Wheat pathogenesis‐related proteins of class 4 have ribonuclease activity

Caporale, Carlo; Berardino, Iris Di; Leonardi, Luca; Bertini, Laura; Cascone, A; Buonocore, Vincenzo; Caruso, Carla

doi:10.1016/j.febslet.2004.07.091

Cited by 79 publications

(66 citation statements)

References 26 publications

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“…The content of soluble, insoluble and total protein in the shoots showed an increase in wheat plants inoculated with all the Fusarium species (Fig. 5), as also recorded by other authors (Carporale et al, 2004;Przymusinski et al, 2004), who indicated that plants respond to pathogens by defence strategies leading to the synthesis of protective compounds such as pathogenesis-related proteins. Even though 100 different proteins from the PR-10 family have been identified, their biological function has not yet been clarified and most of the roles suggested are highly hypothetical.…”

Section: Metabolic Activitysupporting

confidence: 84%

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

Gherbawy¹,

El-Tayeb²,

Maghraby

et al. 2012

Acta Agronomica Hungarica

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Inoculating wheat (cv. Giza 164) with various Fusarium species had different effects on the catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APX) activity in different plant tissues. The CAT activity induced in the shoots of wheat plants by some pathogens (F. merismoides, F. moniliforme, F. poae and F. sambucinum) was relatively higher than that induced by other pathogens. The results showed an increase in POX activity in infected shoots except those inoculated with F. sambucinum, where the POX activity decreased. Inoculating wheat plants with individual Fusarium species or with a mixture of pathogens caused an increase in APX in the shoots, except for those treated with F. poae, where the APX activity decreased. The activity of these antioxidant enzymes thus increased in most cases in the shoot system of wheat plants under Fusarium infection. Investigations on the metabolic activity of wheat plants inouculated with individual Fusarium species or with a mixture of pathogens showed higher contents of all the protein fractions, soluble sugars, total free amino acids and proline. The K + and Ca 2+concentrations decreased to various extents in different cases. The Na + content increased in wheat shoots, especially after inoculation with F. merismoides. The results signify that the most serious infection was caused by F. culmorum, F. graminearum and F. oxysporum and by a mixture of all the Fusarium species.

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Section: Metabolic Activitysupporting

confidence: 84%

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

Gherbawy¹,

El-Tayeb²,

Maghraby

et al. 2012

Acta Agronomica Hungarica

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“…A few PRs families have enzymatic effects such as chitinases (PR-3, PR-8, PR-11) 105,168 , glucanases (PR-2) 136,137 , peroxidases (PR-9) 2,132,331 , ribonucleases (PR-10) 85,190 and (PR-4) 41 , or inhibitory effects such as enzyme inhibitors (PR-6) 326,349 . Inhibitory activity towards proteases or α-amylases is ascribed to the PR-5 298 , PR-12 192 and PR-14 family 160,161 .…”

Section: Biological Function and Mechanism Of Actionmentioning

confidence: 99%

“…Binding of the PR-4 members with the chitin-binding domain to chitin in the developing fungal cell walls might result in fungal growth retardation. Ribonuclease activity of some cereal seed PR-4 has been observed and the effect on the invading microorganisms explained alternatively as inhibition of the translation process 41 .…”

Section: Barwin (Barley Wound Induced Protein) (Pr-4)mentioning

confidence: 99%

A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

Gorjanović

2009

Journal of the Institute of Brewing

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The mature barley seed proteome is dominated by proteins involved in stress responses, including the Pathogenesis-Related proteins (PRs). PRs are currently classified into 17 families. The biochemistry of the PRs families, whose members are constitutively present in barley seed, is surveyed in this paper. These proteins are assumed to protect dormant and germinating seeds against pathogenic microorganisms and pests. The current knowledge on PRs structural and functional properties is summarized in an attempt to illustrate their importance in barley seed innate resistance. At the same time, a platform to understand PRs technological function in cereal foods and in beverage processing and quality is provided.

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“…Data were processed as described previously 37 . Briefly, sequences were quality filtered and dereplicated using the QIIME script split_libraries.py with the homopolymer filter deactivated 38 , checked for chimeras against the GreenGenes database (October 2013 release) using UCHIME ver.…”

Section: Processing Of Sequence Datamentioning

confidence: 99%

“…36 PR4 proteins show antifungal activity against several phytopathogenic fungi and have been demonstrated to possess ribonucleasic activity correlated to their antifungal capacity. 37,38 PR5 encodes a thaumatin-like protein which exhibits antifungal activity against snow mold and Microdochium nivale. In shoots, PR5 (WAS-3a) was induced by 2.1-fold at 72 h post MeJA treatment ( Fig.2; Supplementary Fig.S1(f)).…”

Section: Pathogenesis-related Genesmentioning

confidence: 99%

Effects of strategic tillage and plant hormone treatments on wheat-associated microbial communities

Liu¹

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The main aim of this thesis was to characterise microbial properties of microbiomes associated with wheat (Triticum aestivum) soils and wheat plants. In particular my objective was to better understand the effects on microbial communities following strategic tillage (ST) in wheat field soils and the activation of plant defence pathways in wheat plants. Throughout the thesis, multiple culture-independent methods, especially next-generation sequencing of 16S rRNA gene were used to profile soil microbial communities. Over half of the arable land in the northern grains region of Australia is managed using no-till (NT), a farming method which has improved crop yields and soil quality while reducing the input and labour costs. However, in recent years, concerns have arisen among farmers over the weed control in continuous NT systems. Strategic tillage has been touted as a potential solution, in particular for the severe weed infestations of long-term NT. Nevertheless, there is little information on the influence of ST on the microbial properties of Australian NT soils.In the present study, ST applications and soil sampling were performed in Moonie, Moree and Condamine during the fallow period in eastern Australia. These sites were chosen based on their long history of repeated wheat cultivation under NT farming practices and their different soil types.Overall, results show that in the Moonie trial on a Calcisol, one-time ST with either chisel or offset disc did not significantly influence the composition of soil bacterial communities when measured 13 months after tillage. However, relative to the NT, chisel tillage led to significant increases in microbial biomass carbon (MBC), abundances of Alphaproteobacteria, Bacteroidetes and Firmicutes as well as the utilisation of D+cellubiose and mannitol at 0-10 cm depth. In the Moree trial on a grey Vertosol, ST with different timing and implement demonstrated great potential in weed control and did not influence wheat yield and soil physicochemical and biological properties in the short-term. In the Condamine site on a Solonetz soil, one-or two-time chisel tillage did not influence soil MBC, total microbial enzymatic activity (MEA) or utilisation of C substrates.Likewise, ST did not change the soil microbial community structure and the abundance of genes encoding enzymes involved in key steps of C and N reactions. However, one-time chisel increased relative abundance of Acidobacteria RB41 and Acidobacteria iii1-15, and two-time chisel slightly increased the average C utilisation, at 10-20 cm depth. Overall, this thesis suggests that ST does not cause major impacts on soil properties of long-term NT and could be potentially used to address the long-term NT-associated issues without impacting overall soil properties.Recent reports demonstrate the importance of microbiomes associated with plants and their soil they are cultivated in. Beneficial microbes can significantly increase crop yields and provide biocontrol functions against plant pathogens, but prior to this stu...

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Wheat pathogenesis‐related proteins of class 4 have ribonuclease activity

Cited by 79 publications

References 26 publications

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

Effects of strategic tillage and plant hormone treatments on wheat-associated microbial communities

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