What restricts the activity of mariner-like transposable elements?

Hartl, Daniel L.; Lozovskaya, Elena R.; Nurminsky, Dmitry I.; Lohe, Allan R.

doi:10.1016/s0168-9525(97)01087-1

Cited by 130 publications

(92 citation statements)

References 32 publications

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“…The relationship between the 4.2-and 3.8-kb products might provide a clue to the origin and movement of polymorphic types of BmMLE. According to the recent understanding of the natural history of MLE (Hartl et al, 1997), vertical inactivation and stochastic loss are the long term fate of MLEs in the genome. Thus, most BmMLEs would be in the inactivated stage as found in many MLEs in other organisms.…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of a mariner-like element in the silkworm, Bombyx mori.

Tomita¹,

Sohn

Tamura³

1997

Genes Genet. Syst.

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We have identified a mariner-like element (MLE) in the genome of the silkworm, Bombyx mori. The cloned MLE, desiqnated as BmMLE, contained an ORF that was interrupted by insertions, stop codons, and frameshifts. The complete ORF sequence was estimated by sequencing the PCR products. The hypothetical protein coded by this ORF showed 34% homology to the Mos1 element of Drosophila mauritiana, and is phylogenetically very close to MLE of Hyalophora cecropia. BmMLE is present in 80 to 100 copies in the B. mori genome. Many BmMLEs with different lengths were identified by Southern blot analysis and LA PCR of the genomic DNA. The distribution of these BmMLEs differed in geographic races.

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Section: Discussionmentioning

confidence: 99%

Cloning and characterization of a mariner-like element in the silkworm, Bombyx mori.

Tomita¹,

Sohn

Tamura³

1997

Genes Genet. Syst.

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“…It is a widely accepted idea from the viewpoint of evolution that natural selection acts against high activity of transposable elements because of their deleterious effects on their host organisms (Hartl et al 1997). Tol1 elements of substrain A appear to have been stabilized as a result of natural selection, like most of the transposable elements found in various organisms.…”

Section: Possible Cause Of Resumption Of Transpositionmentioning

confidence: 99%

The Tol1 transposable element of the medaka fish moves in human and mouse cells

et al. 2007

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DNA-based transposable elements can be used as tools for gene engineering and gene therapy. A great advantage over RNA-mediated elements and retroviruses is the simplicity and safety of usage. The Tol1 element of the medaka fish Oryzias latipes has structural features of DNA-based elements. Although its excision has already been demonstrated, de novo insertion has not been observed, and a transposase has not been hitherto identified. We first cloned, through in silico search alignments and genomic library screenings, a 4.4-kb Tol1 copy carrying open reading frames and then identified, by mRNA analysis, a 2.9-kb transcript coding for 851 amino acids. The protein product of this transcript catalyzed transposition of a nonautonomous Tol1 copy in human and mouse culture cells. This identification of a fully functional Tol1 transposase could lead to the development of new tools for basic and translational molecular biology applications in mammals.

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“…Our explanation for the presence of several mammal mar1 populations in the mammalian genomes appears to be parsimonious and minimizes horizontal transfers as previously recommended in the literature (Capy et al 1996;Hartl et al 1997). However, it does not answer a controversial observation obtained by comparing the invertebrate and mammalian MLEs related to the cecropia sub-family.…”

Section: Discussionmentioning

confidence: 65%

Features of the mammal mar1 transposons in the human, sheep, cow, and mouse genomes and implications for their evolution

Demattei¹,

Augé-Gouillou²,

Pollet

et al. 2000

Mammalian Genome

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Abstract. Mariner-like elements (MLE) belong to the Tc1/ mariner superfamily of class II transposons. We have analyzed the mariner related to the cecropia subfamily, and called mammal mar1, in four mammalian genomes, Bos taurus (Bovidae), Homo sapiens (Primata), Mus musculus (Rodentia), and Ovis aries (Ovidae). Three kinds of MLE sequences were found in all these species: full-length 1.3-kbp elements, shorter elements 80 bp-1.2 kbp, and single inverted terminal repeats (ITRs). All the 1.3-kbp genomic copies sequenced had an open reading frame encoding a transposase interrupted by stop codons or frame shifts. Phylogenetic analysis of the full-length elements suggested at least two distinct populations of mammal mar1 elements in each species. This was confirmed by using a statistical method that allows defining populations. Finally, the evolutionary origin of the mammal mar1 elements and the paradoxes are discussed.

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What restricts the activity of mariner-like transposable elements?

Cited by 130 publications

References 32 publications

Cloning and characterization of a mariner-like element in the silkworm, Bombyx mori.

Cloning and characterization of a mariner-like element in the silkworm, Bombyx mori.

The Tol1 transposable element of the medaka fish moves in human and mouse cells

Features of the mammal mar1 transposons in the human, sheep, cow, and mouse genomes and implications for their evolution

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