Objective: Information about full-digital protocols for bite registration with intraoral scanners on multiple implants in the edentulous jaw is scarce. The purpose of this comparative in vivo study was to investigate the reliability and time efficiency of a novel full-digital bite registration technique for the manufacture of full-arch maxillary fixed implant prostheses. Material and methods: In ten patients, a full-arch maxillary fixed implant prosthesis was manufactured on multi-unit abutment level through an analog prosthetic workflow. The bite registration was performed with use of a screw-retained polymethyl methacrylate (PMMA) verification jig with detachable wax rim. To articulate the definitive edentulous maxillary implant cast in centric relation at the appropriate occlusal vertical dimension (OVD) to the mandibular antagonist cast, a type II articulator (Artex, Amann Girrbach) was used. Three to six months later, a full-digital bite registration was performed with use of dual-function scan bodies and bilateral connected bite pillars. The bite pillars screwed into the scan bodies were used to adjust and articulate the edentulous maxillary implant arch to the mandibular antagonist arch at the defined OVD. Treatment time for analog and digital bite registration technique was measured in each patient. The reliability of the digital bite registration technique was evaluated by 3D comparison of two sets of stereo lithographic (STL) files obtained from each patient. The three-dimensional deviation was defined along the X-, Y- and Z-axes (Geomagic Control X, 3D Systems Inc., Rock Hill, SC, USA). Results: The treatment time for digital bite registration using dual-function scan bodies and bite pillars was significantly shorter than analog bite registration with verification jig and wax rim (60.30%, SD 5.72%). Minor differences between the two techniques were observed with a linear deviation range of 1115 µm (SD 668 µm) overall, 46.2 µm (SD 731.3 µm) along the X-axis, −200.3 µm (SD 744.3 µm) along the Y-axis and 67.1 µm (SD 752.2 µm) along the Z-axis. Bilateral balanced contacts were registered in all patients during full-digital bite registration. Conclusions: The novel digital bite registration technique with dual-function scan bodies and bite pillars allows for a full-digital workflow for full-arch implant supported restorations. The digital bite workflow was 60% faster, and the overall deviation was around 1 mm, which can be considered clinically acceptable.