What about Platelet Function in Platelet Concentrates?

Prudent, Michel

doi:10.1055/a-1210-3229

Cited by 10 publications

(18 citation statements)

References 72 publications

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“…In our study, platelet inactivation on day 1 was likely due to "platelet storage lesion", a phenomenon resulting in the platelet's disc-to-sphere transformation and known to be associated with reduced responsiveness to adenosine diphosphate for platelet aggregation [22]. We believe our observation was due to hypoxia, an effect of the sample resting on the bench.…”

Section: Discussionmentioning

confidence: 63%

How Circulating Cancer Cells Disguise: the Role of Platelets

Schott¹,

Pizon²,

Schill³

et al. 2021

Preprint

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Solid tumors are notorious for their ability to form lethal metastases, sometimes several decades following initial cancer diagnosis. Development of distant metastases is a result of the primary tumor shedding cells that travel via lymphatics and the blood to distant sites where they can form metastases. Platelets are known to specifically enhance tumor cells’ survival in the bloodstream by as yet poorly understood mechanisms. To study the interplay of platelets with circulating tumor cells, we implemented our published approach to label both circulating epithelial tumor cells and platelets. Blood samples were collected avoiding fixation from patients with non-metastatic cancer diagnoses and processed at 4 time points following blood collection. Circulating epithelial tumor cells were undetectable directly after blood collection but became visible after overnight storage at room temperature presumably due to release of platelets from the tumor cells. Our results suggest that platelets play a key role in masking circulating tumor cells. Masking may explain the difficulties in detection of these cells and prevention of their elimination by the immune system. Our unmasking approach detects sufficient numbers of circulating tumor cells to monitor the effect on blood tumor cells of different therapeutic measures, thus contributing to improved systemic therapies for cancer.

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Section: Discussionmentioning

confidence: 63%

How Circulating Cancer Cells Disguise: the Role of Platelets

Schott¹,

Pizon²,

Schill³

et al. 2021

Preprint

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“…The comparability of pooled and apheresis platelet concentrates is a much-debated topic. 6 To avoid an influence of donor variability as well as of storage, 11 Note: Total blood volume was calculated after the Nadler formula. The pH analysis was performed at the end of storage from the manual transported platelet concentrate (PC).…”

Section: Resultsmentioning

confidence: 99%

“…The comparability of pooled and apheresis platelet concentrates is a much‐debated topic. 6 To avoid an influence of donor variability as well as of storage, 11 we used apheresis platelet concentrates on the day of donation. The strength of this study was that each concentrate presented its own control as each of it was split into three equal parts.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, this three‐arm study was designed to comparatively evaluate the impact of three different transport modes—PTS, conveyer system, and manual transport—on platelet quality and thus focused on functional tests of apheresis platelet concentrates by accelerometry, aggregometry, thrombelastography, and flow cytometry 11 , 12 while taking the shock load into account.…”

Section: Introductionmentioning

confidence: 99%

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Triple apheresis platelet concentrate quality after pneumatic tube system, conveyor box, and courier transport: An observational study

Reichert

Wallner

Burkhardt

et al. 2022

Health Science Reports

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Background and Aims Platelets are prone to activation from handling; they are therefore transported as gently as possible, most commonly by courier. Speedier methods like pneumatic tube system (PTS) transport could improve patient care but may subject platelets to mechanical stress. To test the impact of mechanical stress caused by transport, we compared a PTS with a conveyor box and courier transport on apheresis platelet function. Methods Fourteen apheresis platelet concentrate triple donations were analyzed by light transmission aggregometry (LTA), rotational thrombelastometry (ROTEM), and flow cytometry before and after indoor transport over 800 m by PTS, conveyor, and courier, respectively, while recording shocks and vibrations with a high‐frequency acceleration data logger. Shock index scores were calculated as shock intensity (g‐force) times frequency. Results The shock index was 81 for courier, 6279 for conveyor, and 9075 for PTS. Flow cytometry revealed no significant difference in platelet surface expression of CD62p before (16%) and after transport via courier (15%), conveyor (14%), or PTS (16%). LTA with adenosine phosphate and thrombin receptor‐activating peptide‐6 resulted in comparable platelet aggregation for courier, conveyor, and PTS. ROTEM assays showed no relevant differences in coagulation time, clot formation time, and maximum clot firmness between transport modes. Conclusion Though the mechanical challenge was smallest with courier transport, there were no significant differences in platelet activation or aggregation between the three transport modes. These data contradict restrictions on the use of PTSs for platelet concentrate transport.

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“…Further studies should be conducted to check if those biotinylation processes could be combined with different processes or storage conditions, such as Intercept treatment after the biotinylation, refrigeration, or cryopreserve BioPLTs storage 31 . All these parameters could be implemented in clinical trials to evaluate the in vivo function of transfused platelets and to investigate the impact of PC processing and storage conditions on the efficacy of transfusion 14 …”

Section: Discussionmentioning

confidence: 99%

Two novel platelet biotinylation methods and their impact on stored platelet concentrates in a blood bank environment

et al. 2022

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Background: Storage of platelet concentrates (PCs) has an impact on platelet quality and possibly affects their functions after transfusion. The influence of processing and storage conditions of PCs on their in vivo function upon transfusion is unknown. One option for investigating this question is to implement an ex vivo labeling of human platelets, to analyze them after transfusion into heathy volunteers and/or patients. In this study, we developed two labeling methods employing biotin. Methods: Two methods of biotinylation were compared to a control (standard PC). The "Bio-Wash" process used washing steps to label all platelets within the PC; for the other method, "Bio-Direct," one fifth of the PC were directly labeled without washing steps. The control and the two biotinylated PCs were analyzed over 7 days of storage. Labeling efficiency, platelet counts, phenotypes, and functions, along with time and costs, were evaluated to select the best process.Results: Both methods achieved a stable labeling through the storage, with similar platelet counts and metabolism in comparison to control PCs. Bio-Wash showed higher activation phenotype and lower aggregation response in comparison to the Bio-Direct method. The Bio-Direct was performed within 1.5 h versus 3 h for the Bio-Wash. However, the Bio-Direct required 12 mg of biotin instead of 8 mg for the other process. Conclusion: We set up two methods of biotinylation that can be easily implemented in a blood bank environment. The Bio-Direct process was preferred to the Bio-Wash because of its similarity, from a functional and phenotypic point of view, with standard PCs.

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What about Platelet Function in Platelet Concentrates?

Cited by 10 publications

References 72 publications

How Circulating Cancer Cells Disguise: the Role of Platelets

How Circulating Cancer Cells Disguise: the Role of Platelets

Triple apheresis platelet concentrate quality after pneumatic tube system, conveyor box, and courier transport: An observational study

Two novel platelet biotinylation methods and their impact on stored platelet concentrates in a blood bank environment

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