What’s for Dinner?: Entner-Doudoroff Metabolism in
            <i>Escherichia coli</i>

Peekhaus, Norbert; Conway, Tyrrell

doi:10.1128/jb.180.14.3495-3502.1998

Cited by 263 publications

(155 citation statements)

References 63 publications

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“…The edd gene codes for 6-phosphogluconate dehydratase, an enzyme that catalyses the first step in the Entner-Doudoroff (ED) pathway (Wanken et al, 2003) which comprises the dehydration of 6-phospho-D-gluconate into 6phospho-2-dehydro-3-deoxy-D-gluconate (Peekhaus and Conway, 1998;Kim et al, 2007). Many bacteria possess genes for the ED pathway (Kim et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide analysis of bacterial determinants of plant growth promotion and induced systemic resistance by Pseudomonas fluorescens

Etalo

Mortel

et al. 2017

Environmental Microbiology

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Pseudomonas fluorescens strain SS101 (Pf.SS101) promotes growth of Arabidopsis thaliana, enhances greening and lateral root formation, and induces systemic resistance (ISR) against the bacterial pathogen Pseudomonas syringae pv. tomato (Pst). Here, targeted and untargeted approaches were adopted to identify bacterial determinants and underlying mechanisms involved in plant growth promotion and ISR by Pf.SS101. Based on targeted analyses, no evidence was found for volatiles, lipopeptides and siderophores in plant growth promotion by Pf.SS101. Untargeted, genome-wide analyses of 7488 random transposon mutants of Pf.SS101 led to the identification of 21 mutants defective in both plant growth promotion and ISR. Many of these mutants, however, were auxotrophic and impaired in root colonization. Genetic analysis of three mutants followed by site-directed mutagenesis, genetic complementation and plant bioassays revealed the involvement of the phosphogluconate dehydratase gene edd, the response regulator gene colR and the adenylsulfate reductase gene cysH in both plant growth promotion and ISR. Subsequent comparative plant transcriptomics analyses strongly suggest that modulation of sulfur assimilation, auxin biosynthesis and transport, steroid biosynthesis and carbohydrate metabolism in Arabidopsis are key mechanisms linked to growth promotion and ISR by Pf.SS101.

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Section: Discussionmentioning

confidence: 99%

Genome‐wide analysis of bacterial determinants of plant growth promotion and induced systemic resistance by Pseudomonas fluorescens

Etalo

Mortel

et al. 2017

Environmental Microbiology

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“…Unexpectedly, L. pneumophila seem to exploit host carbohydrates in addition to proteins as energy sources, as components of the Entner-Doudoroff (E-D) pathway were upregulated by replicating Legionella. This NADPH producing mechanism is commonly used for sugar and/or gluconate assimilation (Peekhaus and Conway, 1998) and was also shown to be upregulated by Salmonella enterica growing in macrophages (Eriksson et al, 2003). Utilization of the E-D pathway may be used for the generation of NADPH from endogenous glucose or glucose-6-phosphate.…”

Section: Discussionmentioning

confidence: 99%

Virulence strategies for infecting phagocytes deduced from the in vivo transcriptional program of Legionella pneumophila

et al. 2006

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SummaryAdaptation to the host environment and exploitation of host cell functions are critical to the success of intracellular pathogens. Here, insight to these virulence mechanisms was obtained for the first time from the transcriptional program of the human pathogen Legionella pneumophila during infection of its natural host, Acanthamoeba castellanii . The biphasic life cycle of L. pneumophila was reflected by a major shift in gene expression from replicative to transmissive phase, concerning nearly half of the genes predicted in the genome. However, three different L. pneumophila strains showed similar in vivo gene expression patterns, indicating that common regulatory mechanisms govern the Legionella life cycle, despite the plasticity of its genome. During the replicative phase, in addition to components of aerobic metabolism and amino acid catabolism, the Entner-Doudoroff pathway, a NADPH producing mechanism used for sugar and/or gluconate assimilation, was expressed, suggesting for the first time that intracellular L. pneumophila may also scavenge host carbohydrates as nutrients and not only proteins. Identification of genes only upregulated in vivo but not in vitro , may explain higher virulence of in vivo grown L. pneumophila. Late in the life cycle, L. pneumophila upregulates genes predicted to promote transmission and manipulation of a new host cell, therewith priming it for the next attack. These including substrates of the Dot/Icm secretion system, other factors associated previously with invasion and virulence, the motility and the type IV pilus machineries, and > 90 proteins not characterized so far. Analysis of a fliA ( s 28 ) deletion mutant identified genes coregulated with the flagellar regulon, including GGDEF/EAL regulators and factors that promote host cell entry and survival.

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“…The hrmA product has no significant sequence similarity in the entire database as of October, 2002. Except for HrmE, the known proteins are characteristic of gluconate, glucuronate and galacturonate catabolism in Escherichia coli (Lin, 1996;Peekhaus and Conway, 1998) and Bacillus species (Mekjian et al ., 1999;Shulami et al ., 1999). The genes in this locus of the N .…”

Section: Resultsmentioning

confidence: 99%

“…This observation is also consistent with results indicating that HrmR does not regulate the transcription of the hrmI, hrmU, or hrmA genes in the context of regulon or operon structure; the HrmR binding sequence is absent in the 5¢ regions of those genes and Northern hybridization bands are consistent with primarily co-transcription of hrmUA and monocistronic hrmI transcription (data not shown). In this respect, transcription in the hrm locus of N. punctiforme differs from the regulon structure and polycistronic expression of hexuronic acid catabolic genes in E. coli (Peekhaus and Conway, 1998) and Bacillus species (Mekjian et al, 1999;Shulami et al, 1999). Transcriptional regulation in the hrm locus is complex and appears to involve different signal molecules and mechanisms, one of which is sugar-binding dependent derepression.…”

Section: Discussionmentioning

confidence: 99%

DNA binding properties of the HrmR protein of Nostoc punctiforme responsible for transcriptional regulation of genes involved in the differentiation of hormogonia

Campbell

Wong

Meeks³

2003

Molecular Microbiology

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SummaryNostoc punctiforme is an example of a filamentous cyanobacterium that is capable of differentiating nongrowing cells that constitute gliding filaments termed hormogonia. These gliding filaments serve in short distance dispersal and as infective units in establishing a symbiosis with plants, such as the bryophyte Anthoceros punctatus . Mutants of N . punctiforme exist which show elevated levels of initial infection of A . punctatus as a consequence of repeated cycles of hormogonium differentiation. Such mutations occur within the hrmA and hrmU genes. Further characterization of the hrm locus revealed several genes with an organizational and predicted protein sequence similarity to genes of heterotrophic bacteria that are involved in hexuronic acid metabolism. Genes in the N. punctiforme locus are transcribed in response to the presence of a plant extract containing hormogonium-repressing factors. A predicted transcriptional repressor encoded in the locus, HrmR , was shown herein to be a specific DNA binding protein that regulates the transcription of its own gene and that of hrmE , a nearby gene. The ability of HrmR to bind DNA was abolished upon addition of either galacturonate or lysate from specifically induced N . punctiforme cells, implying that the in vivo HrmR binding activity is modulated via an internal compound, most likely a sugar molecule.

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What’s for Dinner?: Entner-Doudoroff Metabolism in Escherichia coli

Cited by 263 publications

References 63 publications

Genome‐wide analysis of bacterial determinants of plant growth promotion and induced systemic resistance by Pseudomonas fluorescens

Genome‐wide analysis of bacterial determinants of plant growth promotion and induced systemic resistance by Pseudomonas fluorescens

Virulence strategies for infecting phagocytes deduced from the in vivo transcriptional program of Legionella pneumophila

DNA binding properties of the HrmR protein of Nostoc punctiforme responsible for transcriptional regulation of genes involved in the differentiation of hormogonia

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