The American Chemical Society, Division of Chemical Education, Examinations Institute has been developing content maps for the undergraduate program based on subdiscipline specifications since 2008. The Anchoring Concepts Content Maps (or ACCM) have been published in four subdisciplines (general, organic, physical, and inorganic chemistry) with the latest work presented here in a fifth subdiscipline: analytical chemistry. As with the development of the other maps, an alignment process of identifying key components of exam items including content, complexity, and other item characteristics was conducted with both analytical chemistry and instrumental analysis items. The results of the alignments as well as an evaluation of the newest ACCM in relation to the other published maps are presented.