What’s new in flow biocatalysis? A snapshot of 2020–2022

Crotti, Michele; Robescu, Marina Simona; Bolívar, Juan M.; Ubiali, Daniela; Wilson, Lorena; Contente, Martina Letizia

doi:10.3389/fctls.2023.1154452

Cited by 9 publications

(2 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therein, the total CO 2 production decreases considerably, since resources are more efficiently used (more substrate per solvent volume) [ 8 , 16 ]. As a clear conclusion, the need for process intensification appears mandatory to reach truly sustainable processes [ 25 , 30 , 31 , 32 ] ( Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

Biocatalysis in Water or in Non-Conventional Media? Adding the CO2 Production for the Debate

Domínguez de María,

Kara,

Gallou

2023

Molecules

View full text Add to dashboard Cite

Biocatalysis can be applied in aqueous media and in different non-aqueous solutions (non-conventional media). Water is a safe solvent, yet many synthesis-wise interesting substrates cannot be dissolved in aqueous solutions, and thus low concentrations are often applied. Conversely, non-conventional media may enable higher substrate loadings but at the cost of using (fossil-based) organic solvents. This paper determines the CO2 production—expressed as kg CO2·kg product−1—of generic biotransformations in water and non-conventional media, assessing both the upstream and the downstream. The key to reaching a diminished environmental footprint is the type of wastewater treatment to be implemented. If the used chemicals enable a conventional (mild) wastewater treatment, the production of CO2 is limited. If other (pre)treatments for the wastewater are needed to eliminate hazardous chemicals and solvents, higher environmental impacts can be expected (based on CO2 production). Water media for biocatalysis are more sustainable during the upstream unit—the biocatalytic step—than non-conventional systems. However, processes with aqueous media often need to incorporate extractive solvents during the downstream processing. Both strategies result in comparable CO2 production if extractive solvents are recycled at least 1–2 times. Under these conditions, a generic industrial biotransformation at 100 g L−1 loading would produce 15–25 kg CO2·kg product−1 regardless of the applied media.

show abstract

Section: Resultsmentioning

confidence: 99%

Biocatalysis in Water or in Non-Conventional Media? Adding the CO2 Production for the Debate

Domínguez de María,

Kara,

Gallou

2023

Molecules

View full text Add to dashboard Cite

show abstract

“…To overcome this issue, enzyme immobilization emerges as a great solution to enhance enzyme stability and allows enzyme recycling once the reaction is completed. , Moreover, immobilized enzymes present greater versatility to be implemented in different types of reactors. Not surprisingly, enzyme immobilization is the key enabling technology revolutionizing flow biocatalysis. − …”

Section: Introductionmentioning

confidence: 99%

Optimized Spatial Configuration of Heterogeneous Biocatalysts Maximizes Cell-Free Biosynthesis of ω-Hydroxy and ω-Amino Acids

Santiago-Arcos,

Velasco-Lozano,

Diamanti

et al. 2024

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Cell-free biocatalysis is gaining momentum in producing value-added chemicals, particularly in stepwise reaction cascades. However, the stability of enzyme cascades in industrial settings is often compromised when free enzymes are involved. In this study, we have developed a stable multifunctional heterogeneous biocatalyst coimmobilizing five enzymes on microparticles to transform 1,ω-diols into 1,ω-hydroxy acids. We improved the operational efficiency and stability of the heterogeneous biocatalyst by fine-tuning the enzyme loading and spatial organization. Stability issues are overcome through postimmobilization polymer coating. The general applicability of this heterogeneous biocatalyst is demonstrated by its scale-up in both batch and packed bed reactors, allowing a product yield of >80%. The continuous process is fed with H 2 O 2 as the oxygen source, reaching a space-time yield (STY) of 0.76 g•L −1 •h −1 , maintained for the first 12 h. Finally, this flow system is telescoped with a second plug-flow reactor packed with a different heterogeneous biocatalyst integrating an additional transaminase. As a result, this 6-enzyme 2-reactor system sequentially transforms 1,ω-diols into 1,ω-amino acids while in situ recycling NAD + , depleting H 2 O 2 , and generating O 2 .

show abstract

Perspectives on flow biocatalysis: the engine propelling enzymatic reactions

Benítez-Mateos,

Paradisi

2023

J Flow Chem

View full text Add to dashboard Cite

Flow biocatalysis has emerged as an empowering tool to boost the potential of enzymatic reactions towards more automatized, sustainable, and generally efficient synthetic processes. In the last fifteen years, the increasing number of biocatalytic transformations carried out in continuous flow exemplified the benefits that this technology can bring to incorporate biocatalysis into industrial operations. This perspective aims to capture in a nutshell the available methodologies for flow biocatalysis as well as to discuss the current limitations and the future directions in this field. Graphical abstract

show abstract

What’s new in flow biocatalysis? A snapshot of 2020–2022

Cited by 9 publications

References 107 publications

Biocatalysis in Water or in Non-Conventional Media? Adding the CO2 Production for the Debate

Biocatalysis in Water or in Non-Conventional Media? Adding the CO2 Production for the Debate

Optimized Spatial Configuration of Heterogeneous Biocatalysts Maximizes Cell-Free Biosynthesis of ω-Hydroxy and ω-Amino Acids

Perspectives on flow biocatalysis: the engine propelling enzymatic reactions

Contact Info

Product

Resources

About