Wheat Germ and Peanut Agglutinin Binding to Normal, Dysplastic and Neoplastic Cervical Epithelium

Bychkov, Vladimir; Toto, Patrick D.

doi:10.1159/000298947

Cited by 15 publications

(7 citation statements)

References 16 publications

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“…WGA and RCA-1 displayed marginal binding to glandular epithelium during the proliferative phase that increased substantially during the secretory phase. This same investigative group employed WGA and PNA as probes to analyze normal, dysplastic and neoplastic cervical epithelium [46]. They observed minimal binding of these lectins to normal squamous epithelium which increased substantially as the lesions became more malignant.…”

Section: Introductionmentioning

confidence: 99%

Functional glycosylation in the human and mammalian uterus

Clark

2015

Fertil Res and Pract

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BackgroundGlycosylation is the most common and structurally diverse of all the post-translational modifications of proteins. Lipids and extracellular matrices are also often glycosylated. The mammalian uterus is highly enriched in glycoconjugates that are associated with the apical surfaces of epithelial cells and the secretions released by both epithelial and stromal cells. These glycoconjugates interact primarily with sperm, the implanting embryo, the fetus, and any pathogen that happens to gain entry into the uterus. Secretions of the endometrial glands increase substantially during the luteal phase of the menstrual cycle. These secretions are highly enriched in glycoproteins and mucins that promote specific uterine functions.FindingsLectins and antibodies have been employed in the majority of the studies focused on uterine glycosylation have employed to define the expression of carbohydrate sequences. However, while these studies provide insight about potential glycosylation, precise information about glycan structure is lacking. Direct sequencing studies that employ biochemical or mass spectrometric methods are far more definitive, but have rarely been employed with uterine glycoproteins. Both lectin/antibody binding and direct carbohydrate sequencing studies that have been focused on the mammalian uterus are reviewed. The primary functional role of the eutherian uterus is to facilitate fertilization and nurture the developing embryo/fetus. Trophoblasts are the primary cells that mediate the binding of the embryo and placenta to the uterine lining. In mammals that utilize hemochorial placentation, they invade the decidua, the specialized endometrial lining that forms during pregnancy. Trophoblasts have also been analyzed for their lectin/antibody binding as a complement to the analysis of the uterine cells and tissues. They will also be reviewed here.ConclusionsThe functional roles of the glycans linked to uterine and trophoblast glycoconjugates remain enigmatic. Another major question in the human is whether defects in placental or uterine glycosylation play a role in the development the Great Obstetrical Syndromes. More recent findings indicate that changes in glycosylation occur in trophoblasts obtained from patients that develop preeclampsia and preterm birth. The functional significance of these changes remain to be defined. Whether such shifts happen during the development of other types of obstetrical syndromes remains to be determined.

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Section: Introductionmentioning

confidence: 99%

Functional glycosylation in the human and mammalian uterus

Clark

2015

Fertil Res and Pract

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“…Avidin-biotin-peroxidase complex tech nique was used [8,9]. Detailed descriptions of the method and commercial sources of the biotinylated lectins are reported in our previous communications [6,10]. Control of the staining reaction was obtained by treating sections with biotinyl-lectin solutions which were first reacted with their respective highaffinity glycosides and assessed for negative staining reactions.…”

Section: Methodsmentioning

confidence: 99%

Lectin Binding to Normal Human Endometrium

Bychkov¹,

Toto²

1986

Gynecol Obstet Invest

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Avidin-biotin-peroxidase labelling technique was used to localize the binding sites of peanut agglutinin (PNA), Ulexeuropaeus (UEA-I), wheat germ agglutinin (WGA), concanavalin A (Con A), and Ricinuscommunis (RCA-I) in 30 samples of endometrium at different stages of the menstrual cycle and in early pregnancy. PNA and UEA-I exhibit weak binding to the apical portions of epithelial cells in proliferative and secretory phases, but become strongly positive in pregnancy. WGA and RCA-I show weak binding to glandular epithelium in the proliferative phase, but increase their affinity in the secretory phase. Con A demonstrates strong affinity to decidual cells

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“…A prime example of aberrant glycosylation in carcinogenesis is the overexpression of sialyl Lewis A and sialyl Tn antigen in cancers of the pancreas, colon, stomach and esophagus [11], [12]. Moreover, increased sialytransferases and sialic acid content on cell glycoconjugates has long been linked to oral cancer and malignant transformation [13], [14].…”

Section: Introductionmentioning

confidence: 99%

“…One particular lectin of interest is the legume wheat germ agglutinin (WGA), which is a carbohydrate-binding lectin of approximately 36 kDa that selectively recognizes sialic acid and N-acetylglucosaminyl sugar residues [11], [14], [22]. Furthermore, conjugation of this lectin with a fluorophore could provide an effective non-invasive in vivo screening method to visualize premalignant and malignant oral lesions in real time.…”

Section: Introductionmentioning

confidence: 99%

Molecular Imaging of Oral Premalignant and Malignant Lesions Using Fluorescently Labeled Lectins

Baeten¹,

Suresh

Johnson³

et al. 2014

Translational Oncology

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Aberrant glycosylation during carcinogenesis results in altered glycan expression on oral cancer cells. The objective of this study was to detect this atypical glycosylation via imaging of fluorophore-conjugated lectins. Paired normal and tumor tissue from seven patients with oral squamous cell carcinoma were investigated for sialic acid expression via the legume protein wheat germ agglutinin (WGA). Fluorophore (Alexa Fluor 350 and Alexa Fluor 647) conjugated WGA was topically applied to the tissue samples and imaged using a custom wide-field fluorescence imaging system. All seven patients had histologically confirmed disease with 6/7 exhibiting squamous cell carcinoma and 1/7 exhibiting dysplasia. Fluorescent data collected from all patients demonstrated that fluorophore conjugated WGA could distinguish between pathologically normal and diseased tissue with the average signal-to-noise ratio (SNR) among all patients being 5.88 (P = .00046). This SNR was statistically significantly higher than the SNR from differences in tissue autofluorescence (P = .0049). A lectin inhibitory experiment confirmed that lectin binding is molecularly specific to overexpressed tumor glycans and that fluorescence is not due to tissue optical properties or tissue diffusion differences. These results illustrate that changes in tumor glycan content of oral neoplasms can be detected with optical imaging using topically applied fluorescently labeled WGA. Lectin targeting of oral lesions using optical imaging may provide a new avenue for the early detection of oral cancers.

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Wheat Germ and Peanut Agglutinin Binding to Normal, Dysplastic and Neoplastic Cervical Epithelium

Cited by 15 publications

References 16 publications

Functional glycosylation in the human and mammalian uterus

Functional glycosylation in the human and mammalian uterus

Lectin Binding to Normal Human Endometrium

Molecular Imaging of Oral Premalignant and Malignant Lesions Using Fluorescently Labeled Lectins

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