis of the FHB QTL Qfhs.ifa-5A identified a constitutively expressed gene encoding a stress response NST1-like protein (TraesCS5A01G211300LC) as a candidate gene for FHB resistance. NST1 genes are key regulators of secondary cell wall biosynthesis in anther endothecium cells. Whether the stress response NST1-like gene affects anther extrusion, thereby affecting FHB resistance, needs further investigation. Conclusion Induced and preexisting cell wall components and terpene metabolites contribute to resistance and limit fungal colonization early on. In contrast, excessive gene expression directs plant defense response towards programmed cell death which favors necrotrophic growth of the Fg pathogen and could thus lead to increased fungal colonization.

show abstract

Section: Downstream Activation Of Plant Defense Mechanismcontrasting

confidence: 81%

Section: Host Defense Responses To Limit Fusarium Spreadmentioning

confidence: 99%

See 1 more Smart Citation

Fusarium Head Blight Resistance in European Winter Wheat: Insights From Genome-Wide Transcriptome Analysis

Buerstmayr

Wagner

Nosenko

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…QTL regions were identified in RefSeqv1.0 based BLASTn analysis of markers Gwm133 and Gwm644 on chromosome arm 6BS for Fhb2 , ( Cuthbert et al, 2007 ), and Gwm304 and Gwm415 on chromosome arm 5AS for Fhb5 ( Xue et al, 2011 ). The Fhb1 genomic region was identified using sequences of candidate genes and gene containing contigs [ Fhb1-1 , GenBank accession KU304333.1 ( Paudel et al, 2020 ); PFT and PFT containing contig, GenBank accessions AY587018.1 and KX907434.1 ( Rawat et al, 2016 ); and TaHRC , GenBank accession MK450312.1 ( Su et al, 2019 )].…”

Section: Methodsmentioning

confidence: 99%

Histology and RNA Sequencing Provide Insights Into Fusarium Head Blight Resistance in AAC Tenacious

Nilsen

Walkowiak²,

Kumar

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Fusarium head blight (FHB) is a serious fungal disease affecting wheat and other cereals worldwide. This fungus causes severe yield and quality losses from a reduction in grain quality and contamination of grain with mycotoxins. Intensive breeding efforts led to the release of AAC Tenacious, which was the first spring wheat cultivar registered in Canada with a resistant (R) rating to FHB. To elucidate the physiological mechanisms of resistance, we performed histological and transcriptomic analyses of AAC Tenacious and a susceptible control Roblin after inoculation with Fusarium graminearum (Fg). The spikelet and rachis of infected wheat spikes were hand sectioned and monitored by confocal and fluorescent microscopy. Visible hyphae were observed within the inoculated spikelets for AAC Tenacious; however, the infection was largely restricted to the point of inoculation (POI), whereas the adjacent florets in Roblin were heavily infected. Significant cell wall thickening within the rachis node below the POI was evident in AAC Tenacious compared to Roblin in response to Fg inoculation. Rachis node and rachilla tissues from the POI and the rachis node below the POI were collected at 5 days post inoculation for RNAseq. Significant changes in gene expression were detected in both cultivars in response to infection. The rachis node below the POI in AAC Tenacious had fewer differentially expressed genes (DEGs) when compared to the uninoculated control, likely due to its increased disease resistance. Analysis of DEGs in Roblin and AAC Tenacious revealed the activation of genes and pathways in response to infection, including those putatively involved in cell wall modification and defense response.

show abstract

“…Although many determinants were already described as associated with wheat FHB resistance, the specific molecular mechanisms responsible for FHB resistance remain poorly understood (Wang et al, 2019), as exemplified by the QTL Fhb1 characterized as the most stable and efficient locus for wheat resistance to FHB (Bai et al, 1999;Ollier et al, 2020). While recent studies have shown that different mutations inducing the TaHRC gene loss of function explain part of this Fhb1-mediated resistance (Li et al, 2019;Su et al, 2019), further works have either suggested a role for the WFhb1-1 gene through its potential antifungal activity (Paudel et al, 2020) or for the TaLAC4 gene whose mutation leads to an increase of FHB susceptibility (Soni et al, 2020). In addition to the current sources of FHB resistance, several works introduced alternative forms of resistance via the identification of wheat genes involved in the success of the disease (reviewed in Fabre et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Proteomics-Based Data Integration of Wheat Cultivars Facing Fusarium graminearum Strains Revealed a Core-Responsive Pattern Controlling Fusarium Head Blight

et al. 2021

View full text Add to dashboard Cite

Fusarium head blight (FHB), mainly occurring upon Fusarium graminearum infection in a wide variety of small-grain cereals, is supposed to be controlled by a range of processes diverted by the fungal pathogen, the so-called susceptibility factors. As a mean to provide relevant information about the molecular events involved in FHB susceptibility in bread wheat, we studied an extensive proteome of more than 7,900 identified wheat proteins in three cultivars of contrasting susceptibilities during their interaction with three F. graminearum strains of different aggressiveness. No cultivar-specific proteins discriminated the three wheat genotypes, demonstrating the establishment of a core proteome regardless of unequivocal FHB susceptibility differences. Quantitative protein analysis revealed that most of the FHB-induced molecular adjustments were shared by wheat cultivars and occurred independently of the F. graminearum strain aggressiveness. Although subtle abundance changes evidenced genotype-dependent responses to FHB, cultivar distinction was found to be mainly due to basal abundance differences, especially regarding the chloroplast functions. Integrating these data with previous proteome mapping of the three F. graminearum strains facing the three same wheat cultivars, we demonstrated strong correlations between the wheat protein abundance changes and the adjustments of fungal proteins supposed to interfere with host molecular functions. Together, these results provide a resourceful dataset that expands our understanding of the specific molecular events taking place during the wheat–F. graminearum interaction.

show abstract

WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat

Cited by 18 publications

References 50 publications

Fusarium Head Blight Resistance in European Winter Wheat: Insights From Genome-Wide Transcriptome Analysis

Fusarium Head Blight Resistance in European Winter Wheat: Insights From Genome-Wide Transcriptome Analysis

Histology and RNA Sequencing Provide Insights Into Fusarium Head Blight Resistance in AAC Tenacious

Proteomics-Based Data Integration of Wheat Cultivars Facing Fusarium graminearum Strains Revealed a Core-Responsive Pattern Controlling Fusarium Head Blight

Contact Info

Product

Resources

About