Wheat germ, a byproduct of the wheat milling industry, as a beneficial source of anti‐aging polyamines: A quantitative comparison of various forms

Abstract

Polyamines have received a lot of attention since the 1990s because of their anti‐aging, anti‐chronic disease, and proliferative effects. Wheat germ was reported as one of the natural sources of high polyamine, especially spermidine. The current study used three types of wheat germ: group A was industrially separated germ from whole grain, group B was the commercially available germinated wheat germ, and group C was manually separated wheat germ from germinated grain. The polyamine content of putrescine, spermidine, and spermine has been determined using a simplified isocratic LC–MS/MS method. An optimized extraction procedure was performed on all seven samples for obtaining a polyamine‐enriched extract. The three dominant carbomylated polyamines were identified by analyzing the extracted samples in order to determine their relative abundance. Wheat germ powders contain the highest amount of polyamines (220–337 μg/g) of which spermidine is one of the most important. Germinated wheat grains, on the other hand, contain the least amount of this polyamine. The commercially available separated wheat germs are suggested as a good nutrition source of these polyamines.

Comparison of the Polyamine Content of Five Spring Flowers with Wheat Germ as a Rich Anti-aging Polyamine Source for Preparation of Nutraceutical Products

“…Derivatization and analysis were carried out in accordance with our earlier work ( 33 ). Briefly, a 15 mL falcon tube containing 2 mL of toluene, 1 mL of phosphate buffer (0.5 M, pH 12), and 100 μL of IBCF was filled with 1 mL of the sample solution (100 mg/mL water) and 10 μL of 1, 6 diaminohexane solution (10 mg/mL water).…”

“…The following conditions were used throughout the analysis: The following parameters were used for mass spectrometry: spray voltage of 5.0 kV, capillary temperature of 320°C, gas flow rate of 8 L/min, and capillary voltage of 32 V. All analytes were found in positive mode during measurements of multiple reaction monitoring (MRM). Table 1 lists the analyte’s ideal analytical conditions ( 33 ).…”

“…Therefore, derivatization is favored in polyamine detection with MS/MS analyzers since it lowers interference and boosts repeatability ( 20 ). We provided a simple, shortened, and isocratic analysis that took advantage of derivatization techniques while avoiding the use of potent acids and typical interferences seen in classical LC-MS techniques ( 33 ).…”

Development and Characterization of High-Energy Protein Bars with Enhanced Antioxidant, Chemical, Nutritional, Physical, and Sensory Properties

“…Adding to that, previous studies have reported the anti-aging, anti-chronic disease, and proliferative effects of wheat germ [3]. Wheat germ is also known as an ergogenic aid due to its vitamin-E-rich content that can improve athlete performance [4].…”