2019
DOI: 10.1002/mus.26654
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What is Normal? Neuromuscular junction reinnervation after nerve injury

Abstract: Introduction In this study we present a reproducible technique to assess motor recovery after nerve injury via neuromuscular junction (NMJ) immunostaining and electrodiagnostic testing. Methods Wild‐type mice underwent sciatic nerve transection with repair. Hindlimb muscles were collected for microscopy up to 30 weeks after injury. Immunostaining was used to assess axons (NF200), Schwann cells (S100), and motor endplates (α‐bungarotoxin). Compound motor action potential (CMAP) amplitude was used to assess tibi… Show more

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Cited by 37 publications
(30 citation statements)
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“…After denervation, a population of more rapidly turning over endplates is produced. Endplate turnover remains more rapid after nerve injury until the process of reinnervation is complete and until the more rapidly turning over endplate population has dissipated (Shyng & Salpeter, ; Vannucci et al, ; Xu & Salpeter, ). Endplate turnover does not necessarily directly correlate with reinnervation—that is: turnover can increase even as fewer NMJs remain denervated.…”
Section: Discussionmentioning
confidence: 99%
“…After denervation, a population of more rapidly turning over endplates is produced. Endplate turnover remains more rapid after nerve injury until the process of reinnervation is complete and until the more rapidly turning over endplate population has dissipated (Shyng & Salpeter, ; Vannucci et al, ; Xu & Salpeter, ). Endplate turnover does not necessarily directly correlate with reinnervation—that is: turnover can increase even as fewer NMJs remain denervated.…”
Section: Discussionmentioning
confidence: 99%
“…The functional recovery of motor axons requires successful regeneration not only at the injury site but also at the endplates of the neuromuscular junctions for reinnervation into the target muscle [ 59 ]. The extensor hallucis longus (EHL) muscle is used to observe the reinnervation of the motor axons that regenerate through the sciatic nerves in a neuromuscular junction ( Figure 2 D).…”
Section: In Vivo Axon Injury Modelmentioning
confidence: 99%
“…Self-reinnervation involves peripheral nerve branch transection and immediate 90 repair, resulting in recovery of motor output with long-term, local loss of autogenic muscle proprioception (Cope 91 et al, 1994; Bullinger et al 2011). Self-reinnervation occurs through axonal regrowth and reconnection with 92 denervated tissues over a recovery period of 4-8 weeks (Carr, et al, 2010;Cope, et al, 1994;Gordon & Stein, 93 1982;Vannucci et al, 2019). Reinnervated muscles remain unresponsive to stretch due to synaptic retraction of 94 muscle spindle afferents from spinal cord lamina IX, leading to disconnection from parent motoneuron 95 populations (Alvarez et al, 2011).…”
Section: Investigating the Role Of Proprioception Through Self-reinnementioning
confidence: 99%