What Factors Affect the Separation of Substances Using Thin-Layer Chromatography? An Undergraduate Experiment

Nash, John J.; Meyer, Jeanne; Everson, Barbara

doi:10.1021/ed078p364

Cited by 11 publications

(13 citation statements)

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“…[61,62] Because discovery-basede xperiments and puzzles oblige students to focus and think about their experimental results, the cookbook approachisd iscouraged. [61,62] Because discovery-basede xperiments and puzzles oblige students to focus and think about their experimental results, the cookbook approachisd iscouraged.…”

Section: Organicchemistrymentioning

confidence: 99%

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Green Chemistry in Higher Education: State of the Art, Challenges, and Future Trends

et al. 2018

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Nowadays, there is increasing interest in global sustainability, and thus, university students would like to know how human actions affect the health status of our planet. This is mainly due to their basic knowledge of problems such as global warming and greenhouse gases. Students would like to gain knowledge on how to safeguard the earth for future generations. This must involve changes in education programs at interested institutions and universities. To ensure that future generations of chemists are equipped with proper knowledge, significant efforts are needed. Thus, this article aims to present the history of green chemistry, its milestones, and ideas on how to teach this subject. A discussion of awareness in the field of green chemistry and of existing teaching materials is presented. In addition, green chemistry metrics, which should be known and used by professors and students, are described. Teaching methods for green chemistry are also given, with special attention paid to organic and analytical chemistry education.

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Section: Organicchemistrymentioning

confidence: 99%

“…Less populard iscovery-based experiments try to encourage students to discover the basic principles that underlie purification techniques. [61,62] Because discovery-basede xperiments and puzzles oblige students to focus and think about their experimental results, the cookbook approachisd iscouraged.…”

Section: Organicchemistrymentioning

confidence: 99%

Green Chemistry in Higher Education: State of the Art, Challenges, and Future Trends

et al. 2018

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“…Correct technique helps to troubleshoot chromatographic problems, such as whether the student followed the instructions regarding preparing the developing solvent. It is also easier to teach chromatography with the concept of weak and strong solvents if the TLC is run well, and the evaluation of different TLC procedures could be made into a lab experiment itself …”

Section: Introductionmentioning

confidence: 99%

“…It is also easier to teach chromatography with the concept of weak and strong solvents if the TLC is run well, and the evaluation of different TLC procedures could be made into a lab experiment itself. 12…”

Section: ■ Introductionmentioning

confidence: 99%

Let Us Teach Proper Thin Layer Chromatography Technique!

Silver

2020

J. Chem. Educ.

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Thin layer chromatography (TLC) is a technique widely used in industrial and research chemistry. It is an inexpensive and simple technique, which makes it attractive for undergraduate chemistry lab experiments. Unfortunately, the technique is sometimes incorrectly taught. Incorrect techniques impede the use of TLC in research laboratories. The most common error in teaching TLC technique is solvent vapors inside the chamber not being equilibrated prior to use. The second most common mistake is the use of watch glass covers when beakers are used for TLC developing chambers. Simply replacing the watch glass with aluminum foil, and preparing the developing chamber before the TLC plates, allows time for the developing solvent to saturate the chamber which yields faster, more reproducible results.

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“…The difference in the Rf value might be due to several factors including the amount of material spotted, temperature, solvent system, and chemical nature of absorbent. The condition of temperature, the equilibrium between liquids and vapour in tank, sample impurities are the key to a high range of Rf value [42][43][44]. In addition, the results showed a difference in intensity of colour spots between BEA that extracted from all isolates and BEA standards.…”

Section: Discussionmentioning

confidence: 99%

Identification and Production of Beauvericin by Fusarium subglutinansand F. sacchari from Sugarcane

Mohammed

Al-Ani

2021

Braz. arch. biol. technol.

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Fusarium is producing several important mycotoxins including beauvericin (BEA). Two species of Fusarium viz. F. subglutinans and F. sacchari cause the Pokkahboeng disease of sugarcane. The studies on the occurrence and toxicity of BEA are scarce. Therefore, this study aimed to identify the isolates of Fusarium and detect their ability to produce BEA. The toxicity of BEA was also tested on brine shrimp Artemia salina. Many isolates of Fusarium were isolated from the infected plants of sugarcane in Malaysia. We identified the species of Fusarium according to their morphological characteristics. The capability of Fusarium isolates for producing the BEA was estimated by using a thin layer chromatography. The toxicity bioassay of BEA was conducted on the brine shrimp larvae. The results were identified on F. subglutinans and F. sacchari in 55 isolates of Fusarium. All isolates demonstrated the ability to produce BEA. Interestingly, BEA exhibited variation in toxicity between low toxic to very higher toxicity 100%. F. subglutinans and F. sacchari were able to produce BEA and possibly BEA may be causing toxicity in the host tissue and may be acting as a potential pathogenicity factor. Therefore, we consider BEA as an interesting factor in determining the virulence of fusarium isolate.

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What Factors Affect the Separation of Substances Using Thin-Layer Chromatography? An Undergraduate Experiment

Cited by 11 publications

References 0 publications

Green Chemistry in Higher Education: State of the Art, Challenges, and Future Trends

Green Chemistry in Higher Education: State of the Art, Challenges, and Future Trends

Let Us Teach Proper Thin Layer Chromatography Technique!

Identification and Production of Beauvericin by Fusarium subglutinansand F. sacchari from Sugarcane

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