When sperm meets egg

Melcher, Karsten

doi:10.1038/nature18448

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…Izumo1 carries a β-hairpin region that anchors two folded α-helix domains. Izumo1 is structurally stabilized after binding to Juno, as it brings about conformational change in the flexible Izumo1 β-hairpin region, which becomes elongated ( Aydin et al ., 2016 ; Melcher, 2016 ; Ohto et al ., 2016 ). It has been suggested that binding to Juno promotes Izumo1 dimerization, subsequently preparing for oocyte-sperm membrane fusion ( Inoue et al ., 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Location and expression of Juno in mice oocytes during maturation

Suzuki

Sugano

Ito

et al. 2017

JBRA Assisted Reproduction

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ObjectiveOocyte-sperm interaction is the essential step in fertilization. Juno, which has been known as Folate receptor 4, is the Izumo1 receptor expressed on the oocyte membrane. This study aims to investigate the location and expression of Juno in mice oocytes during maturation.MethodsTo confirm the stage at which Juno expression begins in the mice oocytes and its location pattern, we performed immunostaining methods. Next, we evaluated Juno mRNA expression by a half quantitative RT-PCR. Juno knockdown oocytes were generated by microinjecting siRNA into the germinal vesicle (GV) stage oocytes, and analyzed the maturation rate.ResultsOur results showed that Juno was expressed on the surface of the oocyte cytoplasmic membrane at the GV stage and it continues to be expressed at similar levels in the metaphase II (MII) stages of oocytes maturation. Interestingly, Juno is also expressed on the first polar body membrane at the MII stage. Fluorescence showing Juno expression was decreased in the oolemma of siRNA injected oocytes, but it was not completely disappearing in knock down oocytes. MII stage-rates of siRNA injected oocytes were not significantly different from sham controls.ConclusionJuno was expressed in oocytes at the GV stage and it continues to be expressed at similar levels in later stages of oocytes maturation. Juno accumulation in oolemma during oocyte maturation is essential for fertilization, such as membrane recognition of both gametes.

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Section: Introductionmentioning

confidence: 99%

Location and expression of Juno in mice oocytes during maturation

Suzuki

Sugano

Ito

et al. 2017

JBRA Assisted Reproduction

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Chapter 14 Technical and Ethical Limits in Prenatal and Preimplantation Genetic Diagnosis

Karbarz

2018

The Ethics of Reproductive Genetics

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Mini Review; Differentiation of Human Pluripotent Stem Cells into Oocytes

Wang

Farzaneh

2020

CSCR

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Primary Ovarian Insufficiency (POI) is one of the main diseases causing female infertility that occurs in about 1% of women between 30-40 years of age. There are few effective methods for the treatment of women with POI. In the past few years, stem cell-based therapy as one of the most highly investigated new therapies has emerged as a promising strategy for the treatment of POI. Human pluripotent stem cells (hPSCs) can self-renew indefinitely and differentiate into any type of cell. Human Embryonic Stem Cells (hESCs) as a type of pluripotent stem cells are the most powerful candidate for the treatment of POI. Human-induced Pluripotent Stem Cells (hiPSCs) are derived from adult somatic cells by the treatment with exogenous defined factors to create an embryonic-like pluripotent state. Both hiPSCs and hESCs can proliferate and give rise to ectodermal, mesodermal, endodermal, and germ cell lineages. After ovarian stimulation, the number of available oocytes is limited and the yield of total oocytes with high quality is low. Therefore, a robust and reproducible in-vitro culture system that supports the differentiation of human oocytes from PSCs is necessary. Very few studies have focused on the derivation of oocyte-like cells from hiPSCs and the details of hPSCs differentiation into oocytes have not been fully investigated. Therefore, in this review, we focus on the differentiation potential of hPSCs into human oocyte-like cells.

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When sperm meets egg

Cited by 3 publications

References 12 publications

Location and expression of Juno in mice oocytes during maturation

Location and expression of Juno in mice oocytes during maturation

Chapter 14 Technical and Ethical Limits in Prenatal and Preimplantation Genetic Diagnosis

Mini Review; Differentiation of Human Pluripotent Stem Cells into Oocytes

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