What is the benefit of computer-assisted image analysis of liver fibrosis area?

Yoshioka, Kentaro

doi:10.1007/s00535-012-0727-4

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Progressive accumulation of collagen proteins occurs during liver fibrosis progression [ 36 ]. Therefore, the relative fibrotic area can be used to distinguish the different stages of liver fibrosis [ 37 ]. In liver diseases, excessive collagen deposition leads to structural disruption of the normal liver architecture, liver stiffness, portal hypertension, and eventually hepatic failure [ 38 – 40 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of Autoantibodies to Vascular Endothelial Growth Factor Receptor-3 in Bile Duct Ligated Rats and Correlations with a Panel of Traditional Markers of Liver Diseases

et al. 2016

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There is a need for new noninvasive biomarkers (NIBMs) able to assess cholestasis and fibrosis in chronic cholestatic liver diseases (CCLDs). Tumorigenesis can arise from CCLDs. Therefore, autoantibodies to tumor-associated antigens (TAA) may be early produced in response to abnormal self-antigen expression caused by cholestatic injury. Vascular endothelial growth factor receptor-3 (VEGFR-3) has TAA potential since it is involved in cholangiocytes and lymphatic vessels proliferations during CCLDs. This study aims to detect autoantibodies directed at VEGFR-3 during bile duct ligation- (BDL-) induced cholestatic injury in rat sera and investigate whether they could be associated with traditional markers of liver damage, cholestasis, and fibrosis. An ELISA was performed to detect anti-VEGFR-3 autoantibodies in sera of rats with different degree of liver injury and results were correlated with aminotransferases, total bilirubin, and the relative fibrotic area. Mean absorbances of anti-VEGFR-3 autoantibodies were significantly increased from week one to week five after BDL. The highest correlation was observed with total bilirubin (R 2 = 0.8450, P = 3.04e − 12). In conclusion, anti-VEGFR-3 autoantibodies are early produced during BDL-induced cholestatic injury, and they are closely related to cholestasis, suggesting the potential of anti-VEGFR-3 autoantibodies as NIBMs of cholestasis in CCLDs and justifying the need for further investigations in patients with CCLD.

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Section: Discussionmentioning

confidence: 99%

Detection of Autoantibodies to Vascular Endothelial Growth Factor Receptor-3 in Bile Duct Ligated Rats and Correlations with a Panel of Traditional Markers of Liver Diseases

et al. 2016

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“…Therefore, the relative fibrotic area can be used to distinguish the different stages of liver fibrosis (Yoshioka 2013). In liver diseases, excessive collagen deposition leads to structural disruption of the normal liver architecture, liver stiffness, portal hypertension, and eventually hepatic failure (Reynaert, Thompson et al 2002, Mori, Fujii et al 2011, Iredale, Thompson et al 2013.…”

Section: Discussionmentioning

confidence: 99%

Enhancing the biological activity of peptides by chemical or biological functionalization of Turnip mosaic virus-derived nanoparticles

Gamboa¹

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In this thesis, plant-made nanoparticles derived from Turnip mosaic virus (TuMV), a potyvirus, were developed. These Viral Nanoparticles (VNPs) can be exploited as scaffolds for building modified nano-objects and can be used as diagnostic tools, for antibody generation, immunization, or as biomaterials in general. They fall into two biological categories: either enclosing a nucleic acid (virions) or without the nucleic acid (empty virus-like particles, eVLPs). The modifications imposed on VNPs can also be achieved by two main approaches: genetic or chemical conjugation. Each of them has particular pros and cons for nanotechnological deployment. Overall, this is the first case of genetic or chemical conjugation in plant-made potyvirus VNPs. The VNPs were produced either by inoculation of Indian mustard (Brassica juncea) with the infectious TuMV virus, or by agroinfiltration of the tobacco relative Nicotiana benthamiana with the TuMV coat protein cDNA (CP) on a pEAQ vector for high protein expression. As a proof-of-concept, a human thrombin receptor peptide (TR) for the genetic modification, and a vasoactive intestinal peptide and low-molecular-weight compounds (biotin, organometallics) for the chemical modification, were selected. Significant production of TR-CP VLPs and TuMV virions was obtained from plant leaves. VNPs were observed under an electron microscope after immunodecoration with anti-TR antibody or antibiotin antibody, respectively. Both VNPs proved capacity to log-increase the ability of antibody-sensing compared to free TR peptide or free biotin.In this thesis, the possibility of conjugating, genetically and chemically, peptides and low-molecular-weight compounds to Turnip mosaic virus virions or VLPs is demonstrated. This outlines the potential of these VNPs for their use in numerous nanobiotechnological applications.

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What is the benefit of computer-assisted image analysis of liver fibrosis area?

Cited by 2 publications

References 7 publications

Detection of Autoantibodies to Vascular Endothelial Growth Factor Receptor-3 in Bile Duct Ligated Rats and Correlations with a Panel of Traditional Markers of Liver Diseases

Detection of Autoantibodies to Vascular Endothelial Growth Factor Receptor-3 in Bile Duct Ligated Rats and Correlations with a Panel of Traditional Markers of Liver Diseases

Enhancing the biological activity of peptides by chemical or biological functionalization of Turnip mosaic virus-derived nanoparticles

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