What is the relationship between the metabolism of preimplantation embryos and their developmental competence?

Barnett, Deborah K.; Bavister, Barry D.

doi:10.1002/(sici)1098-2795(199601)43:1<105::aid-mrd13>3.0.co;2-4

Cited by 139 publications

(35 citation statements)

References 246 publications

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“…Another study reported that without phosphate, glucose alone had no detrimental effect on early embryonic development (Kim et al 1993). In contrast, Barnett and Bavister (1996) and Biggers and McGinnis (2001) showed that in culture media containing glucose, phosphate inhibited embryo development to blastocyst stage. On the other hand, according to our study, defined medium containing the both glucose and phosphate has exhibited better embryo development compared to glucose alone in undefined media.…”

Section: Discussionmentioning

confidence: 98%

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented with 10 % FBS and defined PVA-myo-inositol-phosphate-EGF medium. No (P [ 0.05) differences at 2-cell, 4-cell and 8-cell to 16-cell stages were observed among the three cultured media used, however, increased (P \ 0.05) blastocyst yield, cell number and hatching rate were found in defined medium compared to undefined media. The expression patterns of genes implicated in embryo metabolism (GLUT-1), anti-apoptosis (BCL-2), imprinting (IGF-2R), DNA methylation (DNMT-3A) and maternal recognition of pregnancy (IFNT) were increased (P \ 0.05) in hatched blastocysts derived from defined medium compared to undefined media. In conclusion, serum-free, defined medium improved developmental competence of in vitro cultured buffalo embryos. Whether these differences in morphological development and gene expression have long-term effects on buffalo calves born after embryo transfer remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development.

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Section: Discussionmentioning

confidence: 98%

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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“…Such irradiation doses contribute to the number of non lethal stress factors related to in vitro culture, according to the data from [10,14], possibly through stress on the respiratory chain. In a review [17] it is discussed how cultured embryos exhibit reduced oxidative capacity during in vitro culture, and hypothesized that the mitochondria are damaged by free radicals at ambient oxygen concentration. It is an interesting hypothesis that blue light and high ambient oxygen act in concert on the mitochondria and result in a reduced oxidative capacity of the developing pre-implantation embryo during in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

Light exposure of the ovum and preimplantation embryo during ART procedures

Ottosen

Hindkjær

Ingerslev

2007

J Assist Reprod Genet

113

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Exposure to visible light (400-700 nm wavelengths) is an unnatural stress factor to preimplantation embryos cultured in vitro. This study investigated the spectral composition and intensity of light during IVF procedures, and calculated radiation doses reaching the embryo during handling and manipulation. The study shows that normal IVF procedure may result in stressing radiation doses, unless filters are applied. This is at present not sufficiently recognised. No Danish IVF clinics use filters to protect embryos against visible light. 95% of the radiation was from microscopes. Ambient light, in contrast, was not a significant contributor to light stress and the use of dark laboratories is not justified.

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“…Thus, the discrepancy might be attributable to the difference in the developmental stages of embryos at the initiation of culture (4-to 8-cell embryos vs. zygotes). It is also possible that the efficacy of taurine may vary according to the composition of the basal medium employed, since taurine can serve not only as an oxidant or free radical scavenger, but also serve as an osmolyte or chelating agent [1,2,9]. Alternatively, taurine might modulate the beneficial effects of insulin that enhances bovine embryonic development by stimulating amino acids transport [16,18].…”

Section: Discussionmentioning

confidence: 99%

“…Recent attention has been focused on the effects of specific substances such as energy substrates, amino acids and growth factors. It has been demonstrated that exogenous supplementation with amino acids is important for the development of mammalian embryos [1,2]. Glutamine, glycine and taurine have been found to be particularly important for the in vitro development of hamster embryos [1,2].…”

Section: Source Of Zygotesmentioning

confidence: 99%

“…It has been demonstrated that exogenous supplementation with amino acids is important for the development of mammalian embryos [1,2]. Glutamine, glycine and taurine have been found to be particularly important for the in vitro development of hamster embryos [1,2]. Glutamine is preferentially metabolized by bovine embryos during the 2-to 4-cell stages [25].…”

Section: Source Of Zygotesmentioning

confidence: 99%

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Effects of Glutamine, Glycine and Taurine on the Development of In Vitro Fertilized Bovine Zygotes in a Chemically Defined Medium.

Takahashi

Kanagawa

1998

The Journal of Veterinary Medical Science

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ABSTRACT. The effects of glutamine, glycine and taurine on the development of bovine zygotes derived from IVM/IVF were determined using a protein-free chemically defined medium. After the cumulus cells were removed at 18 hr post-insemination, the presumptive zygotes were cultured for 4 or 6 days (about 104 or 154 hr) under a gas atmosphere of 5% O 2 : 5% CO 2 : 90% N 2 . A modified synthetic oviduct fluid medium supplemented with 20 amino acids (1 mM glutamine, essential amino acids for basal medium Eagle and nonessential amino acids for minimum essential medium), insulin, and PVA was used as a basic medium (mSOFai). Omitting 1 mM glutamine from mSOFai did not affect the embryonic development after 4 and 6 days of culture. After 4 days of culture, no significant effects of glycine and taurine on the development of zygotes to the morula stage were observed. However, supplementation with glycine or taurine significantly (P<0.05) affected, with no interaction, the embryonic development to blastocysts after 6 days of culture. Addition of 5 mM glycine and 2 or 10 mM taurine significantly (P<0.05) increased the percentage of blastocysts. The mean cell number in the blastocysts was affected by the glycine level, and was increased by the addition of 10 mM glycine (P<0.001). These results demonstrate that glycine and taurine in a chemically defined medium containing a group of essential and non-essential amino acids improve the development of bovine zygotes to the blastocyst stage under 5% O 2 . -KEY WORDS: bovine embryo, culture, glutamine, glycine, taurine.

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What is the relationship between the metabolism of preimplantation embryos and their developmental competence?

Cited by 139 publications

References 246 publications

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

Light exposure of the ovum and preimplantation embryo during ART procedures

Effects of Glutamine, Glycine and Taurine on the Development of In Vitro Fertilized Bovine Zygotes in a Chemically Defined Medium.

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