This meta-analysis uses 44 existing studies conducted in single-nation contexts (k = 44), constituting an aggregated sample of 20,886 observations (N = 20,886), to assess the potential moderating role of national culture on organisational ambidexterity-performance relationships. The results indicate an overall positive ambidexterity-performance link, which is stronger in countries with low levels of institutional collectivism, high levels of in-group collectivism, low levels of future orientation, low levels of performance orientation, and low levels of uncertainty avoidance. Additionally, counter to expectation, results suggest that the ambidexterity-performance relationship is stronger in countries with high levels of power distance.