What can we learn from ambient ionization techniques?

Chen, Huanwen; Gamez, Gerardo; Zenobi, Renato

doi:10.1016/j.jasms.2009.07.025

Cited by 228 publications

(186 citation statements)

References 137 publications

(229 reference statements)

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“…The DART technique has been used for monitoring tea fermentation and manufacturing (Fraser et al 2013), for the determination of anisatin in teas (Shen et al 2012), for quality assessment of Basha herbal tea (Deng & Yang 2013). This is a relatively new ionisation technique of mass spectrometry, however its principles and advantages are well described in the literature (Chen et al 2009;Hajslová et al 2011;Huang et al 2011).…”

Section: Food Microbiology and Safetymentioning

confidence: 99%

Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods

Žiarovská¹,

Rajchl²,

Lojka³

et al. 2016

Czech J. Food Sci.

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AbstractŽiarovská J., Rajchl A., Fernández E., Prchalová J., Milella L. (2016): Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods. Czech J. Food Sci., 34: 495-502.The identification of yacon, a medicinal plant, in tea mixtures by rapid Polymerase Chain Reaction (PCR) and the Direct Analysis in Real Time coupled with Time-of-Flight Mass Spectrometry (DART/TOF-MS) method were evaluated. Three tea products and a pure yacon tea were analysed using the molecular method PCR, concretely the intraspecific variation of the internal transcribed spacer (ITS) regions of rDNA and the DART method coupled with TOF-MS. The results show the reliability of PCR and restriction cleavage of the ITS as a combined approach to confirm the presence of yacon in herbal tea mixtures. Three fragments of approximately 700, 408, and 235 bp in length are present when yacon is a part of the herbal tea mixture. The Principal Component Analysis (PCA) based on the fingerprints of the complete Total Ion Current (TIC) mass spectra shows sufficient separation of herbal teas with and without yacon leaves. The reported methods are technically rapid and can be used as an effective tool for the purposes of yacon identification or authentication.

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Section: Food Microbiology and Safetymentioning

confidence: 99%

Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods

Žiarovská¹,

Rajchl²,

Lojka³

et al. 2016

Czech J. Food Sci.

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“…[11][12][13][14] Most ambient MS methods were primarily utilized for analysing metabolites and lipids. [15][16][17][18] Protein analysis from dried biological samples such as blood, cell cultures, and tissues was demonstrated by electrospray assisted laser desorption ionization (ELDI) MS. 19 In extracts and crude samples, desorption electrospray ionization (DESI) provided peptide and protein analysis including top down sequencing.…”

Section: 10mentioning

confidence: 99%

Comparative local analysis of metabolites, lipids and proteins in intact fish tissues by LAESI mass spectrometry

Shrestha

Javonillo

Burns

et al. 2013

Analyst

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Direct mass spectrometric analysis of animal tissues is an emerging field enabled by recent developments in ambient ion sources. Label-free in situ analysis of metabolites, lipids, and peptides/proteins from intact tissues in whole fish specimens of different gender and age were performed by laser ablation electrospray ionization (LAESI) mass spectrometry (MS). Hypertrophied glandular tissue (gill gland) of adult male Aphyocharax anisitsi (bloodfin tetra) was compared with gill tissues in females of the same species. Comparison of a large number of sample-specific ions was aided by a multivariate statistical method based on orthogonal projections to latent structures discriminant analysis. More than 200 different ions were detected in the mass spectra corresponding to primary metabolites, hormones, lipids and peptides/proteins. The gill tissues of the sexually mature males exhibited multiply charged ions in the 6+ to 10+ charge states corresponding to a protein with a molecular weight of 11 380 Da. This protein was present only in the mature male gill glands but absent in the corresponding area of the female and immature male specimens. An additional nine proteins were detected by LAESI-MS in both the male and female gill tissues. IntroductionMolecular analysis of proteins, lipids, and metabolites in sh by mass spectrometry (MS) is essential in various aspects of their systems biology, including the understanding of their developmental physiological changes.1,2 Various MS methods are involved in detecting environmental pressures from pharmaceuticals, e.g., antibiotics 3 and contraceptives, 4 to agrochemicals. 5Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) MS have been established as efficient tools to perform proteomic analysis from sh tissue extracts. 6-8Metabolomics studies of sh tissues frequently utilize extraction protocols followed by gas chromatography (GC) MS or high performance liquid chromatography (HPLC) MS. 9,10Direct MS of tissues by recently developed atmospheric pressure ionization methods offers rapid, label-free analysis with minimal sample preparation.11-14 Most ambient MS methods were primarily utilized for analysing metabolites and lipids. [15][16][17][18] Protein analysis from dried biological samples such as blood, cell cultures, and tissues was demonstrated by electrospray assisted laser desorption ionization (ELDI) MS.19 In extracts and crude samples, desorption electrospray ionization (DESI) provided peptide and protein analysis including top down sequencing.20-22 Sensitive analysis of proteins was performed by extractive electrospray ionization mass spectrometry (EESI) by infusing untreated biological samples into the electrospray plume. 23Laser ablation electrospray ionization (LAESI) MS is an ambient ionization technique that employs mid-infrared (mid-IR) laser pulses to locally ablate the tissue or cell sample and electrospray for the ionization of the ejected material.24 The strong absorption maximum of water at 2.94 mm wavelength enables t...

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“…For DART and other atmospheric pressure chemical ionization (APCI) methods, a glow or corona discharge is deployed. Although abundant ion formation occurs when analytes are exposed to discharges, the overall mechanism of ionization remains poorly defined [5][6][7][8][9][10][11][12][13], and the need to understand it has been duly recognized [14,15]. It is agreed that a complex cascade of gas-phase interactions take place before the target molecules are eventually charged [15,16].…”

Section: Introductionmentioning

confidence: 99%

Regulated In Situ Generation of Molecular Ions or Protonated Molecules under Atmospheric-Pressure Helium-Plasma-Ionization Mass Spectrometric Conditions

Gangam

Pavlov

Attygalle

2015

J. Am. Soc. Mass Spectrom.

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Abstract. In an enclosed atmospheric-pressure helium-plasma ionization (HePI) source engulfed with dehumidified ambient gases, molecular cations are generated from compounds such as toluene, bromobenzene, and iodobenzene. Evidently, the ionization is effected by a direct Penning mechanism attributable to interactions of the gas-phase analyte with metastable helium atoms. It is widely known that secondary ions generated from ambient gases also play an important role in the overall ionization process. For example, when the ambient gases bear even traces of moisture, the analytes are ionized by proton transfer reactions with gaseous H 3 O + . In this study, we demonstrate how a controlled variation of experimental conditions can manipulate the abundance of molecular ions and protonated molecules in a HePI source.

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What can we learn from ambient ionization techniques?

Cited by 228 publications

References 137 publications

Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods

Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods

Comparative local analysis of metabolites, lipids and proteins in intact fish tissues by LAESI mass spectrometry

Regulated In Situ Generation of Molecular Ions or Protonated Molecules under Atmospheric-Pressure Helium-Plasma-Ionization Mass Spectrometric Conditions

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