This article considers usage of blue-green algae as biomaterials for creation of motor biofuels. Proliferation of blue-green algae leads to water rotting, destruction of aquatic ecosystems and destruction of rivers and lakes that is why clearing of water bodies from blue-green algae is an urgent task. The object of the study is effect of blended biodiesel fuels from blue-green algae on the environmental and energy performances for the diesel engine. The purpose of the work is experimental study of changes of power and ecological characteristics of automobile diesel engines using petroleum diesel and their mixtures with biofuels derived from blue-green algae. Methods of research are experimental, laboratory ones. Laboratory researches were carried out on an experimental installation based on the serial diesel engine D21A1. As a result of performed experimental researches dependences of changing of the effective engine power on the use of diesel fuel and a mixture of diesel fuel with the received bioactive supplements based on methyl esters of the lipid fraction of blue-green algae Chroococcfles in the amount of 5, 10 and 20 % were established. It has been experimentally established that the effective power of an engine using a mixture of diesel fuel with the derived bioactive compounds based on methyl esters of the lipid fraction of blue-green algae Chroococcfles in the amount of 5, 10 and 20 % will decrease by an average of 0,9, 1,8 and 3,5 %. It has been experimentally determined that the content of carbon monoxide in the use of a mixture of diesel fuel with the derived bioactive compounds based on methyl esters of the lipid fraction of blue-green algae Chroococcfles in the amount of 5, 10 and 20 % will decrease by an average of 6,5, 13,9 and 28,7 %. The obtained results allow to optimize the choice of fuels for power systems of internal combustion engines and to reduce emissions of harmful substances in exhaust gases of automobile diesel engines.