Whey protein suppresses the osteoclast-mediated bone resorption and osteoclast cell formation

Takada, Yukihiro; Kobayashi, Naomichi; Matsuyama, Hiroaki; Kato, Ken; Yamamura, Jun-ichi; Yahiro, Masatoshi; Kumegawa, Masayoshi; Aoe, Seiichiro

doi:10.1016/s0958-6946(97)00073-3

Cited by 57 publications

(39 citation statements)

References 6 publications

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“…7) Theseˆndings were consistent with our current knowledge of responses of osteoclasts in vitro. 2,5) We also found that cystatin, puriˆed from MBP, suppressed osteoclast-mediated bone resorption (unpublished data). It was earlier reported that recombinant cystatin C inhibited bone resorption in vitro.…”

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confidence: 70%

“…Recent in vitro and in vivo studies have shown that milk whey protein, especially its basic protein fraction, contains several components capable of both promoting bone formation and inhibiting bone resorption and further have demonstrated that milk whey protein plays a functional role in bone remodeling. 1,2) In these reports, the active components responsible for promotion of bone formation and suppression of bone resorption were characterized as its basic protein fraction (milk basic protein, MBP). In vivo studies in our laboratory showed that the milk whey protein and fractionated whey protein increased femoral bone strength in young ovariectomized rats.…”

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confidence: 99%

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Milk Basic Protein (MBP) Increases Radial Bone Mineral Density in Healthy Adult Women

Yamamura¹,

Aoe²,

Toba³

et al. 2002

Bioscience, Biotechnology, and Biochemistry

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confidence: 70%

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confidence: 99%

Milk Basic Protein (MBP) Increases Radial Bone Mineral Density in Healthy Adult Women

Yamamura¹,

Aoe²,

Toba³

et al. 2002

Bioscience, Biotechnology, and Biochemistry

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“…Milk, but not meat, decreases bone turnover AZ Budek et al 1996), suppress osteoclast-mediated bone resorption and decrease formation of osteoclasts (Takada et al, 1997). Similarly, in a randomized placebo-controlled human study, whey acidic fractions significantly decreased (Po0.05) u-NTX and u-DPD, however, did not significantly affect s-OC and s-BAP, in young women (Aoe et al, 2001).…”

Section: Discussionmentioning

confidence: 96%

High intake of milk, but not meat, decreases bone turnover in prepubertal boys after 7 days

et al. 2007

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Objective: To compare the short-term effect of a high milk and a high meat intake, identical in protein amount, on bone turnover during prepuberty. Setting: A University Department. Design and Subjects: From 28, randomly recruited, 8-year-old boys, first 14 were assigned to the milk group and next 14 to the meat group. In each group, 12 boys finished the dietary intervention. Intervention: Milk (1.5 l/day) and meat (250 g/d), both containing B53 g of protein, were given together with the habitual diet for 7 days. At baseline and dayÀ7, serum osteocalcin (s-OC), bone-specific alkaline phosphatase (s-BAP) and C-terminal telopeptides of type I collagen (s-CTX) were measured (immunoassay) and dietary intake was estimated (a 3-day weighted food record). Results: Baseline s-OC, s-BAP and s-CTX were not significantly different between the groups. After 7 days, the average protein intake increased in both groups by 47.5 g; the milk group had higher (Po0.0001) calcium intake; s-OC and s-CTX decreased (Pp0.04) in the milk group (À30.9%; À18.7%, respectively) compared with the meat group ( þ 6.4%; À1.0%, respectively) and s-BAP decreased (P ¼ 0.06) both in the milk (À3.9%) and the meat group (À7.5%). Conclusions: At the equal protein intake, milk, but not meat, decreased bone turnover in prepubertal boys after 7 days. This effect was probably due to some milk-derived compounds, rather than to the total protein intake. Future studies should elucidate the mechanism(s) of milk-related decline of bone turnover and its relevance for peak bone mass during growth. Sponsorship: University PhD scholarships.

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“…Chromatography methods used for the separation of growth factors and IgG from milk, whey or colostrum. Milk basic protein (MBP) Whey Cationic-exchange (mono S) [216] TGF-β2 B o v i n e platelets Ethanol-acid extraction from platelets and CM sepharose [201] TGF-β, IGF-I Milk products Cation exchange resins + hydroxyapatite column [106] TGF-β1, TGF-β2 Bone tissue Cation-exchange resins + RP-HPLC, heparin column, thiophillic resin [98] TGF-β ? Heparin-like resin + butyl sepharose-like resin [57] 112 S.F.…”

Section: Approaches To Extracting Milk Growth Factorsmentioning

confidence: 99%

Growth factors from bovine milk and colostrum: composition, extraction and biological activities

Gauthier

Pouliot

Maubois

2006

Lait

125

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-EGF, BTC, IGF-I, IGF-II, TGF-β1, TGF-β2, FGF1 and 2, and PDGF are the main growth factors present in bovine milk and colostrum. All of these growth factors are also found in human milk but at a lower concentration. The various compositional data reported in the literature vary greatly but it is evidenced that the day of lactation has the most important effect. Milk growth factors are characterized by a neutral to alkaline isoelectric point (pI) and a molecular mass between 6400 g·mol -1 and 30000 g·mol -1 . However, many of the growth factors are in a latent form, bound to high-molecular-mass proteins. Milk growth factors are resistant generally to pasteurization but disulfide reducing agents have been found to inactivate some species such as TGF-β's. The published data on bioavailability of milk growth factors are somewhat contradictory but it is generally accepted that they are resistant to gastric digestion and they can exert local and systemic effects on the gastrointestinal tract. Cation-exchange chromatography has been widely used for the extraction of milk growth factors because of the basic nature of these molecules. Membrane separations such as microfiltration (MF) have also been used successfully for the extraction of immunoglobulins and of some growth factors from colostrum, while ultrafiltration (UF) was successful only at separating IGF-I and IGF-II in whey. Milk growth factor extracts have been developed for various applications such as treatment of gastrointestinal disorders and skin diseases, wound healing, and induction of oral tolerance.

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Whey protein suppresses the osteoclast-mediated bone resorption and osteoclast cell formation

Cited by 57 publications

References 6 publications

Milk Basic Protein (MBP) Increases Radial Bone Mineral Density in Healthy Adult Women

Milk Basic Protein (MBP) Increases Radial Bone Mineral Density in Healthy Adult Women

High intake of milk, but not meat, decreases bone turnover in prepubertal boys after 7 days

Growth factors from bovine milk and colostrum: composition, extraction and biological activities

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