Wheat sprout extract induces changes on 20S proteasomes functionality

Amici, Manila; Bonfili, Laura; Spina, Michele; Cecarini, Valentina; Calzuola, Isabella; Marsili, Valeria; Angeletti, Mauro; Fioretti, Evandro; Tacconi, R; Gianfranceschi, G.; Eleuteri, Anna Maria

doi:10.1016/j.biochi.2007.12.001

Cited by 34 publications

(22 citation statements)

References 54 publications

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“…In this study, six soluble phenolic compounds (gallic acid, p-coumaric acid, ferulic acid, 4-hydroxybenzoic acid, quercetin, and epicatachin) were detected in wheat sprouts exposed to different types of LEDs. Our results agree with those of previous studies reporting the presence of 4-hydroxybenzoic acid [32], ferulic acid [32], p-coumaric acid [32], gallic acid [33], and epicatechin [33] in wheat sprouts. Under all the light conditions used in this study, the contents of gallic acid, 4-hydroxybenzoic acid, p-coumaric acid, and quercetin were gradually increased and reached a maximum at 12 days; this coincided with the expression of TaPAL2, TaC4H, Ta4CL1, TaCHS, and TaF3H, which was increased at 4 to 12 days.…”

Section: Discussionsupporting

confidence: 93%

Effects of LED lights on Expression of Genes Involved in Phenylpropanoid Biosynthesis and Accumulation of Phenylpropanoids in Wheat Sprout

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Park

et al. 2019

Agronomy

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Phenylpropanoids are naturally occurring compounds that exert beneficial pharmacological effects on human health. Phenylpropanoids can act as antioxidants and are involved in resistance to ultraviolet light and cancer; these compounds possess anti-inflammatory, antiviral, and antibacterial activity, and aid in wound healing. The expression of genes involved in phenylpropanoid biosynthesis, and consequent accumulation of phenylpropanoids in wheat sprout under conditions of stress, have not been extensively studied. This is the first study to examine the effects of light-emitting diodes (LED) on the expression of genes involved in phenylpropanoid biosynthesis and accumulation of phenylpropanoids in wheat sprouts. Our results, obtained using qRT-PCR and HPLC analyses, indicate that white light (380 nm) was the optimal wavelength for epicatechin biosynthesis in wheat sprouts. Compared with the effects of white light, blue light (470 nm) enhanced the accumulation of gallic acid and quercetin, but decreased the levels of p-coumaric acid and epicatechin; red light (660 nm) increased the accumulation of ferulic acid at 8 day and p-coumaric acid at 12 day. Compared gene expression with phenylpropanoid content showed that TaPAL3, TaPAL4, and TaDFR maybe important genes in phenylpropanoid biosynthesis in wheat sprout. This study provides insights into the effects of led lights on phenylpropanoid production in wheat sprouts. This knowledge will help improve secondary metabolite production in wheat sprouts.

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Section: Discussionsupporting

confidence: 93%

Effects of LED lights on Expression of Genes Involved in Phenylpropanoid Biosynthesis and Accumulation of Phenylpropanoids in Wheat Sprout

Cường

Park

et al. 2019

Agronomy

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“…We recently found that wheat sprout hydroalcoholic extract, rich in catechin, epicatechin and epigallocatechin gallate, can induce gradual inhibition of the 20 S proteasome ChT‐L, T‐L, PGPH and BrAAP components. Wheat sprout extract affected proteasome functionality in a Caco cell line and it influenced the expression of pro‐apoptotic proteins [157]. We also demonstrated that tumour cell line proteasomes showed a higher degree of impairment with respect to normal cell proteasomes, upon wheat sprout extract polyphenol and peptide components treatment (unpublished data).…”

Section: Proteasome Modulation By Flavonoidsmentioning

confidence: 56%

Natural polyphenols as proteasome modulators and their role as anti‐cancer compounds

et al. 2008

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The purpose of this review is to discuss the effect of natural antioxidant compounds as modulators of the 20S proteasome, a multi‐enzymatic multi‐catalytic complex present in the cytoplasm and nucleus of eukaryotic cells and involved in several cellular activities such as cell‐cycle progression, proliferation and the degradation of oxidized and damaged proteins. From this perspective, proteasome inhibition is a promising approach to anticancer therapy and such natural antioxidant effectors can be considered as potential relevant adjuvants and pharmacological models in the study of new drugs.

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“…Data reported in Table 2 show the polyphenol concentrations present in the selected germinated grains and non-germinated methanolic extract with high catechin content and ferulic acid and luteolin were detected in all grain extracts. Amici et al (2008) similarly reported high catechins content, particularly catechin, epicatechin, epigallocatechin and epigallocatechingallate in the extracts. Others have reported four polyphenol catechins in green tea (C, EGC, EC and EGCG).…”

Section: Analysis Of Polyphenolsmentioning

confidence: 74%

“…Quantification of polyphenols in grain extracts were performed using HPLC as described by Amici et al (2008). A Varian HPLC system (Varian Analytical Instruments, Walnut Creek, CA, USA) equipped with a C 18 Phenomenex Luna column (2.5 mm porosity, 250 Â 4 mm, with an UltraSep ES RP18 pre-column) was used with a three-step linear gradient for the separation of all polyphenols in the samples.…”

Section: Determination Of Individual Polyphenolsmentioning

confidence: 99%