What happens to the structure of water in cryoprotectant solutions?

Towey, James J.; Soper, A. K.; Dougan, Lorna

doi:10.1039/c3fd00084b

Cited by 53 publications

(57 citation statements)

References 59 publications

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“…These 3 types of clustering tendencies have been previously reported for other aqueous mixtures. [29][30][31] Interestingly, they mimic those found in aqueous micro-emulsions, where they are named Winsor phases I, III and II. 32,33 Winsor I corresponds to direct micelles, Winson III to the bi-continuous phase and Winsor II to the inverse micelles.…”

Section: Snapshotsmentioning

confidence: 93%

Simple and complex disorder in binary mixtures with benzene as a common solvent

Požar

Seguier

Guerche

et al. 2015

Phys. Chem. Chem. Phys.

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Substituting benzene for water in computer simulations of binary mixtures allows one to study the various forms of disorder, without the complications often encountered in aqueous mixtures. In particular, we study the relationship between the local order generated by different types of molecular interactions and the nature of the global disorder, by analyzing the relationship between the concentration fluctuations and the correlation functions and the associated structure factors. Alkane-benzene mixtures are very close to ideal mixtures, despite appreciable short range shape mismatch interactions, acetone-benzene mixtures appear as a good example of regular mixtures, and ethanol-benzene mixtures show large micro-segregation. In the latter case, we can unambiguously demonstrate, unlike in the case of water, the appearance of domain-domain correlations, both in the correlation functions and the structure factor calculated in computer simulations. This finding helps to confirm the existence of a pre-peak in the structure factor associated with the micro-heterogeneity, which was speculated from several of our previous simulations of aqueous-alcohol mixtures. The fact that benzene as a solvent allows us to solve some of the problems that could not be solved with water points towards some of the particularities of water as a solvent, which we discuss herein. The concept of molecular emulsion put forward in our earlier work is useful in formulating these differences between water and benzene through the analogy with direct and inverse micellar aggregates.

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Section: Snapshotsmentioning

confidence: 93%

Simple and complex disorder in binary mixtures with benzene as a common solvent

Požar

Seguier

Guerche

et al. 2015

Phys. Chem. Chem. Phys.

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“…We therefore also investigate the potential of the well‐established cryoprotectants glycerol and trehalose [ 13 ] to enhance DNA origami stability during repeated freezing and thawing by suppressing the formation of crystalline ice. [ 14 ]…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of DNA Origami Nanostructures

Yang

Kielar

Zhu

et al. 2020

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Although DNA origami nanostructures have found their way into numerous fields of fundamental and applied research, they often suffer from rather limited stability when subjected to environments that differ from the employed assembly conditions, that is, suspended in Mg2+‐containing buffer at moderate temperatures. Here, means for efficient cryopreservation of 2D and 3D DNA origami nanostructures and, in particular, the effect of repeated freezing and thawing cycles are investigated. It is found that, while the 2D DNA origami nanostructures maintain their structural integrity over at least 32 freeze–thaw cycles, ice crystal formation makes the DNA origami gradually more sensitive toward harsh sample treatment conditions. Whereas no freeze damage could be detected in 3D DNA origami nanostructures subjected to 32 freeze–thaw cycles, 1000 freeze–thaw cycles result in significant fragmentation. The cryoprotectants glycerol and trehalose are found to efficiently protect the DNA origami nanostructures against freeze damage at concentrations between 0.2 × 10−3 and 200 × 10−3 m and without any negative effects on DNA origami shape. This work thus provides a basis for the long‐term storage of DNA origami nanostructures, which is an important prerequisite for various technological and medical applications.

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“…We examined properties of frozen/thawed dimethylsulfoxide (DMSO), trehalose, the antifreeze protein TrxA-ApAFP752 (AFP) and DMSO+trehalose solutions both experimentally and theoretically using X-ray diffraction (XRD), Raman spectroscopy, Differential Scanning Calorimetry (DSC), molecular dynamics and ab initio modelling [4,11-29]. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states (XRD, Raman spectroscopy, DSC) [27] and put these results in the context of human skin fibroblasts viability after freezing and thawing [28,29].…”

Section: Introductionmentioning

confidence: 99%

Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethyl sulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability

et al. 2017

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In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab-initio modelling (molecular dynamics, DFT), Raman spectroscopy, Differential Scanning Calorimetry and X-Ray Diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells.

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What happens to the structure of water in cryoprotectant solutions?

Cited by 53 publications

References 59 publications

Simple and complex disorder in binary mixtures with benzene as a common solvent

Simple and complex disorder in binary mixtures with benzene as a common solvent

Cryopreservation of DNA Origami Nanostructures

Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethyl sulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability

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