What does calorimetry and thermodynamics of living cells tell us?

Maskow, Thomas; Paufler, Sven

doi:10.1016/j.ymeth.2014.10.035

Cited by 50 publications

(32 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported by Maskow and Paufler [25], cell thermogenesis is mainly due to glucose catabolism, the main source of cell energy [26,27]. Results demonstrated that Cd induced an alteration of glucose utilization by fibroblasts, whereas Ni and Al appeared to have no effect.…”

Section: Thermal Analysissupporting

confidence: 64%

Effect of Ni(II), Cd(II) and Al(III) on human fibroblast bioenergetics, a preliminary comparative study

Belliardo

Gallice

Chaspoul

et al. 2016

J Therm Anal Calorim

View full text Add to dashboard Cite

International audienceExposure to Ni, Cd or Al leads to different health issues depending on the dose and the exposure frequency. Their mechanism of action is poorly known, but as metals, they may have some points in common. The aim of this work was to compare the impact on cell bioenergetic of these metals using a common cell model: a normal human dermal fibroblast (NHDF) in primary culture. To study cell bioenergetics which “concerns energy conservation and conversion processes in a living cell” as defined by Demirel and Sandler, two technics are combined: oximetry and microcalorimetry. The heat flow measured by microcalorimetry reflects cell metabolism and more generally glucose catabolism (the only nutriment brought to the NHDF). Cell respiration is measured by oximetry and shows the impact on the mitochondria, the energy factory of the cell. Without incubation, Cd inhibits thermogenesis and cell respiration, Ni has no effect, and Al inhibits cell respiration but not thermogenesis. After 24 h of contact at 40 A mu M, NHDF died with Cd but seemed over-activated with Al and Ni (thermogenesis and cell respiration increased)

show abstract

Section: Thermal Analysissupporting

confidence: 64%

Effect of Ni(II), Cd(II) and Al(III) on human fibroblast bioenergetics, a preliminary comparative study

Belliardo

Gallice

Chaspoul

et al. 2016

J Therm Anal Calorim

View full text Add to dashboard Cite

show abstract

“…The unique weakness of the method is the need to ventilate ampoules daily. Otherwise, oxygen depletion and metabolic waste accumulation in the form of carbon dioxide are the consequences . This final problem can be overcome in future research through the development of an ampoule sealing that is permeable to oxygen and carbon dioxide and impermeable to water .…”

Section: Discussionmentioning

confidence: 99%

“…–460 kJ mol‐O 2 −1 . It is known, that mixed fermentative‐respiratory metabolisms and biological combustion processes, which deviate from the oxycaloric equivalent, can lead to considerable miscalculations of the metabolic heat from respirometric measurements . Temperature increases in SSF, especially in large‐scale processes, could be more accurately predicted and prevented, if reliable, directly measured metabolic heat data were available.…”

Section: Introductionmentioning

confidence: 99%

A simple method for the measurement of metabolic heat production rates during solid‐state fermentations using β‐carotene production with Blakeslea trispora as a model system

Altwasser

Pätz

Lemke³

et al. 2017

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

Solid-state fermentation (SSF) technology has been rapidly developed for the past 10 years as a production platform for secondary metabolites, biofuels, food, and pharmaceuticals. Yet, the main drawback of SSF is the local temperature rise of up to 20 K, which potentially reduces the strain activity and inactivates heat sensible products. Due to the low heat capacity and thermal conductivity of mixtures of air with plant material, in comparison to aqueous suspensions in submerged fermentations, heat from metabolic processes is less efficiently dissipated. The exact knowledge of the metabolic heat generation during SSF processes is crucial to guide strategies against overheating. In this work, a simple method using a cost-efficient multichannel instrument is proposed, which allows the determination of heat generation during SSF processes. This method was successfully tested and validated with Blakeslea trispora producing β-carotene during growth on barley. Additionally, the consequences of the generated metabolic heat during SSF on temperature rise and water evaporation were discussed. Finally, changes in growth and product concentration could also be detected by the heat signal, implying the potential as a timesaving screening method.

show abstract

“…In the literature, it has been reported that mammalian cells produce very little heat in contrast to microbial cells [30,40]. More specifically, the specific heat flow rate of the CHO 320 cell line was evaluated at its peak at 20 pW cell −1 [23].…”

Section: Culture Predictionsmentioning

confidence: 99%

The Application of Dielectric Spectroscopy and Biocalorimetry for the Monitoring of Biomass in Immobilized Mammalian Cell Cultures

2015

View full text Add to dashboard Cite

Abstract:The purpose of this study was to introduce dielectric spectroscopy and biocalorimetry as monitoring methods to follow immobilised Chinese Hamster Ovary (CHO) cell culture development. The theory behind both monitoring techniques is explained and perfusion cultures are performed in a Reaction Calorimeter (eRC1 from Mettler Toledo) as an application example. The findings of this work show that dielectric spectroscopy gives highly reliable information upon the viable cell density throughout the entire culture. On the other hand, the RC1 could only provide accurate data from day 5, when the cell density exceeded 4 × 10 , a viable cell density commonly achieved in fed-batch and the early stages of a perfusion culture. This work suggests that biocalorimetry should be possible to implement at industrial scale to monitor CHO cell cultures.

show abstract

What does calorimetry and thermodynamics of living cells tell us?

Cited by 50 publications

References 78 publications

Effect of Ni(II), Cd(II) and Al(III) on human fibroblast bioenergetics, a preliminary comparative study

Effect of Ni(II), Cd(II) and Al(III) on human fibroblast bioenergetics, a preliminary comparative study

A simple method for the measurement of metabolic heat production rates during solid‐state fermentations using β‐carotene production with Blakeslea trispora as a model system

The Application of Dielectric Spectroscopy and Biocalorimetry for the Monitoring of Biomass in Immobilized Mammalian Cell Cultures

Contact Info

Product

Resources

About