Wheat Elongator subunit 4 is required for epigenetic regulation of host immune response to Rhizoctonia cerealis

Wang, Kai; Rong, Wei; Liu, Yuping; Li, Hui; Zhang, Zengyan

doi:10.1016/j.cj.2019.11.005

Cited by 9 publications

(12 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate whether TaWAK-6D is required for the expression of defense-related genes in wheat, RT-qPCR was used to examine the transcript levels of wheat defense-related genes in TaWAK-6D -silenced and the BSMV: GFP-infected (control) wheat plants. The tested genes included TaMPK3, TaERF3, Tadefensin, TaPR1, TaChitinase3 , and TaChitinase4 , which have been shown to participate positively in wheat resistance responses to fungal diseases (Zhu et al, 2017 ; Wang K. et al, 2020 ). The analyses showed that the transcript levels of these six genes were significantly decreased in TaWAK-6D -silenced CI12633 plants relative to the BSMV: GFP-infected CI12633 plants ( Figures 6A–F ).…”

Section: Resultsmentioning

confidence: 99%

The Wheat Wall-Associated Receptor-Like Kinase TaWAK-6D Mediates Broad Resistance to Two Fungal Pathogens Fusarium pseudograminearum and Rhizoctonia cerealis

Guo

et al. 2021

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

The soil-borne fungi Fusarium pseudograminearum and Rhizoctonia cerealis are the major pathogens for the economically important diseases Fusarium crown rot (FCR) and sharp eyespot of common wheat (Triticum aestivum), respectively. However, there has been no report on the broad resistance of wheat genes against both F. pseudograminearum and R. cerealis. In the current study, we identified TaWAK-6D, a wall-associated kinase (WAK) which is an encoding gene located on chromosome 6D, and demonstrated its broad resistance role in the wheat responses to both F. pseudograminearum and R. cerealis infection. TaWAK-6D transcript induction by F. pseudograminearum and R. cerealis was related to the resistance degree of wheat and the gene expression was significantly induced by exogenous pectin treatment. Silencing of TaWAK-6D compromised wheat resistance to F. pseudograminearum and R. cerealis, and repressed the expression of a serial of wheat defense-related genes. Ectopic expression of TaWAK-6D in Nicotiana benthamiana positively modulated the expression of several defense-related genes. TaWAK-6D protein was determined to localize to the plasma membrane in wheat and N. benthamiana. Collectively, the TaWAK-6D at the plasma membrane mediated the broad resistance responses to both F. pseudograminearum and R. cerealis in wheat at the seedling stage. This study, therefore, concludes that TaWAK-6D is a promising gene for improving wheat broad resistance to FCR and sharp eyespot.

show abstract

Section: Resultsmentioning

confidence: 99%

The Wheat Wall-Associated Receptor-Like Kinase TaWAK-6D Mediates Broad Resistance to Two Fungal Pathogens Fusarium pseudograminearum and Rhizoctonia cerealis

Guo

et al. 2021

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Seven dpi with R. cerealis , leaf sheathes of BSMV:TaWAK7D and BSMV:GFP-infected CI12633 plants were staining in the Trypan blue solution for 5 min, and then checked whether the hyphaes of R. cerealis infected the wheat plant cells under a microscope. At 20 and 40 dpi, sharp eyespot infection types (ITs) of wheat plants were pictured and scored as previously described [ 4 , 41 ]. Average lesion length and lesion width was used to represent a lesion size.…”

Section: Methodsmentioning

confidence: 99%

The Wall-Associated Receptor-Like Kinase TaWAK7D Is Required for Defense Responses to Rhizoctonia cerealis in Wheat

Zhu

Guo

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Sharp eyespot, caused by necrotrophic fungus Rhizoctonia cerealis, is a serious fungal disease in wheat (Triticum aestivum). Certain wall-associated receptor kinases (WAK) mediate resistance to diseases caused by biotrophic/hemibiotrophic pathogens in several plant species. Yet, none of wheat WAK genes with positive effect on the innate immune responses to R. cerealis has been reported. In this study, we identified a WAK gene TaWAK7D, located on chromosome 7D, and showed its positive regulatory role in the defense response to R. cerealis infection in wheat. RNA-seq and qRT-PCR analyses showed that TaWAK7D transcript abundance was elevated in wheat after R. cerealis inoculation and the induction in the stem was the highest among the tested organs. Additionally, TaWAK7D transcript levels were significantly elevated by pectin and chitin treatments. The knock-down of TaWAK7D transcript impaired resistance to R. cerealis and repressed the expression of five pathogenesis-related genes in wheat. The green fluorescent protein signal distribution assays indicated that TaWAK7D localized on the plasma membrane in wheat protoplasts. Thus, TaWAK7D, which is induced by R. cerealis, pectin and chitin stimuli, positively participates in defense responses to R. cerealis through modulating the expression of several pathogenesis-related genes in wheat.

show abstract

“…Additionally, the elongator subunit 4, which forms the elongator complex with five other subunits, including the acetyltransferase Elp3, is involved in histone acetylation in regions of defence genes associated with defence, including TaAGC1, TaCPK7‐D, TaPAL5, defensin, and chitinase 2. Therefore, it regulates the immune response to Rhizoctonia cerealis , the causal agent of sharp eyespot on wheat (Wang et al ., 2020).…”

Section: Protein Acetylation In Plant Immunitymentioning

confidence: 99%

Protein acetylation and deacetylation in plant‐pathogen interactions

Wang

Liu

Chen

et al. 2021

Environmental Microbiology

View full text Add to dashboard Cite

Protein acetylation and deacetylation catalyzed by lysine acetyltransferases (KATs) and deacetylases (KDACs) respectively, are major mechanisms regulating various cellular processes. During the fight between microbial pathogens and host plants, both apply a set of measures, including acetylation interference, to strengthen themselves while suppressing the other. In this review, we first summarize KATs and KDACs in plants and their pathogens. Next, we introduce diverse acetylation and deacetylation mechanisms affecting protein functions, including the regulation of enzyme activity and specificity, protein-protein or protein-DNA interactions, subcellular localization and protein stability. We then focus on the current understanding of acetylation and deacetylation in plant-pathogen interactions.Additionally, we also discuss potential acetylation-related approaches for controlling plant diseases.

show abstract

Wheat Elongator subunit 4 is required for epigenetic regulation of host immune response to Rhizoctonia cerealis

Cited by 9 publications

References 46 publications

The Wheat Wall-Associated Receptor-Like Kinase TaWAK-6D Mediates Broad Resistance to Two Fungal Pathogens Fusarium pseudograminearum and Rhizoctonia cerealis

The Wheat Wall-Associated Receptor-Like Kinase TaWAK-6D Mediates Broad Resistance to Two Fungal Pathogens Fusarium pseudograminearum and Rhizoctonia cerealis

The Wall-Associated Receptor-Like Kinase TaWAK7D Is Required for Defense Responses to Rhizoctonia cerealis in Wheat

Protein acetylation and deacetylation in plant‐pathogen interactions

Contact Info

Product

Resources

About