What tissue bankers should know about the use of allograft blood vessels

Dodd, P.D.F.

doi:10.1007/s10561-009-9149-9

Cited by 13 publications

(7 citation statements)

References 41 publications

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“…Allogeneic blood vessels play an important role in infected cardiac and vascular surgery [Nevelsteen et al, 1998;Leseche et al, 2001;Gabriel et al, 2004;Vardanian et al, 2009;Bisdas et al, 2010;Dodd, 2010]. The current state of the art for preserving human cardiovascular tissues, including arteries, is the conventional freezing cryopreservation (CFC) method, employing controlled-rate freezing with 10-20% dimethylsulfoxide (DMSO).…”

Section: Introductionmentioning

confidence: 99%

Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility

Huber¹,

Brockbank²,

Riemann

et al. 2012

Cells Tissues Organs

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Objective: Arterial allografts are routinely employed for reconstruction of infected prosthetic grafts. Usually, banked cryopreserved arteries are used; however, existing conventional freezing cryopreservation techniques applied to arteries are expensive. In contrast, a new ice-free cryopreservation technique results in processing, storage and shipping methods that are technically simpler and potentially less costly. The objective of this study was to determine whether or not ice-free cryopreservation causes tissue changes that might preclude clinical use. Methods: Conventionally frozen cryopreserved porcine arteries were compared with ice-free cryopreserved arteries and untreated fresh controls using morphological (light, scanning electron and laser scanning microscopy), viability (alamarBlue assay) and hemocompatibility methods (blood cell adhesion, thrombin/antithrombin-III-complex, polymorphonuclear neutrophil-elastase, β-thromboglobulin and terminal complement complex SC5b-9). Results: No statistically significant structural or hemocompatibility differences between ice-free cryopreserved and frozen tissues were detectable. There were no quantitative differences observed for either autofluorescence (elastin) or second harmonic generation (collagen) measured by laser scanning microscopy. Cell viability in ice-free cryopreserved arteries was significantly reduced compared to fresh and frozen tissues (p < 0.05). Conclusions: The formation of ice in aortic artery preservation did not make a difference in histology, structure or thrombogenicity, but significantly increased viability compared with a preservation method that precludes ice formation. Reduced cell viability should not reduce in vivo performance. Therefore, ice-free cryopreservation is a potentially safe and cost-effective technique for the cryopreservation of blood vessel allografts.

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Section: Introductionmentioning

confidence: 99%

Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility

Huber¹,

Brockbank²,

Riemann

et al. 2012

Cells Tissues Organs

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“…2 Other surgical approaches reported in the literature to restore arterial flow include the use of antibiotic impregnated surgical conduit grafts, the use of autologous superficial femoral vein grafts, endovascular stent grafting and the use of cadaveric arterial aortic allografts. 3 In this case, we felt the large intraabdominal septic burden and the patient's relatively young age (42 years) were reasons to try and avoid extra-anatomic bypass grafting if at all possible. Furthermore, the presence of bilateral DVTs precluded an attempt at using superficial veins for aortic reconstruction.…”

Section: Discussionmentioning

confidence: 88%

“…Although we undertake routine endovascular stent grafting for atherosclerotic aortic aneurysms in our unit, we were reluctant to consider this approach for fear of introducing foreign material into the aneurysm, particularly as endovascular stent grafting for MAAAs has been reported as only to be a potential bridge to open surgery. 3 Cryopreserved arterial allografts have been adopted successfully as a treatment for MAAAs in mainland Europe and the US. Allografts are retrieved from deceased donors and assessed against rejection criteria before being stored frozen indefinitely.…”

Section: Discussionmentioning

confidence: 99%

“…1,4,5 Until recently, there was no UK provision of vascular allografts for intra-abdominal aortic and peripheral arterial surgery (as opposed to sections of aortic root and valves for cardiac surgery) but the NHS tissue bank started issuing SFA allografts in early 2009. 3 Currently, SFA allografts are the only type of arterial allograft issued by the NHS tissue bank. Sections of infrarenal aorta are not available.…”

Section: Discussionmentioning

confidence: 99%

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Novel surgical reconstruction of a mycotic abdominal aortic aneurysm using cryopreserved femoral arterial allograft from the NHS tissue bank: a new resource for UK vascular surgeons

Abbas

Davies

Kabir

et al. 2012

annals

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The treatment of mycotic abdominal aortic aneurysms remains a significant surgical challenge associated with significant morbidity and mortality. In the following case report, we describe our successful management of a patient with a mycotic abdominal aortic aneurysm using two cryopreserved superficial femoral artery allografts (available from the UK NHS tissue bank) to create a Y-shaped allograft to permit immediate aortic reconstruction after surgical debridement. To our knowledge, this is the first time such a reconstruction has been reported in the literature. Case historyA 42-year-old man was admitted acutely to the medical admissions unit complaining of abdominal pain. He had a history of intravenous drug abuse, previous groin abscesses secondary to infected injection sites and bilateral lower limb deep vein thromboses (DVTs). He was pyrexial on admission and abdominal examination revealed a central nontender pulsatile mass. There was no evidence of any groin sepsis but both legs were swollen. Blood results revealed normocytic normochromic anaemia (Hb 9.3g/l) and raised inflammatory markers (WCC: 16.7 x 10 9

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“…Meanwhile the authors reported the superiority of the method when compared to venous graft reconstruction due to the fact that arterial grafts have a lower risk of developing postoperative complications such as thrombosis, stenosis or infection (22). Therefore, it seems that arterial grafts are more resistant to surgical manipulation, do not have valves, are more effective in reducing the occlusion risk due to external visceral compression and are associated with a lower risk of graft infection (23,24).…”

Section: Discussionmentioning

confidence: 99%

Portal vein reconstruction with interposition of cryopreserved aortic graft: A case report and literature review

Bacalbaşa

Bălescu²,

Diaconu

et al. 2021

Exp Ther Med

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Pancreatic cancer is one of the most aggressive malignancies with poor rates of survival especially in the event radical procedures are not feasible. However, improvements in surgical techniques have led to the successful association of vascular resection followed by reconstruction without a significant increase in the rates of postoperative complications. In the present article, we present the case of a 49-year-old patient diagnosed with pancreatic head cancer invading the portal vein. After discussing with the patient the risks and the benefits of the surgical procedure, the patient was submitted to pancreatoduodenectomy en bloc with portal vein resection while the continuity of the portal vein was reestablished by using a cadaveric graft originating from the abdominal aorta. The postoperative outcome was uneventful. In conclusion, in selected cases, arterial cadaveric grafts may be used in order to establish the continuity of the portal vein with good results. However, it should be emphasized that these are demanding procedures which should be carefully analyzed before deciding upon the opportunity for performing them.

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What tissue bankers should know about the use of allograft blood vessels

Cited by 13 publications

References 41 publications

Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility

Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility

Novel surgical reconstruction of a mycotic abdominal aortic aneurysm using cryopreserved femoral arterial allograft from the NHS tissue bank: a new resource for UK vascular surgeons

Portal vein reconstruction with interposition of cryopreserved aortic graft: A case report and literature review

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