What's New in Light and Electron Microscopic Immunocytochemistry? Application of the Protein A-Gold Technique to Routinely Processed Tissue

Roth, Jürgen; Kasper, M.; Heitz, Ph. U.; Labat, Françoise

doi:10.1016/s0344-0338(85)80054-6

Cited by 8 publications

(2 citation statements)

References 30 publications

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“…Ultrathin Epon sections from glutaraldehyde-osmium tetroxidefixed tissue mounted on nickel grids were placed on droplets of freshly prepared 1% aqueous periodic acid for 4-6 min at ambient temperature and thereafter thoroughly rinsed for 30 min by passing them over a series of droplets of distilled water (Roth et al 1985(Roth et al , 2000. Sections were then conditioned with PBS containing 1% BSA, 0.01% Triton X-100, and 0.01% Tween 20 for 10 min at ambient temperature and transferred to droplets of guinea pig antirat C-peptide antibodies for 2 h at ambient temperature.…”

Section: Immunoelectron Microscopymentioning

confidence: 99%

Ultrastructural analysis of transitional endoplasmic reticulum and pre-Golgi intermediates: a highway for cars and trucks

Fan

Roth

Zuber

2003

Histochemistry and Cell Biology

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Cargo selection and export from the endoplasmic reticulum occurs at specialized sites in cells. Export complexes consist of transitional elements of the endoplasmic reticulum and pre-Golgi intermediates. It is generally assumed that 60 to 80 nm initially COPII-coated vesicles derived from the transitional endoplasmic reticulum are the main carriers for transport of cargo to the Golgi apparatus. We have analyzed on serial ultrathin sections the transitional endoplasmic reticulum and pre-Golgi intermediates of beta cells of islets of Langerhans in mouse pancreas. In addition to Golgi-associated complexes, others were observed in the periphery of the cells or close to the nuclear envelope. Upon three-dimensional reconstruction, non-coated ribosome-free tubules with an average diameter of 115 nm (range 60-195 nm) and a length of up to 500 nm were detected in the pre-Golgi intermediates in addition to small vesiculo-tubular elements. Furthermore, evidence was found that the large tubular elements may directly arise from transitional elements of the endoplasmic reticulum. In a given cell, pre-Golgi intermediates were found to be composed solely of small vesiculo-tubular elements or additionally of tubules or solely of tubules. Immunogold labeling for proinsulin indicated that the large tubular elements contained cargo and thus appear to take part in ER-to-Golgi transport.

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Section: Immunoelectron Microscopymentioning

confidence: 99%

Ultrastructural analysis of transitional endoplasmic reticulum and pre-Golgi intermediates: a highway for cars and trucks

Fan

Roth

Zuber

2003

Histochemistry and Cell Biology

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“…Retrospective studies on aldehyde/osmium-fixed and Epon-embedded tumors may be possible by section pretreatment with sodium metaperiodate (Bendayan and Zollinger 1983) or periodic acid (Figs. 1, 2) (Roth et al 1985).…”

Section: Tissue Fixation and Embeddingmentioning

confidence: 99%

Applications of immunogold and lectin-gold labeling in tumor research and diagnosis

Roth

Zuber

Komminoth

et al. 1996

Histochem Cell Biol

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Immunohistochemistry and carbohydrate histochemistry have had an enormous impact on both tumor research and diagnosis. In particular, immunogold labeling has provided significant advantages over classical fluorescence and enzyme-based techniques. In light microscopy, the silver-intensified gold labeling has proven highly sensitive and precise in localization. In electron microscopy, the gold particle marker was a prerequisite for successful and unequivocal antigen detection in electron-dense cellular structures such as secretory granules. In this review we demonstrate the usefulness of light and electron microscopical gold labeling techniques as applied in tumor research and diagnosis. The examples include expression of beta-1,6 branches and specific sialoglycoconjugates in colon carcinoma, b-12 carbohydrate epitope in breast carcinoma, polysialic acid in neuroendocrine tumors of lung, adrenal and thyroid, as well as studies on proinsulin to insulin conversion in insulinomas. In addition, practical hints for prevention of background staining, tissue fixation, and silver intensification of gold labeling are given.

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Localization of Hormones by Antibodies

Heitz

1990

Hormone-Related Malignant Tumors

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What's New in Light and Electron Microscopic Immunocytochemistry? Application of the Protein A-Gold Technique to Routinely Processed Tissue

Cited by 8 publications

References 30 publications

Ultrastructural analysis of transitional endoplasmic reticulum and pre-Golgi intermediates: a highway for cars and trucks

Ultrastructural analysis of transitional endoplasmic reticulum and pre-Golgi intermediates: a highway for cars and trucks

Applications of immunogold and lectin-gold labeling in tumor research and diagnosis

Localization of Hormones by Antibodies

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