Wheat doubled haploids have a marked prevalence of chromosomal aberrations

Shrestha, Sandesh; Koo, Dal‐Hoe; Evers, Byron; Wu, Shuangye; Walkowiak, Sean; Hucl, P.; Pozniak, Curtis; Fritz, Allan K.; Poland, Jesse

doi:10.1002/tpg2.20309

Cited by 5 publications

(3 citation statements)

References 70 publications

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“…To the best of our knowledge, our study is the first to report genomic instability in a B. carinata genotype: this was unexpected due to the wider genetic divergence between the Brassica B and C genomes relative to the A and C genomes (Parkin et al 1995, Lagercrantz and Lydiate 1996, Perumal et al 2020). It is possible that this finding relates to the fact that this B. carinata genotype is derived from microspore culture to produce doubled-haploid lines, which may confer genomic instability (Shrestha et al 2023). However, as both B. carinata genotypes used in this study are derived from the same microspore culture process (also at the same time point with the same protocol), there may also be variation for non-homologous chromosome recombination frequency within genotypes of the parent allotetraploid species, as has been hypothesized on the basis of cytological results for B. napus genotypes (Sheidai et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Karyotype instability varies by species and genotype combination in allohexaploidBrassica

Quezada-Martinez,

Batley,

Mason

2023

Preprint

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SyntheticBrassicaallohexaploids (2n = AABBCC) do not exist naturally but can be produced between six different parent species combinations, and can be used to investigate processes of polyploid formation and genome stabilization. In this study, we investigated hybridization potential, accumulation and frequency of copy number variants (CNVs), fertility, and karyotype stability in advanced generations of diverse allohexaploid genotypes belonging to differentBrassicaallohexaploid species combinations (NCJ types:B. napus×B. carinata×B. juncea; junleracea types:B. juncea×B. oleracea; naponigra types:B. napus×B. nigra; and carirapa types:B. rapa×B. carinata). Only 3.2% of allohexaploid plants investigated were euploids, with high frequencies of rearrangements. Significant differences between genotypes and between lineages within parent genotype combinations were found for frequencies of euploids and rearrangements, with one NCJ line showing relatively high karyotype stability. Hybridization between different allohexaploids was mostly achievable, with 0 - 4.6 seeds per flower bud on average, and with strong effects of maternal genotype. Novel hybrids between allohexaploid lineages showed similar fertility and stability to their parents. In the novel hybrid population, a significant correlation was observed between the inheritance of A-genome chromosome fragments (relative to C-genome fragments) and the total number of seeds produced per plant (r= 0.24). Our results suggest that syntheticBrassicaallohexaploids can develop genomic stability, but that this occurs at very low frequencies, and may not always be under selective pressure due to the unpredictable relationship between fertility and genome composition in these hybrid types.

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Section: Discussionmentioning

confidence: 99%

Karyotype instability varies by species and genotype combination in allohexaploidBrassica

Quezada-Martinez,

Batley,

Mason

2023

Preprint

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“…Likely, they were caused by colchicine treatment. Shrestha et al [105] studied two wheat DH populations and found many chromosomal aberrations, including duplication, deletion, translocation, and aneuploidy, which were likely caused by unusual chemical exposure during haploid induction and chromosome doubling.…”

Section: Stability Of Doubled Haploidsmentioning

confidence: 99%

Technology for Production of Wheat Doubled Haploid via Maize Pollen Induction—Updated Review

Guan,

Peng,

2024

Agronomy

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Chromosome elimination resulting in haploids is achieved by rapid loss of chromosomes from one parent during the zygote stage and is an important procedure to produce doubled haploid (DH) lines in plants. During crosses between an emasculated wheat (Triticum aestivum L.) and maize (Zea mays L.) as pollen donors, the complete loss of maize chromosomes results in wheat haploid embryos. Through embryo rescue and chromosome doubling processes, pure lines with stable traits can be quickly obtained. The technique is called the “Wheat × Maize System”. Although this technology is not new, it remains a practical approach to date. In order to optimize and improve this technology and to achieve its maximum potential in the winter wheat area of China, this paper reviews the previous and ongoing research and technical procedures for the production of wheat DH lines via the maize pollen induction and presents outlooks on DH research and its application in wheat breeding.

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“…SHGD and colchicine treatments exert stress on the genome of treated plants. The skim sequencing of wheat (Triticum aestivum L.) showed that various chromosomal aberrations, including deletions, duplications, and aneuploidy, can happen due to genomic shock during DH production [20]. These alterations can affect plant fitness and agronomic performance.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous and Chemically Induced Genome Doubling and Polyploidization in Vegetable Crops

Fomicheva,

Kulakov,

Alyokhina

et al. 2024

Horticulturae

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Plant ploidy manipulation is often required for breeding purposes. However, there is no comprehensive review covering genome doubling in vegetable crops despite the abundance of data for a large number of vegetable species. Similar to other species, genome doubling is required in vegetable crops to obtain doubled haploids (DHs). It is also utilized for the production of polyploids to overcome interspecific hybrid sterility and improve agricultural traits. Spontaneous haploid genome duplication (SHGD) occurs in many Apiaceae, Brassicaceae, Cucurbitaceae, and Solanaceae crops, allowing for the laborious treatment with antimitotic agents to be bypassed. SHGD mechanisms are not fully understood, but existing data suggest that SHGD can occur via nuclear fusion, endoreduplication, or other mechanisms during microspore or ovule early embryogenic development. Other studies show that SHGD can occur at later developmental stages during extended plant growth in vitro or ex vitro, possibly due to the presence of phytohormones in the medium and/or diploid cell competitive advantage. For unresponsive accessions and species with rare SHGD, such as onion (Allium cepa L.) and beet cultivars (Beta vulgaris subsp. vulgaris L.), antimitotic agent treatment has to be applied. Antimitotic agent application efficiency depends on the treatment conditions, especially the agent concentration and exposure time. Also, plant developmental stage is critical for agent accessibility and plant survival. The existing methods can be used to further improve genome doubling methodology for major vegetable crops and other species.

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Wheat doubled haploids have a marked prevalence of chromosomal aberrations

Cited by 5 publications

References 70 publications

Karyotype instability varies by species and genotype combination in allohexaploidBrassica

Karyotype instability varies by species and genotype combination in allohexaploidBrassica

Technology for Production of Wheat Doubled Haploid via Maize Pollen Induction—Updated Review

Spontaneous and Chemically Induced Genome Doubling and Polyploidization in Vegetable Crops

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