What is in the black box? The discovery of the sigma factor and the subunit structure of E. coli RNA polymerase

Abstract

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Identification of essential genes in Mycobacterium avium subsp. paratuberculosis genome for persistence in dairy calves

“…From the intermediary metabolism category, genes involved in histidine, aromatic amino acids, ATP, protoheme, riboflavin, arginine, and FOLIC acid biosynthesis are also found to be essential for in vitro growth of MAP and H37Rv ( Griffin et al, 2011 ; DeJesus et al, 2017 ; Minato et al, 2019 ). In vitro essential genes from the information pathway category included RNA polymerase sigma factor sigma A (sigA), a transcription initiation factor in bacteria that is a housekeeping regulatory gene active during the exponential phase and facilitates the specific binding of RNA polymerase to gene promoters ( Burgess, 2021 ). SigA was also found to be essential for in vitro growth of H37Rv.…”